Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Tri Saptari Haryani, Triastinurmiatiningsih, Moerfiah, Fitra Akbar Nugraha

Judul : THE TEST OF Padina australis-BASED TABLET AS AN ANTI-DIARRHEAL MEDICINE ON Escherichia coli INDUCED MALE SPRAGUE DAWLEY RATS

Abstrak :
Diarrhea is a process of discharging feces from the body (defecation) in which the feces is half liquid or liquid-shaped and more watery than normal. One of its cause is the Escherichia coli bacteria. Diarrhea is often treated using chemical medicine like Loperamid, but because of its many side effects, people start using herbal medicine instead. Padina australis is a species of seaweed that can be used as a medicine, specifically to reduce the growth of Escherichia coli, which is a diarrhea-caused bacterium. This research aims to determine the effect of Padina australis-based tablet and its optimal dosage as an anti-diarrheal medicine. This research use tablets made from Padina australis extract and 30 male Sprague Dawley rat with age 2-3 months and weight ± 250 gr as the test subject. The method used in this research includes environmental adaptation on the test subject, producing Padina australis extract tablet suspension, testing tablet suspension on various concentrations, macroscopic and microscopic examination of test subject’s intestines using intestinal transit method, and histopathology examination of test subject’s organ. The result indicates that 3.78 mg/200 gr weights is the most optimal dosage to restore the condition of test subject’s intestine. Microscopic examination shows no tissue damage on the test subject’s digestive organs. Therefore, we conclude that Padina australis extract tablet has an anti-diarrheal effect on male Sprague Dawley rat with 3.78 mg/200 gr weights as the most optimal dosage.

Keyword: anti-diarrhea, male rat digestive organs, Padina australis extract tablet.

Tahun : 2018 Media Publikasi : Seminar Internasional

Kategori : Jurnal No/Vol/Tahun : - / - / -

ISSN/ISBN : -

PTN/S : Universitas Pakuan Program Studi : BIOLOGI

Bibliography :
Anas, Y., Fitria, F.R., Purnamasari, A.Y., Ningsih, A.K, Noviantoro, G.A., and Suharjono. 2012. Aktivitas Antidiare Ekstrak Etanol Daun Randu (Ceiba petandra L. Gaern.) pada Mencit Jantan Galur Balb/C. Semarang: Fakultas Farmasi Universitas Wahid Hasyim dan Fakultas Kedokteran Universitas Diponegoro. Halaman 16-22.

Corwin, E.J. 2009. Buku Saku Patofisiologi Corwin. Edisi ke 3. EGC. Jakarta. hal.235

Dewoto, H.R. 2007. Farmakologi dan Terapi. Editor: Sulistina Ganiswarma. Jakarta: Penerbit FKUI. Hal. 221.

Di Carlo, G., Autore, G., Izzo, A.A., Maiolino, P., Mascolo, N., Viola, P., Diurno, M.V., dan Capasso, F. 1993. Inhibition of Intestinal Motility and Secretory by Flavonoids in Mice and Rats: Structure Activity Relationships. Journal of Pharmacy and Pharmacology. 45(12): 1054- 1059.

Hanura, DL., Lohitasari, B., Tri Saptari. H. 2016. Efektivitas Formula Tablet Ekstrak Padina australis Sebagai Antibakteri Escherichia coli. Universitas Pakuan, Bogor.

Inayathulla, Shariff W.R., Asif, K., and Mukesh, S. 2010. Evaluation of Antidiarrhoeal Activity of Crataeva nurvala Root Bark in Experimental Animals. India: International Journal of Pharmacy and Pharmaceutical Sciences. Vol. 2. (1): 158-161.

Janda, J.M, & Abbott SL. 2006. The Enterobacteria. Second Ed. Washington: ASM Press.

Junqueira, L.C. and Carneiro, J. 2007. Histologi Dasar Teks & Atlas. Edisi 10. Alih Bahasa: Jan Tambayong. Editor: Frans Dany. Jakarta: Penerbit Buku Kedokteran EGC.

Lu, F.C. 2001. Toksikologi dasar Asas, Organ Sasaran dan Penilaian Risiko. Edisi 2. Jakarta: UI-Press.

