Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Lusi Indriani, Moerfiah, Oktaviana Zunnita, Faisal Pradana

Judul : POTENSI ANTIPLATELET CAMPURAN EKSTRAK BINAHONG, JAHE DAN KUNYIT PADA MENCIT PUTIH JANTAN

Abstrak :
Antiplatelets can inhibit platelets aggregation and have been used in prevention and treatment the trombotic desease. Binahong leaves, ginger dan turmeric have antiplatelets activity. The mixture of these plants are expected to increase the antiplatelets potency. This study aimed to determine the optimum dose of ethanol extracts of binahong leaves, ginger dan turmeric as antiplatelets agent. The antiplatelets testing were carried out by in vivo method using parameter of bleeding and coagulation time. The testing solutions were administered orally once a day for 8 days. There were 7 treatment groups consist of positive control (Acetosal 0.29mg/20gBW), negative control (CMC-Na 0.5%), binahong 10mg/20gBW, mixture 1 of binahong:ginger:turmeric (5:0.5:1mg/20gBW), mixture 2 (10:0.5:1mg/20gBW), mixture 3 (5:1:1mg/20gBW), and mixture 4 (5:0.5:2mg/20gBW). The bleeding and coagulation time were determined at 0, 3rd, 6th and 9th day. The bleeding time was determined by injuring mice tail, the blood that comes out were absorbed with absorption paper. From the first bleeding time until stopped was calculated as bleeding time. The coagulation time was determined by observing the formation of fibrin thread in the broken capillary pipe. The blood from the end tail were absorbed with capillary pipe, and then was broken every 15 seconds. The antiplatelets activity in mice with bleeding time parameter showed that mixture 3 was more effective at 3rd day with an increased of 16.60%, than the positive control group (3.92%). The coagulation time parameter showed that the binahong group was more effective at 9th day with an increased of 203.55%, than the positive control group (89.71%). The mixture of binahong, ginger, and turmeric were the most effective in increasing bleeding time, whereas binahong was the most effective in increasing coagulation time.

Keywords : Binahong ginger turmeric, Antiplatelets, Bleeding Time

Tahun : 2021 Media Publikasi : Jurnal Nasional Terakreditasi B

Kategori : Jurnal No/Vol/Tahun : 2 / 7 / 2021

ISSN/ISBN : 2477-1821

PTN/S : Universitas Pakuan Program Studi : FARMASI

Bibliography :
1. Saraf, F., Benzalha, I., Gorog, D.A. 2009. Antiplatelet Resistence-Does It Exist and How to Measure it. Clinical Medicine Cardiology. 2009; 3: 77-91.

2. Gross PL, Weitz JL. 2009. New Antithrombotic Drugs. Clinical Pharmacology & Therapeutics. 2009; 86 (2): 139-146.

3. Ebadi, M. 2008. Desk Reference Of Clinical Pharmacology. New York: CRC Press.

4. Aprilia, C.A., dan Dewiastuti, M. 2017. Efektivitas Hipolipidemia dan Antioksidan Ekstrak Daun Binahong pada Tikus Putih yang Diinduksi Pakan Hiperkolesterol. Jurnal Kedokteran Yarsi 25 (3) : 150-162.

5. Fitriyani, N., & Sigit, J. I. (2008). Efek antiagegasi platelet ekstrak etanol buah mengkudu (Morinda citrifolia L.), rimpang jahe merah (Zingiber officinale var. Sunti Val.) dan kombinasinya pada mencit jantan galur swiss webster. Maranatha Journal of Medicine and Health, 7(2), 149447.

6. Sukandar, E.Y., Sigit, J.I., Fitriani, Nurul. 2008. Efek antiagregasi platelet ekstrak air bulbus bawang putih (Allium sativum L.), ekstrak etanol rimpang kunyit (Curcuma domestica Val.) dan kombinasinya pada mencit jantan galur Swiss Webster. Majalah Farmasi Indonesia. 2008.19(1):1–11.

7. Jazilah, N. 2014. Uji Toksisitas Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis terhadap Larva Udang Artemia salina Leach dengan Metode Brine Shrimp Lethality Test (BSLT). Skripsi. Jurusan Kimia, Fakultas Sains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim Malang.

8. Wei, B.L., Weng, J.R., Chiu, P.H., Hung, C.F., Wang, J.P., Lin, C.N. 2005. Antiinflammatory Effect of Flavonoids from Artocarpus heterophyllus and Artocarpus communis. J. Agric. Food. Chem. 2005; 53 (10) 3867-3871.

