Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

M Amin, M F Yusuf, E Suarsini, F A Anugrah, E W Alita, I Rachman, T Matsumoto

Judul : Identification of indigene bacteria from waste water of Regional Public Hospitals in Pacitan

Abstrak :
Hospital wastes has a huge impact and harmful to the living creatures around the disposal area, although proper treatment has been performed by using an appropriate waste water treatment plant (WWTP). Ineffective use of an integrated WWTP on wastewater is not be able to fulfill the environmental constitution. It was required more efficient and eco-friendly invention of bioremediation by using indigenic bacteria. The purpose of this study was to identify bacteria species and characteristic of bacteria candidates potentially as bioremediation agent. Indigenic bacteria are obtained from wastes of Dr. Darsono Hospital in Pacitan Regency, which becoming a source of organic nutritions (amylum, protein and lypid) for indigene bacteria. The potency of indigene bacteria in decomposing wastes by degrading organic pollutant substances waste can be determined by calculating the hydrolysis index result. In sequence, four species of bacteria potentially as candidates for bioremediation are Acinetobacter baumannii, Enterobacter agglomerans, Aeromonas hydrophila, and Burkholderia cepacia. There were three isolates classified as amylolytic bacteria (Acinetobacter baumannii, Enterobacter agglomerans, and Aeromonas hydrophila), Aeromonas hydrophilla clasified as proteolytic bacteria and two isolates as lipolytic bacteria (Burkholderia cepacia dan Aeromonas hydrophilla). Enterobacter agglomerans having the highest hydrolysis of amylum with amylolytic index = 2.94, Aeromonas hydrophila has the highest protein hydrolysis with proteolytic index = 7.80, and Burkholderia cepacia has the highest lypid with hydrolysis index = 7.00.

Tahun : 2021 Media Publikasi : Prosiding

Kategori : Prosiding No/Vol/Tahun : 012089 / 230 / 2019

ISSN/ISBN : -

PTN/S : Universitas Negeri Malang, University of Kitakyushu Program Studi : PENDIDIKAN ILMU PENGETAHUAN ALAM

Bibliography :
[1] Undang-Undang Republik Indonesia Nomor 44 Tahun 2009 Tentang Rumah Sakit Pasal 11 (Regulation No. 44 Year 2009 about Hospital article 11). [In Indonesian] [2] Departemen Perindustrian 2012 Cleaner Producion: Pengelolaan Limbah Industri Pangan (Cleaner Production: Food Industry Waste Management] Department of Trade Indonesia. [In Indonesian] [3] Lade H, Kadam A, Paul D, Govindwar S 2015 Biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes EXCLI J 14 158-174. [4] Thompson I P, Gast C J V D, Ciric L, Singer A C 2005 Bioaugmentation for bioremediation the challenge of strain selection Environ. Microbiol.7 7 909-915. [5] Bergey D H, Buchanan R E, Gibbons N E 1974 Bergey’s Manual of Determinative Bacteriology 8th Ed. Williams and Wilkins Co Baltimore USA. [6] Melliawati R, Djohan A C, Yopi 2015 Seleksi bakteri asam laktat sebagai penghasil enzim protease (Selection of lactic acid bacteria as a producer of the protease enzyme) Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia 1 2 184-188. [In Indonesian] [7] Wahyudi P, Rachmania R A, Ramdham M, Sari N, Nuriam M S, Hardi D, Purwanti T 2014 Isolasi bakteri amilolitik dan optimasi kondisi fermentasi untuk produksi enzim α-amilase (Isolation of amylolytic bacteria and optimization of fermentation conditions for the production of α-amylase enzymes) FARMASAINS 2 3 1-8. [In Indonesian] [8] Pakpahan R 2009 Isolasi Bakteri dan Uji Aktivitas Protease Termofilik dari Sumber Air Panas Sipoholon Tapanuli Utara Sumatera Utara (Bacterial Isolation and Thermophilic Protease Activity Test from Sipoholon North Tapanuli Hot Springs North Sumatera) Master Thesis Universitas Sumatera Utara Indonesia. [In Indonesian] [9] Lee L P, Karbul H M, Citartan M, Gopinath S C B, Lakshmipriya T, Tang T H 2015 Lipase secreting Bacillus species in an oil-contaminated habitat: promising strains to alleviate oil pollution BioMed Res. Int.2015 1-9. [10] Loehfelm T W, Luke N R, Campagnari A A 2008 Identification and characterization of an Acinetobacter baumannii biofilm-associated protein J. Bacteriol. 190 3 1036-1044. [11] Pascon R C, Bergamo R F, Spinelli R X, de Souza E D, Assis D M, Juliano L, Vallim M A 2011 Amylolytic microorganism from São Paulo Zoo composting: isolation, identification, and amylase production Enzyme Res. 2011 1-8. [12] Onishi H, Hidaka O 1978 Purification and properties of amylase produced by a moderately halophilic Acinetobacter sp. Can. J Microbiol. 24 10 17–23. [13] Erdem B, Kariptas E, Cil E, Isik K 2011Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey Turk. J. Biol. 35 463-472.