Miranda, R.N, & Khoudry, J.D. 2013. Atlas of Limph Node Pathology.

Purwaningdyah, Y.G., Tri D.W., Novita W., 2015, Efektivitas Ekstrak Biji Pepaya (Carica papaya L.) sebagai Antidiare pada Tikus yang di Induksi Salmonella thyphimurium, Jurnal Pangan dan Agroindustri,Vol. 3 (4): Hal. 1283-1293.

Rajha H.N., Darra N. El, Hobaika Z., Boussetta N., Vorobiev E., Maroun R.G. and Louka N., 2014, Extraction of Total Phenolic Compounds, Flavonoids, Anthocyanins and Tannins from Grape Byproducts by Response Surface Methodology. Influence of Solid-Liquid Ratio, Particle Size, Time, Temperature and Solvent Mixtures on the Optimization Process, Food and Nutrition Sciences, 2014, 397–409.

Savkovic, S.D, Villanueva J, Turner JR, Matkowskyj KA, Hecht G. 2005. Mouse model of enteropathogenic Escherichia coli infection. Infection and Immunity 73: 1161-1170.

Suwito, Widodo. 2010. Deteksi Escherichia coli O157: H7 Dari Susu dan Daging Menggunakan serum Kebal Monospesifik. Jurnal Sain Vet, 28(2).

Tjay, H.T., dan Rahardja, K. 2007. Obat-obat Penting. Khasiat, Penggunaan, dan Efek-efek Sampingnya. Edisi Keenam. Cetakan Pertama. Jakarta: Elex Media Komputindo. Halaman 288-289.

Triastinurmiatiningsih dan Tri Saptari Haryani. 2008. Potensi Rumput Laut di Pantai Bayah, Kabupaten Lebak, Banten Sebagai Anti Bakteri Escherichia coli. Jurnal Matematika, Sains dan Teknologi, Vol 9 (1): hal 37-43.

Zein, U., Khalid, H. dan Josia, G. 2004. Diare Akut Disebabkan Bakteri. Dalam: e-USU Repository Universitas Sumatra Utara.

