Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Tuti Susanti Legiastuti, Tri Aminingsih

Judul : Identifikasi Cendawan Endofit Menggunakan Telnik Polymerase Chain Reaction

Abstrak :
Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control strategies of this disease. Isolates of endophytic fungi were collected from chilli pepper growing area in Sleman, Yogyakarta and further identification using molecular technique involving polymerase chain reaction (PCR) and DNA sequencing was performed. DNA fragments of ±500 bp were successfully amplified from 10 fungal isolates by PCR using primer pair ITS1/ITS4, but only 8 DNA sequences was obtained for further genetic analysis. Based on BLASTN analysis the endophytic fungi were identified as having the highest similarity with Pleosporaceae sp. (98%) for H1 isolate, Cercospora nicotianae (100%) for H5 isolate, Cercospora piaropi (98%) for H11 isolate, Guignardia mangiferae (99%) for H16 isolate, Geomyces pannorum (95%) for H17 isolate, Diaporthe phaseoloru (99%) for H18 isolate, Dothideomycete sp. (100%) for K3 isolate, and Alternaria longissima (99%) for K10 isolate. Key words: Begomovirus, chillipepper, DNA sequencing, polymerase chain reaction

Tahun : 2012 Media Publikasi : Jurnal Nasional Terakreditasi A

Kategori : Jurnal No/Vol/Tahun : 2 / 8 / 2012

ISSN/ISBN : 0215-7950

PTN/S : Universitas Pakuan Program Studi : KIMIA

Bibliography :
Alva P, McKenzie EHC, Pointing SB, PenaMuralla R, Hyde KD. 2002. Do sea grasses
35
J Fitopatol Indones Legiastuti dan Aminingsih
harbour endophytes? Fungal Divers Res Series. 7:167-178. Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA. 2003. Fungal endophytes limit pathogen damage in a tropical tree. PNAS USA. 100(26):15649-15654. doi: 10.1073/pnas. 2533483100 Castillo CO, Chalmers KJ, Waugh R, Powell W. 1994. Detection of genetic diversity and selective gene in coffea using RAPD markers. Theor Appl Genet. 87(8):934940. doi: 10.1007/BF00225787. Dingle J, McGee PA. 2003. Some endophytic fungi reduce the density of pustules of Puccinia recondita f.sp. tritici in wheat. Mycol Res. 107(3):310-316. Faeth, SH. 2002. Are endophytic fungi defensive plant mutualists? Oikos. 98(1):25-36. doi:
10.1034/j.1600-0706.2002.980103.x Franzluebbers AJ, Nazih N, Stuedemann JA, Fuhrmann JJ, Schomberg HH, Hartel PG. 1999. Soil carbon and nitrogen poll under low- and high– endophyte infected tall fescue. Soil Sci AM J. 63(6):1687-1694 Frohlich J, Hyde KD, Petrini O. 2000. Endophytic fungi associated with palms. Mycol Res. 104(10):1202-1212. Hernawati H, Wiyono S, Santoso S. 2011. Leaf endophytic fungi of chili (Capsicum annuum) and their role in the protection against Aphis gossypii (Homoptera: Aphididae). Biodiversitas. 12(4):187-191. Hyde KD, Soytong K. 2008. The fungal endophyte dilemma. Fungal Divers. 33:163-173.

Istifadah N, McGee PA. 2006. Endophytic Chaetomium globosum reduces development of tan spot in wheat caused by Pyrenophora triticirepentis. Aus Plant Path. 35(4):411-418. doi.:/10.1071/AP06 038 Lehtonen PT, Helander M, Siddiqui SA, Lehto K, Saikkonen K. 2006. Endophytic fungus decreases plant virus infections in meadow ryegrass (Lolium pratense). Biol Lett. 2(4):620-623. doi: 10.1098/rsbl. 2006.0499. Li WC, Zhou J, Guo SY, Guo LD. 2007. Endophytic fungi associated with lichens in Baihua mountain of Beijing China. Fungal Divers. 25:69-80. Oses R, Valenzuela S, Freer J, Sanfuentes E, Rodriguez J. 2008. Fungal endophytes in xylem of healthy chilean trees and their possible role in early wood decay. Fungal Divers. 33:77-86. Rodrigues KF. 1994. The foliar fungal endophytes of the Amazonian palm Euterpe oleracea. Mycologia. 86(3):376-385. Sambrook J, Russel DW. 2001. Molecular Cloning a Laboratory Manual. Ed ke-3. New York (US): Cold Spring Harbor Laboratory Pr. Suryanarayanan TS, Thirunavukkarasu N, Govindarajulu MB, Sasse F, Jansen R, Murali TS. 2009. Fungal endophytes and bioprospecting. Fungal Biol Rev. 23(12):9-19. doi: 10.1016/j.fbr.2009.07.001 Zabalgogeazcoa I. 2008. Fungal endophytes and their interaction with plant pathogens. Spanish J Agric Res. 6:138-146.

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Detail Karya Ilmiah Dosen

Tuti Susanti Legiastuti, Tri Aminingsih

Judul	:	Identifikasi Cendawan Endofit Menggunakan Telnik Polymerase Chain Reaction
Abstrak	:	Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control strategies of this disease. Isolates of endophytic fungi were collected from chilli pepper growing area in Sleman, Yogyakarta and further identification using molecular technique involving polymerase chain reaction (PCR) and DNA sequencing was performed. DNA fragments of ±500 bp were successfully amplified from 10 fungal isolates by PCR using primer pair ITS1/ITS4, but only 8 DNA sequences was obtained for further genetic analysis. Based on BLASTN analysis the endophytic fungi were identified as having the highest similarity with Pleosporaceae sp. (98%) for H1 isolate, Cercospora nicotianae (100%) for H5 isolate, Cercospora piaropi (98%) for H11 isolate, Guignardia mangiferae (99%) for H16 isolate, Geomyces pannorum (95%) for H17 isolate, Diaporthe phaseoloru (99%) for H18 isolate, Dothideomycete sp. (100%) for K3 isolate, and Alternaria longissima (99%) for K10 isolate. Key words: Begomovirus, chillipepper, DNA sequencing, polymerase chain reaction
Tahun	:	2012	Media Publikasi	:	Jurnal Nasional Terakreditasi A
Kategori	:	Jurnal	No/Vol/Tahun	:	2 / 8 / 2012
ISSN/ISBN	:	0215-7950
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	Alva P, McKenzie EHC, Pointing SB, PenaMuralla R, Hyde KD. 2002. Do sea grasses 35 J Fitopatol Indones Legiastuti dan Aminingsih harbour endophytes? Fungal Divers Res Series. 7:167-178. Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA. 2003. Fungal endophytes limit pathogen damage in a tropical tree. PNAS USA. 100(26):15649-15654. doi: 10.1073/pnas. 2533483100 Castillo CO, Chalmers KJ, Waugh R, Powell W. 1994. Detection of genetic diversity and selective gene in coffea using RAPD markers. Theor Appl Genet. 87(8):934940. doi: 10.1007/BF00225787. Dingle J, McGee PA. 2003. Some endophytic fungi reduce the density of pustules of Puccinia recondita f.sp. tritici in wheat. Mycol Res. 107(3):310-316. Faeth, SH. 2002. Are endophytic fungi defensive plant mutualists? Oikos. 98(1):25-36. doi: 10.1034/j.1600-0706.2002.980103.x Franzluebbers AJ, Nazih N, Stuedemann JA, Fuhrmann JJ, Schomberg HH, Hartel PG. 1999. Soil carbon and nitrogen poll under low- and high– endophyte infected tall fescue. Soil Sci AM J. 63(6):1687-1694 Frohlich J, Hyde KD, Petrini O. 2000. Endophytic fungi associated with palms. Mycol Res. 104(10):1202-1212. Hernawati H, Wiyono S, Santoso S. 2011. Leaf endophytic fungi of chili (Capsicum annuum) and their role in the protection against Aphis gossypii (Homoptera: Aphididae). Biodiversitas. 12(4):187-191. Hyde KD, Soytong K. 2008. The fungal endophyte dilemma. Fungal Divers. 33:163-173. Istifadah N, McGee PA. 2006. Endophytic Chaetomium globosum reduces development of tan spot in wheat caused by Pyrenophora triticirepentis. Aus Plant Path. 35(4):411-418. doi.:/10.1071/AP06 038 Lehtonen PT, Helander M, Siddiqui SA, Lehto K, Saikkonen K. 2006. Endophytic fungus decreases plant virus infections in meadow ryegrass (Lolium pratense). Biol Lett. 2(4):620-623. doi: 10.1098/rsbl. 2006.0499. Li WC, Zhou J, Guo SY, Guo LD. 2007. Endophytic fungi associated with lichens in Baihua mountain of Beijing China. Fungal Divers. 25:69-80. Oses R, Valenzuela S, Freer J, Sanfuentes E, Rodriguez J. 2008. Fungal endophytes in xylem of healthy chilean trees and their possible role in early wood decay. Fungal Divers. 33:77-86. Rodrigues KF. 1994. The foliar fungal endophytes of the Amazonian palm Euterpe oleracea. Mycologia. 86(3):376-385. Sambrook J, Russel DW. 2001. Molecular Cloning a Laboratory Manual. Ed ke-3. New York (US): Cold Spring Harbor Laboratory Pr. Suryanarayanan TS, Thirunavukkarasu N, Govindarajulu MB, Sasse F, Jansen R, Murali TS. 2009. Fungal endophytes and bioprospecting. Fungal Biol Rev. 23(12):9-19. doi: 10.1016/j.fbr.2009.07.001 Zabalgogeazcoa I. 2008. Fungal endophytes and their interaction with plant pathogens. Spanish J Agric Res. 6:138-146.
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