Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Tri Saptari Haryani 1 ,Bina Lohitasari2 And Triastinurmiatiningsih3

Judul : Toxicity and Compound Identification of PadinaaustralisExtract

Abstrak :
Abstract. People awareness of the importance of health has increased significantly in

the last decade, it forces people to find alternative treatments which are cheaper and safer when compared with the use of synthetic drugs. Research on antibacterial activity of Padina australis against Escherichia coli have been conducted, but it does not identified yet which active compounds in the Padina australis extract that has potential as an antibacterial. Identification phytol compounds from the extracts of Padina australis and toxicity tests have been conducted using BSLT and GCMS methods. The results of this research identify that Padina australis extract with ethanol has antibacterial activity value of LC50 177.83 ppm. Phytochemical test results show that active ingredient of Padina australis extract is terpenoid compounds, and that triterpenoids has potential as an antibacterial. GC-MS test result shows that active compound of Padina australis extract consists of phytol compound which has 90-99% similarity with steroids, phenols, fatty acids, carboxylic acids, hydrocarbons, and proteins. Compound identification test of active Padina australis extracts through GC-MS methods show that phytol compounds are useful as antibacterial and the toxicity test results show that phytol compounds are not cytotoxic.

        Keywords: antibacterial, extract Padina australis, LC50

Tahun : 2018 Media Publikasi : Seminar Internasional

Kategori : Jurnal No/Vol/Tahun : - / - / 2018

ISSN/ISBN : -

PTN/S : Universitas Pakuan Program Studi : BIOLOGI

Bibliography :
[1] Bhat, S. V. B. A. Nagasampagi and S. Meenakshi. 2009. Natural Product Chemistry and Application. Narosa Publishing House: New Delhi, India.

[2] Bigham, A. K., Munro, A. T, Rizzacasa, M. A., Roy, M., and Browne R. 2003. Divinatorins A-c, New

Neoclerodane Diterpenoid from controlled sage Silvia divinorum. Melbourn University, Victoria, 3010, Australia; hal. 32-33.

[3] Fitriany, P. 2012. Kandungan Fenol, Senyawa Fitokimia, Aktivitas Antioksidan Rumput Laut Padina australis. Bogor. Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Alamat (http://repository.ipb.ac.id/handle/123456789/56754)

[4] Fitrya, I. Soediro, dan Kusmardiyani S. 2010. Pemeriksaan Karakter Simplisia Alga Padina australis Hauck (Dyctotaceae). Bandung: Sekolah Pasca Sarjana, Institut teknologi Bandung

[5] Grayson, D. H. 2000. Monoterpenoids. University Chemical Laboratory. Trinity Collage Dublin 2, Ireland pp: 32-33

[6] Goldberg, L. 1994. Functional Food, Designer Food, Pharma Food, Neutraceuticals. Chapman and Hall: New York.

[7] Harborne, J. B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Institut   Teknologi Bandung, Bandung. Hal. 234-236.

[8] Haryani, T.S. dan Triastinurmiatiningsih. 2014. Pengembangan Formula Tablet Ekstrak Padina australis Sebagai Antibakteri Escherichia coli Penyebab Penyakit Diare. Laporan Hibah Penelitian Kemenristek Dikti, Jakarta.

[9] Juniarti, D. Osmeli dan Yuhernita. 2009. Kandungan Senyawa Kimia, Uji Toksisitas,   (Brine Shrimp Lethality Test) dan Antioksidan (1,1-diphenyl- 2 pikrilhydrazyl) dari Ekstrak Daun Saga (Abrus precatorius l.). Makara Sains13 (1): 50-54.

[10] Kim, K.-N., Heo, S.-J., Yoon, W.-J., Kang, S.-M., Ahn, G.Yi, T.-H., & Jeon, Y.-J. 2010. Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-κB and MAPKs in lipopolysaccharide-induced RAW 264.7 macrophages. European J. Pharmacology, 649: 369-375.

[11] Krishnaraju, A. V, T. V. N. Rao, D. Sundararaju, M. Vanisree, H. S. Tsay and G.V. Subbaraju. 2005. Assessment   of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering, 3 (2): 125- 134.

[12] Limantara, L., & Heriyanto. 2010. Studi Komposisi Pigmen dan Kandungan Fukosantin Rumput Laut Coklat dari Perairan Madura dengan Kromatografi Cair Kinerja Tinggi. Ilmu Kelautan, 15(1): 23-32.

[13] Lim. S. Y., Bauermeister A., Kjonaas, R. A. and Gosh S. K. 2006. Phytol Based Novel Adjuvants in vaccine Formulation: 2 Assement of Efficacy in the Induction of Protective Immune Responses to Lethal Bacterial Infections in Mice. Departement of Life Science, Indiana State University, Terre Haute, IN 47809 USA (hal 32-33)

[14] McLaughlin JL & LL Rogers. The use of biological assay to evaluate botanicals. Drug Information Journal, 1998; 32: P. 513-24

[15] Meyer, B. N., N. R. Ferrigni, J. E. Putman, L. B. Jacbsen, D. E. Nicols and J. L.McLaughlin. 1982. Brine Shrimp: A Comvenient general Bioassay for Active Plant Constituents. Planta Medica 45: 31-34.