[14] Aldeen W R T, Abeer F A, Oruba K H, Al-bermani N S N 2014 PCR detection of putative hemolysin and aerolysin genes in an Aeromonas hydrophila Isolates from Diarrhea in Babylon Province. J. Natur. Sci. Res. 11 4 41-47. [15] Wise M G, Shimkets L J, McArthur J V 1995 Genetic structure of a lotic population of Burkholderia (Pseudomonas) cepacia Appl. Environ. Microbiol. 61 1791-1798. [16] Galbraith L, Martina H J, Charlotta R, Stephen G W 1998 Lipids and fatty acids of Burkholderia and Ralstonia species FEMS Microbiol. Lett. 173 2 359-364. [17] Stead D E, Sellwood J E, Wilson J, Viney I 1992 Evaluation of a commercial microbial identification system based on fatty acid pro¢les for rapid, accurate identification of plant pathogenic bacteria J. Appl. Bacteriol. 72 315-321. [18] Shotss E B, Tsu T C, Waltman W T 1984 Extracelullar proteolytic activity Aeeromonas hydrophila complex. Georgia. Fish Pathology 20 1 37-44. [19] Merino S, Markus W, Juan M T 2014 Role of Aeromonas hydrophila flagella glycosylation in adhesion to hep-2 cells, biofilm formation and immune stimulation Int. J. Mol. Sci. 15 21935- 21946. [20] Yousry M G, El-Naggar M M, Soliman M K, Barakat K M 2010 Characterization of marine Burkholderia cepacia antibacterial agents. J. Natur. Prod. 3 86-94. [21] Gaman P M, Sherrington K B 1981 The science of food: An introduction to food science, nutrition, and microbiology Infect control Hosp Epidemiol 16 30-32.

