Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Tri Aminingsih, Husain Nashrianto, Aji Syaiful Rohman

Judul : POTENSI ANTIBAKTERI TERHADAP Escherichia coli DAN Staphylococcus aureus DAN IDENTIFIKASI SENYAWA EKSTRAK HEKSANA BANDOTAN (Ageratum conyzoides L.) Tri

Abstrak :
Bandotan (Ageratum conyzoides L.) is a weed plant that is often destroyed, but there is a group of people who use this plant as a traditional medicine that can cure some kinds of diseases such as scab lesions on the skin, malaria, influenza, pneumonia, tumors, and rheumatoid drug. This study aims to identify the substances present in the hexane extract bandotan and test antibacterial activity against Staphylococcus aureus and Escherichia coli. Bandotan herb extracted with hexane using maceration method. Maceration is done in a closed vessel for 24 hours and occasional stirring. Maceration process is performed three times the volume of 500 mL. Hexane extract was concentrated with a rotary evaporator and phytochemical testing includes group alkaloids, saponins, tannins, triterpenoids steroids and flavonoids. Bandotan hexane extract tested antibacterial activity against Staphylococcus aureus and Escherichia coli with a paper disc method and analyzed its chemical compounds by Gas Chromatography-mass spectrophotometry (GC-MS). The results showed that the content of the extract yield 10.01%, 8.41% moisture content, and the hexane extract contains compounds bandotan alkaloids, triterpenoids-steroids and flavonoids. Antibacterial activity with a diameter of inhibition (DDH) against S. aureus and E. coli 29.6 mm 12.4 mm making it more susceptible to S. aureus (gram positive bacteria) compared with E. coli (gram negative bacteria). Component compounds contained in the bandotan hexane extract among others kariofilen, isokariofilen, ageratokromen, demetoksiageratokromen, 6-vinyl-7methoxy-2,2-dimetilkromen, coumarin, dichloroacetic acid, 1-heptadekanol, and 7-ethyl-6methyl 5-metiltiopirazolo [1,5-a] pyrimidine. These compounds are thought to compound that acts as an antibacterial agent.

Keywords : Ageratum Conyzoides L., hexane, antibacterial, Escherichia coli, GC-MS Staphylococcus aureus

Tahun : 2012 Media Publikasi : jurnal nasional terakreditasi B

Kategori : Jurnal No/Vol/Tahun : 1 / 2 / 2012

ISSN/ISBN : 2087-9164

PTN/S : Universitas Pakuan Program Studi : KIMIA

Bibliography :
Fardiaz, S. 1983. Mikrobiologi Keamanan Pangan. Bogor: PAU Pangan dan Gizi, Institut Pertanian Bogor. Dalimarta, S. 1999. Atlas Tumbuhan Indonesia. Jilid ke-1. Jakarta: Trubus Agriwidya.

DepKes RI. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: DirJen POM. Erika, B.l. 2000.

Aromex 510, Pemacu Pertumbuhan dan Efeknya Terhadap Kinerja Ayam Broiler. Laporan Penelitian Fakultas Peternakan Institut Pertanian Bogor.

Ganiswara, S.G., et.al. 1995. Farmakologi dan Terapi. Edisi 4. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia.

Gunawan, W.G. 2008. Identifikasi Senyawa Aktif Antibakteri pada Herba Bandotan (Ageratum Conyzoides. Linn).Jurusan Kimia FMIPA Universitas Udayana, Bukit Jimbaran.

Gunawan, P.W. Yulinah, E. Sukrasno Adayana, I.K. (2006). Telaah Antimikroba Daun Babadotan (Ageratum Conyzoides. Linn). African Journal of Pharmaceutica Indonesia.31, (2).

Harbone, J.B. 1975. The Flavonoid. Edisi ke-1. London: Chapman and Hall. Harbone, J.B. 1987. Metode Fitokimia. Diterjemahkan oleh Padmawinata K., Soediro I. Bandung: Institut Teknologi Bandung.

Hutapea J.R. dan Syamsuhidayat S.S. 1991. Inventaris Tanaman Obat Indonesia. Jakarta: Badan Penelitian dan Pengembangan Kesehatan.

Lenny, Sovia .2006. Senyawa Flavonoid, Fenil Propanoida dan Alkaloida. USU Repository. Ming, L.C., 1999. Ageratum conyzoides : A Tropical Source of Medicinal and Agricultural Products. In Janic J. (Ed.). Perspective on New Crops and New Uses. ASHS Press. Virginia, USA.

Pelczar, M.J.Jr. dan Chan, E.C.S. 1986. Dasar-dasar Mikrobiologi. Diterjemahkan oleh Hadioetomo RS, dkk. Jakarta: UI Press. Suradikusumah, E. 1989. Kimia Tumbuhan. Bogor: Pusat Antar Universitas Ilmu Hayati, Institut Pertanian Bogor.

