Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Leny Heliawati

Judul : CYTOTOXIC ACTIVITY AGAINST P-388 MURINE CELLS FROM THE SEEDS OF GEWANG (CORYPHA UTAN LAMK)

Abstrak :
Gewang (Corypha utan Lamk.) is a plant that grows wild in savanna area of â€‹â€‹East Nusa Tenggara (NTT). Gewang fruit is traditionally used by the people of Timor, NTT, as fish poison. This empirical fact gives an early indication of the presence of active toxic compounds in the Gewang plant. The purpose of this study was to isolate the active compounds in seed and knowing Gewang cytotoxic activity against cancer cells P-388 murine leukemia. Isolation methods started with maceration of Gewang seed with methanol. Methanol extract was then separated and purified by chromatography methods. The resulted isolates was evaluated by cytotoxicity test against Murin leukemia cancer cells P-388 and characterized using UV spectrometer, FTIR, and NMR (1H NMR, 13C NMR, 1D NMR and 2D NMR). Isolation obtained was yellowish solids as much as 15 mg and based on the results of the characterization indicated that isolates was suspected as Resveratrol compound with the formula of C14H13O3. Result of cytotoxicity test against Murin leukemia cancer cells P-388 showed IC50 value = 4,0 ppm which is meant that the compound can be categorized as active compound, hence it has good prospects as anticancer.

Tahun : 2013 Media Publikasi : Prosiding

Kategori : Prosiding No/Vol/Tahun : - / - / 2013

ISSN/ISBN : -

PTN/S : Universitas Pakuan Program Studi : KIMIA

Bibliography :
Alley, M. C., Scudiero,D. A., Monks,A., Hursey, M. L., Czerwinski, M.J., Fine, D. L., Abbott, B. J., Mayo, J. G., Shoemaker, R. H., and Boyd., M. R. 1988. Feasibility of drug screening with panels of tumor cell lines using a microculturetetrazolium assay. Cancer Research 48: 589-601.

Hostettman, K . 1991, Assay of bioactivity. Methode in plant biochemsitry, series editor P. M. Dey, J. B. hardorne, Vol 6, Academic Press, London.

Lee, Yu Young; Kwan, Soon Ho; Park, Hyo Jun; Yang, Eun Ju; Kim, Sung Kook; Yoon, Young Hwan; Kim, Cung Guk; Park, Jong Wook and Kyung Sik Song. 2009. Isolation of Uleanane Triterpenes and trans-Resveratrol from the root of peanut (Arachis hypogaea). J. Korean. Soc. Appl. Bio. Chem. 52 (1). 40-44.

Nihal, A. and Mukhtar H. 1999. Green tea polyphenols and cancer, biologic mechanism and practical implications. Nutrition Review 57 (3), 76-78.

URL : https://repository.unpak.ac.id/

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Detail Karya Ilmiah Dosen

Leny Heliawati

Judul	:	CYTOTOXIC ACTIVITY AGAINST P-388 MURINE CELLS FROM THE SEEDS OF GEWANG (CORYPHA UTAN LAMK)
Abstrak	:	Gewang (Corypha utan Lamk.) is a plant that grows wild in savanna area of â€‹â€‹East Nusa Tenggara (NTT). Gewang fruit is traditionally used by the people of Timor, NTT, as fish poison. This empirical fact gives an early indication of the presence of active toxic compounds in the Gewang plant. The purpose of this study was to isolate the active compounds in seed and knowing Gewang cytotoxic activity against cancer cells P-388 murine leukemia. Isolation methods started with maceration of Gewang seed with methanol. Methanol extract was then separated and purified by chromatography methods. The resulted isolates was evaluated by cytotoxicity test against Murin leukemia cancer cells P-388 and characterized using UV spectrometer, FTIR, and NMR (1H NMR, 13C NMR, 1D NMR and 2D NMR). Isolation obtained was yellowish solids as much as 15 mg and based on the results of the characterization indicated that isolates was suspected as Resveratrol compound with the formula of C14H13O3. Result of cytotoxicity test against Murin leukemia cancer cells P-388 showed IC50 value = 4,0 ppm which is meant that the compound can be categorized as active compound, hence it has good prospects as anticancer.
Tahun	:	2013	Media Publikasi	:	Prosiding
Kategori	:	Prosiding	No/Vol/Tahun	:	- / - / 2013
ISSN/ISBN	:	-
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	Alley, M. C., Scudiero,D. A., Monks,A., Hursey, M. L., Czerwinski, M.J., Fine, D. L., Abbott, B. J., Mayo, J. G., Shoemaker, R. H., and Boyd., M. R. 1988. Feasibility of drug screening with panels of tumor cell lines using a microculturetetrazolium assay. Cancer Research 48: 589-601. Hostettman, K . 1991, Assay of bioactivity. Methode in plant biochemsitry, series editor P. M. Dey, J. B. hardorne, Vol 6, Academic Press, London. Lee, Yu Young; Kwan, Soon Ho; Park, Hyo Jun; Yang, Eun Ju; Kim, Sung Kook; Yoon, Young Hwan; Kim, Cung Guk; Park, Jong Wook and Kyung Sik Song. 2009. Isolation of Uleanane Triterpenes and trans-Resveratrol from the root of peanut (Arachis hypogaea). J. Korean. Soc. Appl. Bio. Chem. 52 (1). 40-44. Nihal, A. and Mukhtar H. 1999. Green tea polyphenols and cancer, biologic mechanism and practical implications. Nutrition Review 57 (3), 76-78.
URL	:	https://repository.unpak.ac.id/