Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Akhmad Endang Zainal Hasan, Nurliani Bermawie, Heddy Julistiono, Eny Ida Riyanti, Hasim Hasim, Usep Suhendar

Judul : PHYTOCHEMICAL SCREENING AND ANTI-BREASTCANCER ACTIVITIES OF ANNONA MURICATA (L.) LEAF EXTRACTS

Abstrak :
The objective of the research is to study antiproliferation activities (anti breastcancer) of leaf extracts soursop (Annona muricata L.) from five locations growth in West Java, Indonesia against breast-cancer cell line Michigan Cancer Foundation-7 (MCF-7). Each of the soursop leaf from five locations, was extracted with 70% ethanol using a microwave as a tool for extraction. In vitro test against breast cancer cells growth was conducted using the MCF-7 cell line by means of the MTT-assay.. Based on the analysis of phytochemicals, all soursop leaf extracts contain alkaloids, triterpenoids, tannins, saponins and flavonoids, but did not contain any steroids compound. Soursop leaf extracts with the highest anticancer activity were derived from Sukabumi 1 with an IC50 value of 12.5 µg/ml. The results obtained from this study indicate the ability of soursop leaf extracts to inhibite the growth of breast cancer cells (MCF-7). Such extracts could be used in the treatment of cancer patients.

Tahun : 2020 Media Publikasi : Jurnal Internasional

Kategori : Jurnal No/Vol/Tahun : 24 / 2 / 2020

ISSN/ISBN : 1827-8620

PTN/S : Universitas Pakuan Program Studi : FARMASI

Bibliography :
1. [WHO] World Health Organization. The Global
Burden of Disease: 2004 Update. 2008. Geneva:
World Health Organization.
htt://www.who.int/healthinfo/global_burden_
disease/GBD_report_2004update_part1.pdf [29
October 2010].
2. [Ministry of Health] Kementerian Kesehatan
Republik Indonesia. 2013.
http://www.depkes.go.id/index.php/berita/pressrelease/2233-seminar-sehari

-dalam-rangkamemperingati-hari-kanker-sedunia-2013.html

[14
June 2013].
3. Solomon-Wisdom GO, Ugoh SC, Muhammad B.
2014. Phytochemical Screening and Antimicrobial
activities of Annona muricata (L) leaf extract. Am
J Biol Chem Pharm Sci.2:1-7.
4. Paarakh PM, JPN Chansouria, RL Khosa. 2009.
Wound Healing Activity of Annona muricata
Extract. J Pharm Res. 2: 404-6.
5. Moghadamtousi SZ, HA Kadir, M Paydar, E
Rouhollai, H Karimian. 2014. Annona muricata
leaf induced apoptosis in A549 cells through
mitochondrial-mediated pathway and
involvement of NF-kB. BMC Compl Alternative
Med. 14:1-13.
6. Moghadamtousi SZ, E Rouhollahi, H Karimian, M
Fadaeinasab, M Firoozinia, MA Abdulla, HA Kadir.
2015. The Chemopotential Effect of Annona
muricata Leaf against Azoxymethane-Induced
Colonic Aberrant Crypt Foci in Rats and the
Apoptotic Effect of Acetogenin Annomuricin E in
HT-29 Cells: A Bioassay-Guided Approach. J Pone.
DOI :10.1371:1-28.