[16]   Nurhayati T.D, Aryani, dan Nurjanah. 2009.

         Kajian Awal Potensi Ekstrak. Jurnal Kelautan Nasional 2: hal. 43-51

[17]   Pavia, Donald L., Gary M. Lampman, George S. Kritz, Randall G. Engel (2006). Introduction to Organic Laboratory Techniques (4th Ed.). Thomson Brooks/Cole. P. 797–817.

[18]    Rasyid, A. 2005. Beberapa Catatan Tentang Alginat. Oseana Volume XXX No. 1: 9-14

[19]   Restasari, A., D. Kusrini, dan E. Fachriyah. 2009. Isolasi dan identifikasi Fraksi   Teraktif dari Ekstrak Kloroform Daun Ketapang (Terminalia catappa Linn). FMIPA Kimia UNDIP. Semarang

[20]   Santoso, J., S. Anwariyah, R. O. Rumiantin, A. P. Putri, N. Ukhty and Y. Yoshie- Stark. 2012. Phenol Content, Antioxidant Activity and Fibers profile of Four Tropical Seagrasses from Indonesia. Journal of Coastal Development, 15 (2): 189-196.

[21]   Suganda, A.G., Swandari S., Dewi. R., Sukmawan R.H., Maeka L, Ratna A.L. 2007. Telaah Kandungan Kimia Padina australis Hauck (Dyctotacae). Detail Penelitian Obat Bahan Alam. Sekolah Farmasi ITB . (http://bahan-alam-fa.itb.co.id)

[22]   Suryanto E, Wehantou F, Raharjo S. 2008. Aktivitas penstabilan senyawa oksigen reaktif dari beberapa herbal. Jurnal    Obat Bahan Alam 7 (1): 62-68

[23]   Susanto, A. 2008. Kandungan Sumber Daya Hayati Alga Coklat. Jakarta: Gramedia

[24]   Tamat, S. R., T. Wikanta dan L. S. Maulina. 2007. Aktivitas Antioksidan dan Toksisitas Senyawa Bioaktif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal. Jurnal Ilmu Kefarmasian Indonesia, 5 (1): 31-36.

[25]   Zakaria, N. A., D. Ibrahim, S. F. Sulaiman and N. A. Supardy. 2011. Assassment of Antioxidant Activity, Total Phenolic Content and Invitro Toxicity of Malaysian Red Seaweed, Acanthophora spicifera.

