Ade Heri Mulyati, Siti Warnasih, Sutanto

Judul	:	Determination of the Aflatoxin levels in Corn (Zea mays, L.) during storage process
Abstrak	:	Abstract. Aflatoxin contaminant in corn being a serious problem, due to its toxicity. This contamination was influenced by the storage and treatment process. During storage, the aflatoxin level was influenced by the water content of corn seed. In this study, the water content were performed by gravimetric method, while the aflatoxin contents have been carried out by ELISA and HPLC methods. The corn seed samples were obtained from Tangerang, Cikande, and Cirebon. The results showed that the aflatoxin content were increased in fourthweek storage.The analysis of the aflatoxin content using the ELISA method showed that corn seed obtained from Tangerang, Cikande, and Cirebon were 74,06 ppb; 9.33 ppb; 28.67 ppb respectively, while the HPLC method showed that the aflatoxin content were 75.02 ppb; 9.83 ppb; 28.43ppb respectively. The results also showed that the water content in the corn seed obtained from Tangerang, Cikande, and Cirebon were 10.64%; 10.17%, 10.25% respectively on fourth-week storage. In conclusion, the aflatoxin content in corn seed from Cikande and Cirebon were in accordance with SNI standards, while corn seed from Tangerang contain aflatoxin was above 50 ppb.  Keywords: corn seed, aflatoxin, water content, ELISA, HPLC
Tahun	:	2021	Media Publikasi	:	Jurnal Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	Journal of Physics: Conference Series 1882 (2021) 012111 / doi:10.1088/1742-6596/1882/1/012111 / 2021
ISSN/ISBN	:	doi:10.1088/1742-6596/1882/1/012111
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	6. References  [1]  Miskiyah M and Widaningrum W 2013 Pengendalian aflatoksin pada pascapanen jagung melalui penerapan HACCP Jurnal Standardisasi 10 1 1 https://doi.org/10.31153/js.v10i1.2  [2]  Warburton M L and Williams W P 2014 Aflatoxin Resistance in Maize: What Have We Learned Lately? Advances in Botany 2014 1–10. https://doi.org/10.1155/2014/352831  [3] Yu, J, Hennessy D A and Wu F 2020 The Impact of Bt Corn on Aflatoxin-Related Insurance Claims in the United States Scientific Reports 10 1 1–10 https://doi.org/10.1038/s41598-020-66955-1 [4]  Tandiabang J 2011 Kajian Pengendalian Aflatoksin Pada Jagung Seminar Nasional Serealia  419–425 [5]  Serumaga J P, Ortega-Beltran A, Wagacha J M, Mutegi C K and Bandyopadhyay R 2020  Aflatoxin-producing fungi associated with pre-harvest maize contamination in Uganda International Journal of Food Microbiology 313 September 2019 108376 https://doi.org/10.1016/j.ijfoodmicro.2019.108376  [6]  Sukmawati D, Wahyudi P, Rahayu S, Moersilah M, Handayani T, Rustam K Y and Puspitasari S I 2018 Skrining Kapang Aspergillus Spp. Penghasil Aflatoksin Pada Jagung Pipilan Di Daerah Bekasi, Jawa Barat Al-Kauniyah: Jurnal Biologi 11 2 151–162 https://doi.org/10.15408/kauniyah.v11i2.6961  [7]  Piedade F S, Fonseca H, Glória E M, Calori-Domingues M A, Piedade S M S and Barbin D 2002 Distribution of aflatoxins in contaminated corn fractions segregated by size Brazilian Journal of Microbiology 33 1 12–16 https://doi.org/10.1590/S1517-83822002000100002  [8]  Kusumaningrum H D, Toha A D, Putra S H and Utami A S 2010 Contamination of Aspergillus flavus and Aflatoxin at Distribution Chain of Maize Based Food Product and its Influencing Factors Jurnal Teknologi Dan Industri Pangan  XXI 2 171–176  [9]  Widiastuti R 2006 Mycotoxins: Effect on Animal Health and Residues in Animal Products and Their Control Wartazoa 16 3 116-127  [10]  Kumar P, Mahato D K, Kamle M, Mohanta T K and Kang S G 2017 Aflatoxins: A global concern for food safety, human health and their management Frontiers in Microbiology 7 JAN 1–10 https://doi.org/10.3389/fmicb.2016.02170  [11]  Tao F, Yao H, Hruska Z, Liu Y, Rajasekaran K. and Bhatnagar D 2020 Detection of aflatoxin B1 on corn kernel surfaces using visible-near infrared spectroscopy Journal of Near Infrared Spectroscopy 28 2 59–69 https://doi.org/10.1177/0967033519895686  [12]  Samuel S M 2014 Analytical Methods In Determining Aflatoxin B1 In Food Materials International Journal of Advance Research April 2013 1–11  [13]  Munkvold G et al. 2002 Aflatoxin in Corn (Iowa State University, University Extension) [14]  Warburton M L and Williams W P 2014 Aflatoxin Resistance in Maize: What Have We  Learned Lately? Advances in Botany 2014 1–10 https://doi.org/10.1155/2014/352831 [15]  Kasno A 2004 Prevention of Aspergillus flavus Infection and Aflatoxin Contamination in Peanuts J. Agricultural Research and Development 23 3 75 - 81 [16]  Barbas C, Dams A, Majors R E 2005 Separation of Aflatoxin by HPLC (USA: Agilent Tech  Application. Inc.) [17]  Garcia-Villanova R J, Cordon C, Paramas A M G, Aparicio P and Rosales M E G 2004 Simultaneous Immunoaffinity Column Clean Up and HOLC Analysis of Aflatoxin and Ochratoxin A in Spanish Bee Pollen J. of Agric. & Food Chemist 52 24 7235-7239
URL	:	doi:10.1088/1742-6596/1882/1/012111