7. Artini NPR, S Wahjuni, WD Sutiningtyas. 2012.
Soursop Extract (Annona muricata L.) as
Antioxidant for Reduce Uric Acid Winstar Rats. J
Chem. 6:127-37.
8. Gajalaksmi S, S Vijayalakshmi, DV Rajeswari. 2012.
Phytochemical and Pharmacological Properties
of Annona muricata: Review. Int’l J Pharm Pham
Sci. 4:1-6.
9. Hasan AEZ, D Mangunwidjaja, TC Sunarti, O
Suparno, A Setiyono. 2014. Investigating the
antioxidant and anticytotoxic activities of
propolis collected from five regions of Indonesia
and their abilities to induce apoptosis. Emir J
Food Agric. 26:390-8.
10. Hasan AEZ, L Ambarsari, I M Artika, H Julistiono,
D. Tarunasari. 2013. Induction resistance of
Candida sp. Y 390 to ethanol stress by kopyor
coconut and virgin coconut oil. Emir J Food
Agric. 25:790-7.
11. Jang MJ, Sheu SR, Wang CC, Yeh YL, Sung KH.
2009. Optimization Analysis of the Experimental
Parameters on the Extraction Process of Propolis.
Proceedings of the International Multi
Conference of Engineers and Computer
Scientists. II, IMECS. Hongkong. 1295-9.
12. Zhang HF, Yang XH, Wang Y. 2011. Microwave
assisted extraction of secondary metabolites
from plants: Current status and future directions.
Trends Food Sci Technol. 22: 672-88.
13. Harborne, JB. 1987. Phytochemical Methods.
Chapman and Hall, London, UK., Pages: 1-286.
14. Melati M, Aziz SA, Ghulamahdi M. 2011. Ordinary
Java Chili studies (Piper retro fractum Vahl.) and
Chili Java Shrubs of Three Production Center with
Light Intensity and Diversity Fertilization.
http://repository.ipb.ac.id/bitstream/handle/
123456789/41764/ Maya%20MelatiStudi_Cabe%20Jawa_HB.pdf?sequence=3

[06
January 2014].
15. Hasan AEZ, Suryani, S. Estuningsih, Fitriannur.
2015. Enterobacter sakazakii be Inhibited by
Trigona spp Propolis from Pandeglang Indonesia.
Int’l J Pharm Bio Sci. 6:420-8.
16. Bezerra DP, FO Castro, APNN Alves, C Pessoa,
MO Moraes, ER. Silveira, et al. 2006. In
vivo growth-inhibition of Sarcoma 180 by

piplartine and piperine, two alkaloid amides
from Piper. Braz J Med Biol Res. 39:801-6.
17. Abrahim NN, MS Kanthimathi and A Abdul-Aziz.
2012. Piper betle shows antioxidant activities,
inhibits MCF-7 cell proliferation and increases
activities of catalase and superoxide dismutase.
BMC Complement Alternat Med. 12:1-11.
18. Lin L, PL Hwang. 1991. Antiproliferative Effects of
Oxygenated Sterols: Positive Corellation with
Binding Affinities for the Estrogen-Binding Sites.
Biochem Biophys Acta. 1082:
https://doi.org/10.1016/0005-2760(91)90192-K.
19. Fidianingsih I and ES Handayani. 2014. Annona
muricata aqueous extract suppresses T47D
breast cancer cell proliferation. Univ Med. 3:1926.

20. Champy P, Alice Melot, V Guérineau, C Gleye,
D Fall, Gunter U, et al. 2005. Quantification of
acetogenins in Annona muricata linked to atypical
parkinsonism in guadeloupe. Movement
Disorders. 20:1629–33.
21. Champy P, Höglinger GU, Féger J, Gleye C,
Hocquemiller R, Laurens A, et al. 2004.
Annonacin, a lipophilic inhibitor of mitochondrial
complex I, induces nigral and striatal
neurodegeneration in rats: possible relevance for
atypical parkinsonism in Guadeloupe. J
Neurochem. 88:63-9.
22. Pomper KW. 2009. Acetogenin Update.
www.pawpaw. Kysu.edu/PDF /AcetoUpdate3.pdf
(January 2013).
23. Zeng L, Wu FE, Oberlies NH, Mc Laughlin JL,
Sastrodihardjo S. 1996. Five new
monotetrahydrofuran ring acetogenins from the
leaf of Annona muricata. J Nat Prod. 59:1035-42.
24. Consolacion RY, S Geneveve, OB Torres, D MingJaw,
S Chien-Chang.
2012.
Acetogenins
from

Annona
muricata.
Phycog
J.
2:32-7.