9. Departemen Kesehatan Republik Indonesia. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Kementrian Kesehatan Republik Indonesia: Jakarta.

10. Hanani, E. 2015. Analisis Fitokimia. Jakarta: Penerbit Buku Kedokteran EGC.

11. Putri, R.R.R.F., Ulfa, E.U., dan Riyanti, R. 2014. Uji Aktivitas Antiplatelet Ekstrak Etanol Kubis Merah (Brassica oleracea var. capitata L.). e-Jurnal Pustaka Kesehatan, vol. 2 (no. 1), Januari 2014.

12. Kementrian Kesehatan Republik Indonesia. 2017. Farmakope Herbal Indonesia. Edisi II. Jakarta : Kementrian Kesehatan Republik Indonesia.

13. Departemen Kesehatan Republik Indonesia. 2013. Suplemen III Farmakope Herbal Indonesia, Edisi I. Jakarta.

14. Paskartini, T. G., 2017. Parameter Standarisai Tanaman Segar Simplisia dan Ekstrak Etanol Daun Binahong (Anredera cordifolia) Dari Tiga Daerah Berbeda. Skripsi. Universitas Khatolik Widya Mandala, Surabaya.

15. Agustina, S., Ruslan dan Wiraningtyas, A. 2016. Skrining Fitokimia Tanaman Obat Di Kabupaten Bima. Indonesia EJournal of Applled Chemistry. Vol 4.

16. Jantan, I., Raweh, S.M., Sirat, H.M., Jamil, S., Mohd Yasin, Y.H., Jalil, J., et al. 2008. Inhibitory effect of compounds from Zingiberaceae species on human platelet aggregation. Phytomedicine;15(4):306–9.

17. Hidayah, L. (2016). Efektivitas Ekstrak Kulit Kayu Manis (Cinnamomum burmannii) terhadap Perpanjangan Waktu Perdarahan pada Mencit Jantan Galur Swiss Webster. Jurnal Gizi dan Kesehatan. Vol 8 No.17. Hal 97-102.

18. Middleton, E., Kandaswami, C., & Theoharides, T. C. (2000). The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological reviews, 52(4), 673- 751.

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Detail Karya Ilmiah Dosen

Lusi Indriani, Moerfiah, Oktaviana Zunnita, Faisal Pradana

Judul	:	POTENSI ANTIPLATELET CAMPURAN EKSTRAK BINAHONG, JAHE DAN KUNYIT PADA MENCIT PUTIH JANTAN
Abstrak	:	Antiplatelets can inhibit platelets aggregation and have been used in prevention and treatment the trombotic desease. Binahong leaves, ginger dan turmeric have antiplatelets activity. The mixture of these plants are expected to increase the antiplatelets potency. This study aimed to determine the optimum dose of ethanol extracts of binahong leaves, ginger dan turmeric as antiplatelets agent. The antiplatelets testing were carried out by in vivo method using parameter of bleeding and coagulation time. The testing solutions were administered orally once a day for 8 days. There were 7 treatment groups consist of positive control (Acetosal 0.29mg/20gBW), negative control (CMC-Na 0.5%), binahong 10mg/20gBW, mixture 1 of binahong:ginger:turmeric (5:0.5:1mg/20gBW), mixture 2 (10:0.5:1mg/20gBW), mixture 3 (5:1:1mg/20gBW), and mixture 4 (5:0.5:2mg/20gBW). The bleeding and coagulation time were determined at 0, 3rd, 6th and 9th day. The bleeding time was determined by injuring mice tail, the blood that comes out were absorbed with absorption paper. From the first bleeding time until stopped was calculated as bleeding time. The coagulation time was determined by observing the formation of fibrin thread in the broken capillary pipe. The blood from the end tail were absorbed with capillary pipe, and then was broken every 15 seconds. The antiplatelets activity in mice with bleeding time parameter showed that mixture 3 was more effective at 3rd day with an increased of 16.60%, than the positive control group (3.92%). The coagulation time parameter showed that the binahong group was more effective at 9th day with an increased of 203.55%, than the positive control group (89.71%). The mixture of binahong, ginger, and turmeric were the most effective in increasing bleeding time, whereas binahong was the most effective in increasing coagulation time. Keywords : Binahong ginger turmeric, Antiplatelets, Bleeding Time
Tahun	:	2021	Media Publikasi	:	Jurnal Nasional Terakreditasi B
Kategori	:	Jurnal	No/Vol/Tahun	:	2 / 7 / 2021
ISSN/ISBN	:	2477-1821
PTN/S	:	Universitas Pakuan	Program Studi	:	FARMASI
Bibliography	:	1. Saraf, F., Benzalha, I., Gorog, D.A. 2009. Antiplatelet Resistence-Does It Exist and How to Measure it. Clinical Medicine Cardiology. 2009; 3: 77-91. 2. Gross PL, Weitz JL. 2009. New Antithrombotic Drugs. Clinical Pharmacology & Therapeutics. 2009; 86 (2): 139-146. 3. Ebadi, M. 2008. Desk Reference Of Clinical Pharmacology. New York: CRC Press. 4. Aprilia, C.A., dan Dewiastuti, M. 2017. Efektivitas Hipolipidemia dan Antioksidan Ekstrak Daun Binahong pada Tikus Putih yang Diinduksi Pakan Hiperkolesterol. Jurnal Kedokteran Yarsi 25 (3) : 150-162. 5. Fitriyani, N., & Sigit, J. I. (2008). Efek antiagegasi platelet ekstrak etanol buah mengkudu (Morinda citrifolia L.), rimpang jahe merah (Zingiber officinale var. Sunti Val.) dan kombinasinya pada mencit jantan galur swiss webster. Maranatha Journal of Medicine and Health, 7(2), 149447. 6. Sukandar, E.Y., Sigit, J.I., Fitriani, Nurul. 2008. Efek antiagregasi platelet ekstrak air bulbus bawang putih (Allium sativum L.), ekstrak etanol rimpang kunyit (Curcuma domestica Val.) dan kombinasinya pada mencit jantan galur Swiss Webster. Majalah Farmasi Indonesia. 2008.19(1):1–11. 7. Jazilah, N. 2014. Uji Toksisitas Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis terhadap Larva Udang Artemia salina Leach dengan Metode Brine Shrimp Lethality Test (BSLT). Skripsi. Jurusan Kimia, Fakultas Sains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim Malang. 8. Wei, B.L., Weng, J.R., Chiu, P.H., Hung, C.F., Wang, J.P., Lin, C.N. 2005. Antiinflammatory Effect of Flavonoids from Artocarpus heterophyllus and Artocarpus communis. J. Agric. Food. Chem. 2005; 53 (10) 3867-3871. 9. Departemen Kesehatan Republik Indonesia. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Kementrian Kesehatan Republik Indonesia: Jakarta. 10. Hanani, E. 2015. Analisis Fitokimia. Jakarta: Penerbit Buku Kedokteran EGC. 11. Putri, R.R.R.F., Ulfa, E.U., dan Riyanti, R. 2014. Uji Aktivitas Antiplatelet Ekstrak Etanol Kubis Merah (Brassica oleracea var. capitata L.). e-Jurnal Pustaka Kesehatan, vol. 2 (no. 1), Januari 2014. 12. Kementrian Kesehatan Republik Indonesia. 2017. Farmakope Herbal Indonesia. Edisi II. Jakarta : Kementrian Kesehatan Republik Indonesia. 13. Departemen Kesehatan Republik Indonesia. 2013. Suplemen III Farmakope Herbal Indonesia, Edisi I. Jakarta. 14. Paskartini, T. G., 2017. Parameter Standarisai Tanaman Segar Simplisia dan Ekstrak Etanol Daun Binahong (Anredera cordifolia) Dari Tiga Daerah Berbeda. Skripsi. Universitas Khatolik Widya Mandala, Surabaya. 15. Agustina, S., Ruslan dan Wiraningtyas, A. 2016. Skrining Fitokimia Tanaman Obat Di Kabupaten Bima. Indonesia EJournal of Applled Chemistry. Vol 4. 16. Jantan, I., Raweh, S.M., Sirat, H.M., Jamil, S., Mohd Yasin, Y.H., Jalil, J., et al. 2008. Inhibitory effect of compounds from Zingiberaceae species on human platelet aggregation. Phytomedicine;15(4):306–9. 17. Hidayah, L. (2016). Efektivitas Ekstrak Kulit Kayu Manis (Cinnamomum burmannii) terhadap Perpanjangan Waktu Perdarahan pada Mencit Jantan Galur Swiss Webster. Jurnal Gizi dan Kesehatan. Vol 8 No.17. Hal 97-102. 18. Middleton, E., Kandaswami, C., & Theoharides, T. C. (2000). The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological reviews, 52(4), 673- 751.
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