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Detail Karya Ilmiah Dosen

Tri Saptari Haryani, Triastinurmiatiningsih, Moerfiah, Fitra Akbar Nugraha

Judul	:	THE TEST OF Padina australis-BASED TABLET AS AN ANTI-DIARRHEAL MEDICINE ON Escherichia coli INDUCED MALE SPRAGUE DAWLEY RATS
Abstrak	:	Diarrhea is a process of discharging feces from the body (defecation) in which the feces is half liquid or liquid-shaped and more watery than normal. One of its cause is the Escherichia coli bacteria. Diarrhea is often treated using chemical medicine like Loperamid, but because of its many side effects, people start using herbal medicine instead. Padina australis is a species of seaweed that can be used as a medicine, specifically to reduce the growth of Escherichia coli, which is a diarrhea-caused bacterium. This research aims to determine the effect of Padina australis-based tablet and its optimal dosage as an anti-diarrheal medicine. This research use tablets made from Padina australis extract and 30 male Sprague Dawley rat with age 2-3 months and weight ± 250 gr as the test subject. The method used in this research includes environmental adaptation on the test subject, producing Padina australis extract tablet suspension, testing tablet suspension on various concentrations, macroscopic and microscopic examination of test subject’s intestines using intestinal transit method, and histopathology examination of test subject’s organ. The result indicates that 3.78 mg/200 gr weights is the most optimal dosage to restore the condition of test subject’s intestine. Microscopic examination shows no tissue damage on the test subject’s digestive organs. Therefore, we conclude that Padina australis extract tablet has an anti-diarrheal effect on male Sprague Dawley rat with 3.78 mg/200 gr weights as the most optimal dosage. Keyword: anti-diarrhea, male rat digestive organs, Padina australis extract tablet.
Tahun	:	2018	Media Publikasi	:	Seminar Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	- / - / -
ISSN/ISBN	:	-
PTN/S	:	Universitas Pakuan	Program Studi	:	BIOLOGI
Bibliography	:	Anas, Y., Fitria, F.R., Purnamasari, A.Y., Ningsih, A.K, Noviantoro, G.A., and Suharjono. 2012. Aktivitas Antidiare Ekstrak Etanol Daun Randu (Ceiba petandra L. Gaern.) pada Mencit Jantan Galur Balb/C. Semarang: Fakultas Farmasi Universitas Wahid Hasyim dan Fakultas Kedokteran Universitas Diponegoro. Halaman 16-22. Corwin, E.J. 2009. Buku Saku Patofisiologi Corwin. Edisi ke 3. EGC. Jakarta. hal.235 Dewoto, H.R. 2007. Farmakologi dan Terapi. Editor: Sulistina Ganiswarma. Jakarta: Penerbit FKUI. Hal. 221. Di Carlo, G., Autore, G., Izzo, A.A., Maiolino, P., Mascolo, N., Viola, P., Diurno, M.V., dan Capasso, F. 1993. Inhibition of Intestinal Motility and Secretory by Flavonoids in Mice and Rats: Structure Activity Relationships. Journal of Pharmacy and Pharmacology. 45(12): 1054- 1059. Hanura, DL., Lohitasari, B., Tri Saptari. H. 2016. Efektivitas Formula Tablet Ekstrak Padina australis Sebagai Antibakteri Escherichia coli. Universitas Pakuan, Bogor. Inayathulla, Shariff W.R., Asif, K., and Mukesh, S. 2010. Evaluation of Antidiarrhoeal Activity of Crataeva nurvala Root Bark in Experimental Animals. India: International Journal of Pharmacy and Pharmaceutical Sciences. Vol. 2. (1): 158-161. Janda, J.M, & Abbott SL. 2006. The Enterobacteria. Second Ed. Washington: ASM Press. Junqueira, L.C. and Carneiro, J. 2007. Histologi Dasar Teks & Atlas. Edisi 10. Alih Bahasa: Jan Tambayong. Editor: Frans Dany. Jakarta: Penerbit Buku Kedokteran EGC. Lu, F.C. 2001. Toksikologi dasar Asas, Organ Sasaran dan Penilaian Risiko. Edisi 2. Jakarta: UI-Press. Miranda, R.N, & Khoudry, J.D. 2013. Atlas of Limph Node Pathology. Purwaningdyah, Y.G., Tri D.W., Novita W., 2015, Efektivitas Ekstrak Biji Pepaya (Carica papaya L.) sebagai Antidiare pada Tikus yang di Induksi Salmonella thyphimurium, Jurnal Pangan dan Agroindustri,Vol. 3 (4): Hal. 1283-1293. Rajha H.N., Darra N. El, Hobaika Z., Boussetta N., Vorobiev E., Maroun R.G. and Louka N., 2014, Extraction of Total Phenolic Compounds, Flavonoids, Anthocyanins and Tannins from Grape Byproducts by Response Surface Methodology. Influence of Solid-Liquid Ratio, Particle Size, Time, Temperature and Solvent Mixtures on the Optimization Process, Food and Nutrition Sciences, 2014, 397–409. Savkovic, S.D, Villanueva J, Turner JR, Matkowskyj KA, Hecht G. 2005. Mouse model of enteropathogenic Escherichia coli infection. Infection and Immunity 73: 1161-1170. Suwito, Widodo. 2010. Deteksi Escherichia coli O157: H7 Dari Susu dan Daging Menggunakan serum Kebal Monospesifik. Jurnal Sain Vet, 28(2). Tjay, H.T., dan Rahardja, K. 2007. Obat-obat Penting. Khasiat, Penggunaan, dan Efek-efek Sampingnya. Edisi Keenam. Cetakan Pertama. Jakarta: Elex Media Komputindo. Halaman 288-289. Triastinurmiatiningsih dan Tri Saptari Haryani. 2008. Potensi Rumput Laut di Pantai Bayah, Kabupaten Lebak, Banten Sebagai Anti Bakteri Escherichia coli. Jurnal Matematika, Sains dan Teknologi, Vol 9 (1): hal 37-43. Zein, U., Khalid, H. dan Josia, G. 2004. Diare Akut Disebabkan Bakteri. Dalam: e-USU Repository Universitas Sumatra Utara.
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