25. Rodríguez FJM, DM Pinedo, M Nodarse. 2010.
Assessment of scientific evidence recommending
Annona muricata L. (soursop tree) for cancer
prevention or treatment. Rev Cubana Plant Med.
15:169-81.

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Detail Karya Ilmiah Dosen

Akhmad Endang Zainal Hasan, Nurliani Bermawie, Heddy Julistiono, Eny Ida Riyanti, Hasim Hasim, Usep Suhendar

Judul	:	PHYTOCHEMICAL SCREENING AND ANTI-BREASTCANCER ACTIVITIES OF ANNONA MURICATA (L.) LEAF EXTRACTS
Abstrak	:	The objective of the research is to study antiproliferation activities (anti breastcancer) of leaf extracts soursop (Annona muricata L.) from five locations growth in West Java, Indonesia against breast-cancer cell line Michigan Cancer Foundation-7 (MCF-7). Each of the soursop leaf from five locations, was extracted with 70% ethanol using a microwave as a tool for extraction. In vitro test against breast cancer cells growth was conducted using the MCF-7 cell line by means of the MTT-assay.. Based on the analysis of phytochemicals, all soursop leaf extracts contain alkaloids, triterpenoids, tannins, saponins and flavonoids, but did not contain any steroids compound. Soursop leaf extracts with the highest anticancer activity were derived from Sukabumi 1 with an IC50 value of 12.5 µg/ml. The results obtained from this study indicate the ability of soursop leaf extracts to inhibite the growth of breast cancer cells (MCF-7). Such extracts could be used in the treatment of cancer patients.
Tahun	:	2020	Media Publikasi	:	Jurnal Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	24 / 2 / 2020
ISSN/ISBN	:	1827-8620
PTN/S	:	Universitas Pakuan	Program Studi	:	FARMASI
Bibliography	:	1. [WHO] World Health Organization. The Global Burden of Disease: 2004 Update. 2008. Geneva: World Health Organization. htt://www.who.int/healthinfo/global_burden_ disease/GBD_report_2004update_part1.pdf [29 October 2010]. 2. [Ministry of Health] Kementerian Kesehatan Republik Indonesia. 2013. http://www.depkes.go.id/index.php/berita/pressrelease/2233-seminar-sehari -dalam-rangkamemperingati-hari-kanker-sedunia-2013.html [14 June 2013]. 3. Solomon-Wisdom GO, Ugoh SC, Muhammad B. 2014. Phytochemical Screening and Antimicrobial activities of Annona muricata (L) leaf extract. Am J Biol Chem Pharm Sci.2:1-7. 4. Paarakh PM, JPN Chansouria, RL Khosa. 2009. Wound Healing Activity of Annona muricata Extract. J Pharm Res. 2: 404-6. 5. Moghadamtousi SZ, HA Kadir, M Paydar, E Rouhollai, H Karimian. 2014. Annona muricata leaf induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-kB. BMC Compl Alternative Med. 14:1-13. 6. Moghadamtousi SZ, E Rouhollahi, H Karimian, M Fadaeinasab, M Firoozinia, MA Abdulla, HA Kadir. 2015. The Chemopotential Effect of Annona muricata Leaf against Azoxymethane-Induced Colonic Aberrant Crypt Foci in Rats and the Apoptotic Effect of Acetogenin Annomuricin E in HT-29 Cells: A Bioassay-Guided Approach. J Pone. DOI :10.1371:1-28. 7. Artini NPR, S Wahjuni, WD Sutiningtyas. 2012. Soursop Extract (Annona muricata L.) as Antioxidant for Reduce Uric Acid Winstar Rats. J Chem. 6:127-37. 8. Gajalaksmi S, S Vijayalakshmi, DV Rajeswari. 2012. Phytochemical and Pharmacological Properties of Annona muricata: Review. Int’l J Pharm Pham Sci. 4:1-6. 