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Detail Karya Ilmiah Dosen

Tri Saptari Haryani 1 ,Bina Lohitasari2 And Triastinurmiatiningsih3

Judul	:	Toxicity and Compound Identification of PadinaaustralisExtract
Abstrak	:	Abstract. People awareness of the importance of health has increased significantly in the last decade, it forces people to find alternative treatments which are cheaper and safer when compared with the use of synthetic drugs. Research on antibacterial activity of Padina australis against Escherichia coli have been conducted, but it does not identified yet which active compounds in the Padina australis extract that has potential as an antibacterial. Identification phytol compounds from the extracts of Padina australis and toxicity tests have been conducted using BSLT and GCMS methods. The results of this research identify that Padina australis extract with ethanol has antibacterial activity value of LC50 177.83 ppm. Phytochemical test results show that active ingredient of Padina australis extract is terpenoid compounds, and that triterpenoids has potential as an antibacterial. GC-MS test result shows that active compound of Padina australis extract consists of phytol compound which has 90-99% similarity with steroids, phenols, fatty acids, carboxylic acids, hydrocarbons, and proteins. Compound identification test of active Padina australis extracts through GC-MS methods show that phytol compounds are useful as antibacterial and the toxicity test results show that phytol compounds are not cytotoxic. Keywords: antibacterial, extract Padina australis, LC50
Tahun	:	2018	Media Publikasi	:	Seminar Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	- / - / 2018
ISSN/ISBN	:	-
PTN/S	:	Universitas Pakuan	Program Studi	:	BIOLOGI
Bibliography	:	[1] Bhat, S. V. B. A. Nagasampagi and S. Meenakshi. 2009. Natural Product Chemistry and Application. Narosa Publishing House: New Delhi, India. [2] Bigham, A. K., Munro, A. T, Rizzacasa, M. A., Roy, M., and Browne R. 2003. Divinatorins A-c, New Neoclerodane Diterpenoid from controlled sage Silvia divinorum. Melbourn University, Victoria, 3010, Australia; hal. 32-33. [3] Fitriany, P. 2012. Kandungan Fenol, Senyawa Fitokimia, Aktivitas Antioksidan Rumput Laut Padina australis. Bogor. Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Alamat (http://repository.ipb.ac.id/handle/123456789/56754) [4] Fitrya, I. Soediro, dan Kusmardiyani S. 2010. Pemeriksaan Karakter Simplisia Alga Padina australis Hauck (Dyctotaceae). Bandung: Sekolah Pasca Sarjana, Institut teknologi Bandung [5] Grayson, D. H. 2000. Monoterpenoids. University Chemical Laboratory. Trinity Collage Dublin 2, Ireland pp: 32-33 [6] Goldberg, L. 1994. Functional Food, Designer Food, Pharma Food, Neutraceuticals. Chapman and Hall: New York. [7] Harborne, J. B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Institut Teknologi Bandung, Bandung. Hal. 234-236. [8] Haryani, T.S. dan Triastinurmiatiningsih. 2014. Pengembangan Formula Tablet Ekstrak Padina australis Sebagai Antibakteri Escherichia coli Penyebab Penyakit Diare. Laporan Hibah Penelitian Kemenristek Dikti, Jakarta. [9] Juniarti, D. Osmeli dan Yuhernita. 2009. Kandungan Senyawa Kimia, Uji Toksisitas, (Brine Shrimp Lethality Test) dan Antioksidan (1,1-diphenyl- 2 pikrilhydrazyl) dari Ekstrak Daun Saga (Abrus precatorius l.). Makara Sains13 (1): 50-54. [10] Kim, K.-N., Heo, S.-J., Yoon, W.-J., Kang, S.-M., Ahn, G.Yi, T.-H., & Jeon, Y.-J. 2010. Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-κB and MAPKs in lipopolysaccharide-induced RAW 264.7 macrophages. European J. Pharmacology, 649: 369-375. [11] Krishnaraju, A. V, T. V. N. Rao, D. Sundararaju, M. Vanisree, H. S. Tsay and G.V. Subbaraju. 2005. Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering, 3 (2): 125- 134. [12] Limantara, L., & Heriyanto. 2010. Studi Komposisi Pigmen dan Kandungan Fukosantin Rumput Laut Coklat dari Perairan Madura dengan Kromatografi Cair Kinerja Tinggi. Ilmu Kelautan, 15(1): 23-32. [13] Lim. S. Y., Bauermeister A., Kjonaas, R. A. and Gosh S. K. 2006. Phytol Based Novel Adjuvants in vaccine Formulation: 2 Assement of Efficacy in the Induction of Protective Immune Responses to Lethal Bacterial Infections in Mice. Departement of Life Science, Indiana State University, Terre Haute, IN 47809 USA (hal 32-33) [14] McLaughlin JL & LL Rogers. The use of biological assay to evaluate botanicals. Drug Information Journal, 1998; 32: P. 513-24 [15] Meyer, B. N., N. R. Ferrigni, J. E. Putman, L. B. Jacbsen, D. E. Nicols and J. L.McLaughlin. 1982. Brine Shrimp: A Comvenient general Bioassay for Active Plant Constituents. Planta Medica 45: 31-34. [16] Nurhayati T.D, Aryani, dan Nurjanah. 2009. Kajian Awal Potensi Ekstrak. Jurnal Kelautan Nasional 2: hal. 43-51 [17] Pavia, Donald L., Gary M. Lampman, George S. Kritz, Randall G. Engel (2006). Introduction to Organic Laboratory Techniques (4th Ed.). Thomson Brooks/Cole. P. 797–817. [18] Rasyid, A. 2005. Beberapa Catatan Tentang Alginat. Oseana Volume XXX No. 1: 9-14 [19] Restasari, A., D. Kusrini, dan E. Fachriyah. 2009. Isolasi dan identifikasi Fraksi Teraktif dari Ekstrak Kloroform Daun Ketapang (Terminalia catappa Linn). FMIPA Kimia UNDIP. Semarang [20] Santoso, J., S. Anwariyah, R. O. Rumiantin, A. P. Putri, N. Ukhty and Y. Yoshie- Stark. 2012. Phenol Content, Antioxidant Activity and Fibers profile of Four Tropical Seagrasses from Indonesia. Journal of Coastal Development, 15 (2): 189-196. [21] Suganda, A.G., Swandari S., Dewi. R., Sukmawan R.H., Maeka L, Ratna A.L. 2007. Telaah Kandungan Kimia Padina australis Hauck (Dyctotacae). Detail Penelitian Obat Bahan Alam. Sekolah Farmasi ITB . (http://bahan-alam-fa.itb.co.id) [22] Suryanto E, Wehantou F, Raharjo S. 2008. Aktivitas penstabilan senyawa oksigen reaktif dari beberapa herbal. Jurnal Obat Bahan Alam 7 (1): 62-68 [23] Susanto, A. 2008. Kandungan Sumber Daya Hayati Alga Coklat. Jakarta: Gramedia [24] Tamat, S. R., T. Wikanta dan L. S. Maulina. 2007. Aktivitas Antioksidan dan Toksisitas Senyawa Bioaktif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal. Jurnal Ilmu Kefarmasian Indonesia, 5 (1): 31-36. [25] Zakaria, N. A., D. Ibrahim, S. F. Sulaiman and N. A. Supardy. 2011. Assassment of Antioxidant Activity, Total Phenolic Content and Invitro Toxicity of Malaysian Red Seaweed, Acanthophora spicifera.
URL	:	-