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Detail Karya Ilmiah Dosen

Tri Saptari Haryani, Triastinurmiatiningsih, Bina Lohita Sari

Judul : Antioxidant Test Extract Of Padina australis And Acute Toxicity Effect on Mice (Mus muculus)

Abstrak :
Antioxidant Test Extract Of Padina australis And Acute Toxicity Effect

on Mice (Mus muculus)

Abstract

Seaweed has polyphenol aggregates that provide antioxidant, one of which is Padina australis possesses antioxidant activity. These tests demonstrated to test the elevations of polyphenolic compounds that can measure out as antioxidants and analyze the component volume of extract of P. australis who have crucial toxic impacts on mice as experiment animal. Stages of evaluation include P.australis extraction by maceration method using ethanol 96%, therefore process assessment of overall polyphenol extracts of P. australis with Folin Ciocalteu and absorbance method involving UV-Vis spectrophotometer at an observation of 750 nm, examine the antioxidant activity using DPPH process ( 1.1diphenyl-2-picrihydration/DPPH) at an observation of 515 nm calculated on the base of the narrow regression equation and the method in IC50 (ppm). Measurement of vital toxicity of P. australis extract using Swiss Webster mice as an experiment animal for that activity, evaluation processes, defecation tests, and salivation tests. From result of study of comprehensive polyphenol content of extract result 69.16mg SAG/gram with absorbance rate analogous to 0.115. From evaluating the antioxidant activity observed by IC50 is 3265.64 ppm. From the results of crucial toxicity evaluation in mice as an experiment animal, at the highest dosage of 2000 mg / body weight, it can be stated that P.australis extract is not toxic.

Keywords: Extract of Padina australis, antioxidant, acute toxicity effect

Tahun : 2017 Media Publikasi : Seminar Internasional

Kategori : Poster No/Vol/Tahun : - / - / 2017

ISSN/ISBN : -

PTN/S : Universitas Pakuan Program Studi : BIOLOGI

Bibliography :
Dotulong, V., Widjanarko, SB, Yunianta and Mamahit, L.P. 2014. Antioxidant Activity of Three Marine Algae Methanol Extract Collected from North Sulawesi Waters, Indonesia. International Journal of Science and Engineering Investigations. 2(23):26-30.

Firdaus, M. (2011). Aktivitas Antioksidan Ekstrak Rumput Laut Coklat (Sargassum echinocarpum) sebagai Pencegah Disfungsi Sel Endotelium Aorta Tikus Diabetes Melitus. Disertasi. Sekolah Pascasarjana IPB. Bogor

Haryani, TS. Bina Lohitasari, dan Triastinurmiatiningsih, 2014.

Efektivitas Ekstrak Padina australis Sebagai Antibakteri Escherichia coli Penyebab Diare. Vol 4:2, Desember 2014. Jurnal Fitofarmaka, Universitas Pakuan, Bogor.

Husni, A., Putra, D.R., Bambang, I.Y. 2014. Aktivitas Antioksidan Padina sp Pada Berbagai Suhu dan Lama Pengeringan. UGM. Jogyakarta. JPG. Perikanan. Vol. 9:2 ; 165-177

Triastinurmiatiningsih dan T.S. Haryani. 2008. Potensi Rumput Laut Di Pantai Bayah, Kabupaten Lebak, Banten

Sebagai Anti Bakteri Escherichia coli. Jurnal Matematika, Sains dan Teknologi, Volume 9, no 1, hal 37-43.

Zubia, M., Robledo, D., & Freile-Pelegrin Y. 2007. Antioxidant activities in marine macroalgae from the coasts of quintana Roo and Yucatan, Mexico. Journal of Applied Phycology. 19: 449–458.

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Detail Karya Ilmiah Dosen

Tri Saptari Haryani, Triastinurmiatiningsih, Bina Lohita Sari

Judul	:	Antioxidant Test Extract Of Padina australis And Acute Toxicity Effect on Mice (Mus muculus)
Abstrak	:	Antioxidant Test Extract Of *Padina australis* And Acute Toxicity Effect on Mice *(Mus muculus)* Abstract Seaweed has polyphenol aggregates that provide antioxidant, one of which is Padina australis possesses antioxidant activity. These tests demonstrated to test the elevations of polyphenolic compounds that can measure out as antioxidants and analyze the component volume of extract of P. australis who have crucial toxic impacts on mice as experiment animal. Stages of evaluation include P.australis extraction by maceration method using ethanol 96%, therefore process assessment of overall polyphenol extracts of P. australis with Folin Ciocalteu and absorbance method involving UV-Vis spectrophotometer at an observation of 750 nm, examine the antioxidant activity using DPPH process ( 1.1diphenyl-2-picrihydration/DPPH) at an observation of 515 nm calculated on the base of the narrow regression equation and the method in IC50 (ppm). Measurement of vital toxicity of P. australis extract using Swiss Webster mice as an experiment animal for that activity, evaluation processes, defecation tests, and salivation tests. From result of study of comprehensive polyphenol content of extract result 69.16mg SAG/gram with absorbance rate analogous to 0.115. From evaluating the antioxidant activity observed by IC50 is 3265.64 ppm. From the results of crucial toxicity evaluation in mice as an experiment animal, at the highest dosage of 2000 mg / body weight, it can be stated that P.australis extract is not toxic. Keywords: Extract of Padina australis, antioxidant, acute toxicity effect
Tahun	:	2017	Media Publikasi	:	Seminar Internasional
Kategori	:	Poster	No/Vol/Tahun	:	- / - / 2017
ISSN/ISBN	:	-
PTN/S	:	Universitas Pakuan	Program Studi	:	BIOLOGI
Bibliography	:	Dotulong, V., Widjanarko, SB, Yunianta and Mamahit, L.P. 2014. Antioxidant Activity of Three Marine Algae Methanol Extract Collected from North Sulawesi Waters, Indonesia. International Journal of Science and Engineering Investigations. 2(23):26-30. Firdaus, M. (2011). Aktivitas Antioksidan Ekstrak Rumput Laut Coklat (Sargassum echinocarpum) sebagai Pencegah Disfungsi Sel Endotelium Aorta Tikus Diabetes Melitus. Disertasi. Sekolah Pascasarjana IPB. Bogor Haryani, TS. Bina Lohitasari, dan Triastinurmiatiningsih, 2014. Efektivitas Ekstrak Padina australis Sebagai Antibakteri Escherichia coli Penyebab Diare. Vol 4:2, Desember 2014. Jurnal Fitofarmaka, Universitas Pakuan, Bogor. Husni, A., Putra, D.R., Bambang, I.Y. 2014. Aktivitas Antioksidan Padina sp Pada Berbagai Suhu dan Lama Pengeringan. UGM. Jogyakarta. JPG. Perikanan. Vol. 9:2 ; 165-177 Triastinurmiatiningsih dan T.S. Haryani. 2008. Potensi Rumput Laut Di Pantai Bayah, Kabupaten Lebak, Banten Sebagai Anti Bakteri Escherichia coli. Jurnal Matematika, Sains dan Teknologi, Volume 9, no 1, hal 37-43. Zubia, M., Robledo, D., & Freile-Pelegrin Y. 2007. Antioxidant activities in marine macroalgae from the coasts of quintana Roo and Yucatan, Mexico. Journal of Applied Phycology. 19: 449–458.
URL	:	-