URL : https://journal.unpak.ac.id/index.php/fitofarmaka/article/view/163

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Detail Karya Ilmiah Dosen

Tri Aminingsih, Husain Nashrianto, Aji Syaiful Rohman

Judul	:	POTENSI ANTIBAKTERI TERHADAP Escherichia coli DAN Staphylococcus aureus DAN IDENTIFIKASI SENYAWA EKSTRAK HEKSANA BANDOTAN (Ageratum conyzoides L.) Tri
Abstrak	:	Bandotan (Ageratum conyzoides L.) is a weed plant that is often destroyed, but there is a group of people who use this plant as a traditional medicine that can cure some kinds of diseases such as scab lesions on the skin, malaria, influenza, pneumonia, tumors, and rheumatoid drug. This study aims to identify the substances present in the hexane extract bandotan and test antibacterial activity against Staphylococcus aureus and Escherichia coli. Bandotan herb extracted with hexane using maceration method. Maceration is done in a closed vessel for 24 hours and occasional stirring. Maceration process is performed three times the volume of 500 mL. Hexane extract was concentrated with a rotary evaporator and phytochemical testing includes group alkaloids, saponins, tannins, triterpenoids steroids and flavonoids. Bandotan hexane extract tested antibacterial activity against Staphylococcus aureus and Escherichia coli with a paper disc method and analyzed its chemical compounds by Gas Chromatography-mass spectrophotometry (GC-MS). The results showed that the content of the extract yield 10.01%, 8.41% moisture content, and the hexane extract contains compounds bandotan alkaloids, triterpenoids-steroids and flavonoids. Antibacterial activity with a diameter of inhibition (DDH) against S. aureus and E. coli 29.6 mm 12.4 mm making it more susceptible to S. aureus (gram positive bacteria) compared with E. coli (gram negative bacteria). Component compounds contained in the bandotan hexane extract among others kariofilen, isokariofilen, ageratokromen, demetoksiageratokromen, 6-vinyl-7methoxy-2,2-dimetilkromen, coumarin, dichloroacetic acid, 1-heptadekanol, and 7-ethyl-6methyl 5-metiltiopirazolo [1,5-a] pyrimidine. These compounds are thought to compound that acts as an antibacterial agent. Keywords : Ageratum Conyzoides L., hexane, antibacterial, Escherichia coli, GC-MS Staphylococcus aureus
Tahun	:	2012	Media Publikasi	:	jurnal nasional terakreditasi B
Kategori	:	Jurnal	No/Vol/Tahun	:	1 / 2 / 2012
ISSN/ISBN	:	2087-9164
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	Fardiaz, S. 1983. Mikrobiologi Keamanan Pangan. Bogor: PAU Pangan dan Gizi, Institut Pertanian Bogor. Dalimarta, S. 1999. Atlas Tumbuhan Indonesia. Jilid ke-1. Jakarta: Trubus Agriwidya. DepKes RI. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: DirJen POM. Erika, B.l. 2000. Aromex 510, Pemacu Pertumbuhan dan Efeknya Terhadap Kinerja Ayam Broiler. Laporan Penelitian Fakultas Peternakan Institut Pertanian Bogor. Ganiswara, S.G., et.al. 1995. Farmakologi dan Terapi. Edisi 4. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia. Gunawan, W.G. 2008. Identifikasi Senyawa Aktif Antibakteri pada Herba Bandotan (Ageratum Conyzoides. Linn).Jurusan Kimia FMIPA Universitas Udayana, Bukit Jimbaran. Gunawan, P.W. Yulinah, E. Sukrasno Adayana, I.K. (2006). Telaah Antimikroba Daun Babadotan (Ageratum Conyzoides. Linn). African Journal of Pharmaceutica Indonesia.31, (2). Harbone, J.B. 1975. The Flavonoid. Edisi ke-1. London: Chapman and Hall. Harbone, J.B. 1987. Metode Fitokimia. Diterjemahkan oleh Padmawinata K., Soediro I. Bandung: Institut Teknologi Bandung. Hutapea J.R. dan Syamsuhidayat S.S. 1991. Inventaris Tanaman Obat Indonesia. Jakarta: Badan Penelitian dan Pengembangan Kesehatan. Lenny, Sovia .2006. Senyawa Flavonoid, Fenil Propanoida dan Alkaloida. USU Repository. Ming, L.C., 1999. Ageratum conyzoides : A Tropical Source of Medicinal and Agricultural Products. In Janic J. (Ed.). Perspective on New Crops and New Uses. ASHS Press. Virginia, USA. Pelczar, M.J.Jr. dan Chan, E.C.S. 1986. Dasar-dasar Mikrobiologi. Diterjemahkan oleh Hadioetomo RS, dkk. Jakarta: UI Press. Suradikusumah, E. 1989. Kimia Tumbuhan. Bogor: Pusat Antar Universitas Ilmu Hayati, Institut Pertanian Bogor.
URL	:	https://journal.unpak.ac.id/index.php/fitofarmaka/article/view/163