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Detail Karya Ilmiah Dosen

M Amin, M F Yusuf, E Suarsini, F A Anugrah, E W Alita, I Rachman, T Matsumoto

Judul	:	Identification of indigene bacteria from waste water of Regional Public Hospitals in Pacitan
Abstrak	:	Hospital wastes has a huge impact and harmful to the living creatures around the disposal area, although proper treatment has been performed by using an appropriate waste water treatment plant (WWTP). Ineffective use of an integrated WWTP on wastewater is not be able to fulfill the environmental constitution. It was required more efficient and eco-friendly invention of bioremediation by using indigenic bacteria. The purpose of this study was to identify bacteria species and characteristic of bacteria candidates potentially as bioremediation agent. Indigenic bacteria are obtained from wastes of Dr. Darsono Hospital in Pacitan Regency, which becoming a source of organic nutritions (amylum, protein and lypid) for indigene bacteria. The potency of indigene bacteria in decomposing wastes by degrading organic pollutant substances waste can be determined by calculating the hydrolysis index result. In sequence, four species of bacteria potentially as candidates for bioremediation are Acinetobacter baumannii, Enterobacter agglomerans, Aeromonas hydrophila, and Burkholderia cepacia. There were three isolates classified as amylolytic bacteria (Acinetobacter baumannii, Enterobacter agglomerans, and Aeromonas hydrophila), Aeromonas hydrophilla clasified as proteolytic bacteria and two isolates as lipolytic bacteria (Burkholderia cepacia dan Aeromonas hydrophilla). Enterobacter agglomerans having the highest hydrolysis of amylum with amylolytic index = 2.94, Aeromonas hydrophila has the highest protein hydrolysis with proteolytic index = 7.80, and Burkholderia cepacia has the highest lypid with hydrolysis index = 7.00.
Tahun	:	2021	Media Publikasi	:	Prosiding
Kategori	:	Prosiding	No/Vol/Tahun	:	012089 / 230 / 2019
ISSN/ISBN	:	-
PTN/S	:	Universitas Negeri Malang, University of Kitakyushu	Program Studi	:	PENDIDIKAN ILMU PENGETAHUAN ALAM
Bibliography	:	[1] Undang-Undang Republik Indonesia Nomor 44 Tahun 2009 Tentang Rumah Sakit Pasal 11 (Regulation No. 44 Year 2009 about Hospital article 11). [In Indonesian] [2] Departemen Perindustrian 2012 Cleaner Producion: Pengelolaan Limbah Industri Pangan (Cleaner Production: Food Industry Waste Management] Department of Trade Indonesia. [In Indonesian] [3] Lade H, Kadam A, Paul D, Govindwar S 2015 Biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes EXCLI J 14 158-174. [4] Thompson I P, Gast C J V D, Ciric L, Singer A C 2005 Bioaugmentation for bioremediation the challenge of strain selection Environ. Microbiol.7 7 909-915. [5] Bergey D H, Buchanan R E, Gibbons N E 1974 Bergey’s Manual of Determinative Bacteriology 8th Ed. Williams and Wilkins Co Baltimore USA. [6] Melliawati R, Djohan A C, Yopi 2015 Seleksi bakteri asam laktat sebagai penghasil enzim protease (Selection of lactic acid bacteria as a producer of the protease enzyme) Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia 1 2 184-188. [In Indonesian] [7] Wahyudi P, Rachmania R A, Ramdham M, Sari N, Nuriam M S, Hardi D, Purwanti T 2014 Isolasi bakteri amilolitik dan optimasi kondisi fermentasi untuk produksi enzim α-amilase (Isolation of amylolytic bacteria and optimization of fermentation conditions for the production of α-amylase enzymes) FARMASAINS 2 3 1-8. [In Indonesian] [8] Pakpahan R 2009 Isolasi Bakteri dan Uji Aktivitas Protease Termofilik dari Sumber Air Panas Sipoholon Tapanuli Utara Sumatera Utara (Bacterial Isolation and Thermophilic Protease Activity Test from Sipoholon North Tapanuli Hot Springs North Sumatera) Master Thesis Universitas Sumatera Utara Indonesia. [In Indonesian] [9] Lee L P, Karbul H M, Citartan M, Gopinath S C B, Lakshmipriya T, Tang T H 2015 Lipase secreting Bacillus species in an oil-contaminated habitat: promising strains to alleviate oil pollution BioMed Res. Int.2015 1-9. [10] Loehfelm T W, Luke N R, Campagnari A A 2008 Identification and characterization of an Acinetobacter baumannii biofilm-associated protein J. Bacteriol. 190 3 1036-1044. [11] Pascon R C, Bergamo R F, Spinelli R X, de Souza E D, Assis D M, Juliano L, Vallim M A 2011 Amylolytic microorganism from São Paulo Zoo composting: isolation, identification, and amylase production Enzyme Res. 2011 1-8. [12] Onishi H, Hidaka O 1978 Purification and properties of amylase produced by a moderately halophilic Acinetobacter sp. Can. J Microbiol. 24 10 17–23. [13] Erdem B, Kariptas E, Cil E, Isik K 2011Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey Turk. J. Biol. 35 463-472. [14] Aldeen W R T, Abeer F A, Oruba K H, Al-bermani N S N 2014 PCR detection of putative hemolysin and aerolysin genes in an Aeromonas hydrophila Isolates from Diarrhea in Babylon Province. J. Natur. Sci. Res. 11 4 41-47. [15] Wise M G, Shimkets L J, McArthur J V 1995 Genetic structure of a lotic population of Burkholderia (Pseudomonas) cepacia Appl. Environ. Microbiol. 61 1791-1798. [16] Galbraith L, Martina H J, Charlotta R, Stephen G W 1998 Lipids and fatty acids of Burkholderia and Ralstonia species FEMS Microbiol. Lett. 173 2 359-364. [17] Stead D E, Sellwood J E, Wilson J, Viney I 1992 Evaluation of a commercial microbial identification system based on fatty acid pro¢les for rapid, accurate identification of plant pathogenic bacteria J. Appl. Bacteriol. 72 315-321. [18] Shotss E B, Tsu T C, Waltman W T 1984 Extracelullar proteolytic activity Aeeromonas hydrophila complex. Georgia. Fish Pathology 20 1 37-44. [19] Merino S, Markus W, Juan M T 2014 Role of Aeromonas hydrophila flagella glycosylation in adhesion to hep-2 cells, biofilm formation and immune stimulation Int. J. Mol. Sci. 15 21935- 21946. [20] Yousry M G, El-Naggar M M, Soliman M K, Barakat K M 2010 Characterization of marine Burkholderia cepacia antibacterial agents. J. Natur. Prod. 3 86-94. [21] Gaman P M, Sherrington K B 1981 The science of food: An introduction to food science, nutrition, and microbiology Infect control Hosp Epidemiol 16 30-32.
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