9. Hasan AEZ, D Mangunwidjaja, TC Sunarti, O Suparno, A Setiyono. 2014. Investigating the antioxidant and anticytotoxic activities of propolis collected from five regions of Indonesia and their abilities to induce apoptosis. Emir J Food Agric. 26:390-8. 10. Hasan AEZ, L Ambarsari, I M Artika, H Julistiono, D. Tarunasari. 2013. Induction resistance of Candida sp. Y 390 to ethanol stress by kopyor coconut and virgin coconut oil. Emir J Food Agric. 25:790-7. 11. Jang MJ, Sheu SR, Wang CC, Yeh YL, Sung KH. 2009. Optimization Analysis of the Experimental Parameters on the Extraction Process of Propolis. Proceedings of the International Multi Conference of Engineers and Computer Scientists. II, IMECS. Hongkong. 1295-9. 12. Zhang HF, Yang XH, Wang Y. 2011. Microwave assisted extraction of secondary metabolites from plants: Current status and future directions. Trends Food Sci Technol. 22: 672-88. 13. Harborne, JB. 1987. Phytochemical Methods. Chapman and Hall, London, UK., Pages: 1-286. 14. Melati M, Aziz SA, Ghulamahdi M. 2011. Ordinary Java Chili studies (Piper retro fractum Vahl.) and Chili Java Shrubs of Three Production Center with Light Intensity and Diversity Fertilization. http://repository.ipb.ac.id/bitstream/handle/ 123456789/41764/ Maya%20MelatiStudi_Cabe%20Jawa_HB.pdf?sequence=3 [06 January 2014]. 15. Hasan AEZ, Suryani, S. Estuningsih, Fitriannur. 2015. Enterobacter sakazakii be Inhibited by Trigona spp Propolis from Pandeglang Indonesia. Int’l J Pharm Bio Sci. 6:420-8. 16. Bezerra DP, FO Castro, APNN Alves, C Pessoa, MO Moraes, ER. Silveira, et al. 2006. In vivo growth-inhibition of Sarcoma 180 by piplartine and piperine, two alkaloid amides from Piper. Braz J Med Biol Res. 39:801-6. 17. Abrahim NN, MS Kanthimathi and A Abdul-Aziz. 2012. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase. BMC Complement Alternat Med. 12:1-11. 18. Lin L, PL Hwang. 1991. Antiproliferative Effects of Oxygenated Sterols: Positive Corellation with Binding Affinities for the Estrogen-Binding Sites. Biochem Biophys Acta. 1082: https://doi.org/10.1016/0005-2760(91)90192-K. 19. Fidianingsih I and ES Handayani. 2014. Annona muricata aqueous extract suppresses T47D breast cancer cell proliferation. Univ Med. 3:1926. 20. Champy P, Alice Melot, V Guérineau, C Gleye, D Fall, Gunter U, et al. 2005. Quantification of acetogenins in Annona muricata linked to atypical parkinsonism in guadeloupe. Movement Disorders. 20:1629–33. 21. Champy P, Höglinger GU, Féger J, Gleye C, Hocquemiller R, Laurens A, et al. 2004. Annonacin, a lipophilic inhibitor of mitochondrial complex I, induces nigral and striatal neurodegeneration in rats: possible relevance for atypical parkinsonism in Guadeloupe. J Neurochem. 88:63-9. 22. Pomper KW. 2009. Acetogenin Update. www.pawpaw. Kysu.edu/PDF /AcetoUpdate3.pdf (January 2013). 23. Zeng L, Wu FE, Oberlies NH, Mc Laughlin JL, Sastrodihardjo S. 1996. Five new monotetrahydrofuran ring acetogenins from the leaf of Annona muricata. J Nat Prod. 59:1035-42. 24. Consolacion RY, S Geneveve, OB Torres, D MingJaw, S Chien-Chang. 2012. Acetogenins from Annona muricata. Phycog J. 2:32-7. 25. Rodríguez FJM, DM Pinedo, M Nodarse. 2010. Assessment of scientific evidence recommending Annona muricata L. (soursop tree) for cancer prevention or treatment. Rev Cubana Plant Med. 15:169-81.
URL	: