Oom Komala, Novi Fajar Utami, Saraswati Darmawan

Judul	:	Antibacterial Activity of Ocimum citridorum leaf ekstraks Against Shigella dysentriae
Abstrak	:	Dysentery is a disease caused by infection with Shigella dysentriae, one type of acute diarrhea can be seen from the liquid stool mixed with blood and mucus caused by these bacteria. Ocimum citriodorum leaves contain various chemical compounds that can inhibit the growth of bacteriae that cause diarrhea. This study aims to determine the antibacterial activity of O. citriodorum leaves against S. dysentriae by stratified extraction. O. citriodorum leaves were extracted using n-hexane, ethyl acetate, ethanol 96% as solvent. Testing the Minimum Inhibitory Concentration (MIC) on bacteriae at a concentration of 2.5, 5, 10, 15% using the agar dilution method. Inhibition Width (IZW) at a concentration of 15, 25, 30% using the disc paper diffusion method. The positive control used was ciprofloxacin against S. dysentriae bacteria and the negative control used was DMSO 10%. The results showed that the extract of n-hexane, ethyl acetate and ethanol 96% of O. citriodorum leaves had antibacterial activity against S. dysentriae. The most effective MIC of n-hexane, ethyl acetate, ethanol extract 96% of basil leaves was found at a concentration of 15%. IZW ethanol 96% extract of O. citriodorum leaves has most effective antibacterial activity among other solvents at a concentration of 30 % with an IZW value of 9.83 mm against S. dysentriae bacteria.
Tahun	:	2023	Media Publikasi	:	Jurnal Nasional Terakreditasi B
Kategori	:	Jurnal	No/Vol/Tahun	:	1 / 13 / 2023
ISSN/ISBN	:	p-ISSN : 2087-9164/e-ISSN : 2622-755X
PTN/S	:	Universitas Pakuan	Program Studi	:	FARMASI
Bibliography	:	Aini, H. (2017). Pengaruh Pemberian Ekstrak Etanol Daun Kemangi (Ocimum citriodorum) Terhadap Pertumbuhan Bakteri Shigella disentriae; Media of medical laboratory science, 1 (1), Pharmacy Medical Polytechnic of Husada Mataram. https://scholar.google.co.id/citations?user=zJTyGJEAAAAJ&hl=id   Ajizah, A. (2004). Sensitivitas Salmonella typhirmurium terhadap ekstrak daun Psidium guajava l. Biocientie; 1(1): 31-8. http://eprints.ums.ac.id/16342/17/DAFTAR_PUSTAKA_Puri.pdf Bakhtra, D.D.A., Junuary, J., Yusdi, E. (2018). Uji Aktivitas Fraksi Dari Ekstrak Daun Sambung Nyawa (Gynura procumbens (Lour)Merr.) Terhadap Bakteri Shigella dysenteriae: Jurnal farmasi higea, 1(1), College of Pharmacy; Padang. https://www.jurnalfarmasihigea.org/index.php/higea/article/view/175   Balouiri, M., Sadiki, M., & Ibnsouda, S,K. (2016). Methods in vitro evaluating antimicrobial activity: A review. J Pharm Anal, 6, 71.  DOI: 10.1016/j.jpha.2015.11.005.       https://www.ncbi.nlm.nih.gov/pubmed/29403965 Bintang, A. (2018). Perbandingan Metode Ekstraksi Maserasi dan Sokletasi Pada Daun Sirih Hijau (Piper betle) dan Daun Sirih Merah (Piper crocatum Ruiz and pav) Terhadap Candida albicans. Pakuan University, Bogor.   Farha, M.A,  El-Halfawy, O.M.,   Gale, R.T.,  MacNair, C.R.,  Carfrae, L.A.,  Zhang, X., Jentsch, N.G., Magolan, J., Eric, D., & Brown, E.D. (2019). Uncovering the hidden antibiotic potential of Cannabis. bioRxivorg.  DOI: https://doi.org/10.1101/833392       https://www.biorxiv.org/content/10.1101/833392v1 Flanagan, J.N., & Steck, T.R. (2017). The Relationship Between Agar Thickness and Antimicrobial Susceptibility Testing. Indian J Microbiol, 57(4):503–506.  DOI: 10.1007/s12088-017-0683-z       https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671438/   Khalil, A. (2013). Antimicrobial activity of ethanolic extracts of Ocimmum basilicum leaf from Saudi arabia. J Biotechnology. 1-4. https://onesearch.id/Author/Home?author=Khalil%2C+Khalil Madduluri, S., Rao, K.B., & Sitaram, B. (2013). In vitro evaluation of antibacterial activity of five indigenous plants extract against five bacterial pathogen of human. International Journal of Pharmacy and Pharmaceutical Science; 5(4). H. 679-84. https://www.scirp.org/(S(351jmbntvnsjt1aadkposzje))/reference/ReferencesPapers.aspx?ReferenceID=1266694 Margaretta, S., Handayani, Indraswati. N., & Hindraso, H. (2011). Ekstraksi senyawa phenolics Pandanus amaryllifolius Roxb. Sebagai antioksidan alami. Widya teknik. 10(1):21-30. https://media.neliti.com/media/publications/231887-ekstraksi-senyawa-phenolic-pandanus-amar-1b6d7e6c.pdf Munfati, P. N., Ratnasari, E., & Trimulyo, G. (2015). Aktivitas Senyawa Antibakteri Ekstrak Herba Meniran (Phyllanthus niruri) Terhadap Pertumbuhan Bakteri Shigella dysenteriae Secara In Vitro. LenteraBio, 4, (1), 64-65. Faculty of Mathematics and Natural Sciences, State University of Surabaya. https://jurnalmahasiswa.unesa.ac.id/index.php/lenterabio/article/view/10891   Ngajow, M., Abidjulu, J., & Kamu, V.S. (2013). Pengaruh antibakteri ekstrak etanol daun jarak pagar (Jatropha curcas l.) terhadap bakteri Staphylococcus aureus ATCC 25293, Escherichia coli ATCC25922, dan Salmonella typhi ATCC 1408. Jurnal Ilmu Pertanian; 5(2). H. 26-37. https://repository.unair.ac.id/108821/11/11.%20DAFTAR%20PUSTAKA%20.pdf Nur, S., Baitanu, J.A., Gani, S.A. (2019). Pengaruh Tempat Tumbuh Dan Lama Penyulingan Secara Hidrodestilasi Terhadap Rendemen Dan Profil Kandungan Kimia Minyak Atsiri Daun Kemangi (Ocimum canum Sims L). JFFI, 6(2) 363-367. Department of Chemistry, College of Pharmacy, Makassar. https://jurnal.fp.uns.ac.id/index.php/semnas/article/view/1765   Nuzul, P., Daniel, L., & Sepriyanto, D. (2018). Uji Aktivitas Antibakteri Alga Coklat Jenis Padina sp. Dari Pantai Sorido Biak Terhadap Bakteri Staphylococcus aureus Dan Shygella dysentriae. Pharmachy medical journal; pharmacy study program of Cendrawasih University; Jayapura-papua. https://ejournal.unsrat.ac.id/index.php/pmj/article/view/19647/0 Permadi, A., Susanto, Wardatun, S. (2015). Perbandingan Metode Ekstraksi Bertingkat dan Tidak Bertingkat terhadap Flavonoid Total Herba Ciplukan (Phtsalis angulata L.) secara Kolometri: 1(1) : 8. Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Pakuan University. https://jom.unpak.ac.id/index.php/farmasi/article/view/706   Poeloengan, M., & Praptiwi, P. (2012). Uji aktivitas antibakteri ekstrak kulit buah manggis (Garcinia mangostana Linn). Media Litbang Kesehatan; 20(2). H. 65-9. https://media.neliti.com/media/publications/153505-ID-uji-aktivitas-antibakteri-ekstrak-kulit.pdf Radji, M. (2016). Mekanisme aksi molekuler Antibiotik dan Kemoterapi.Penerbit Buku Kedokteran EGC: Jakarta, Hal. 2,10,103. https://opac.perpusnas.go.id/DetailOpac.aspx?id=1061807 Sukadana, I.M. (2009). Senyawa Antibakteri Golongan Flavonoid dari Buah Belimbing Manis (Averrhoa carambola Linn.L). Jurnal Kimia. 3 (2): 109 116. https://ojs.unud.ac.id/index.php/jchem/article/view/2740 Tortora, G.J., Funke, & C.L. Case. (2010). Microbiology an introduction San Fransissco.USA: Addison Wesley Longman Inc. https://www.scirp.org/(S(lz5mqp453ed%20snp55rrgjct55))/reference/referencespapers.aspx?referenceid=2992027 W.H.O. (2016). Dysenteriae (Shigellosis). Accessed Tuesday, 6 June 2017. https://www.who.int/teams/immunization-vaccines-and-biologicals/diseases/shigella   Wulansari, Y., Suswati, E., & Wahyudi, S.W. (2018). Uji In Vitro Aktivitas Antibakteri Minyak Atsiri Batang Sereh (Cymbopogon citratus) Terhadap Shigella dysentriae; e-jurnal Pustaka Kesehatan, 6(2), Faculty of Medicine, University of Jember. https://jurnal.unej.ac.id/index.php/JPK/article/view/7665
URL	:	journal.unpak.ac.id