Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Siti Warnasih, Diana Widiastuti, Uswatun Hasanah, Laksmi Ambarsari, Purwantiningsih Sugita

Judul : Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts
Abstrak :

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants.

Tahun : 2019 Media Publikasi : Jurnal Internasional
Kategori : Jurnal No/Vol/Tahun : 2S7 / 8 / 2019
ISSN/ISBN : 2277-3878
PTN/S : Universitas Pakuan Program Studi : KIMIA
Bibliography :

1. F. Pourmorad, S. J Hosseinimehr, N. Shahabimajd, “Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants.” African Journal of Biotechnology, vol. 5, no. 11, pp. 1142–1145, 2006.
2. M. Al-Farsi, C. Alasalvar, M. Al-Abid, K. Al-Shoaily, M. Al-Amry and F. Al-Rawahy, “Compositional and functional characteristics of dates, syrups, and their by-products.” Food Chemistry, vol. 104, no. 3, pp. 943–947, 2007.
3. N. Chaira, A. Ferchichi, A. Mrabet and M. Sghairoun, “Chemical composition of the flesh and the pit of date palm fruit and radical scavenging activity of their extracts.” Pakistan Journal of Biological Sciences,vol. 10, no. 13, pp. 2202–2207, 2007.
4. B. R. Yasin, H. A. N. El-Fawal and S. A. Mousa, “Date (Phoenix dactylifera) polyphenolics and other bioactive compounds: A traditional islamic remedy’s potential in prevention of celldamage, cancer therapeutics and beyond.” International Journal of Molecular Sciences,vol. 16, no. 12, pp. 30075–30090, 2015.
5. M. A. Al-Farsi and C. Y. Lee, “Optimization of phenolics and dietary fibre extraction from date seeds.” Food Chemistry, vol. 108, no. 3, pp. 977–985, 2008.
6. E. Bouhlali, T. Dine, C. Alem, J. Ennassir, M. Benlyas, A. N. Mbark and Y. F. Zegzouti, “Phytochemical compositions and antioxidant capacity of three date (Phoenix dactylifera L.) seeds varieties grown in the South East Morocco.” Journal of the Saudi Society of Agricultural Sciences, vol. 16, no. 4, pp. 350–357, 2015. 7. W. Horwitz. Official methods of analysis. Washington: Association of Official Analytical Chemists, 1975.
8. J. B. Harborne, Phytochemical Methods. London: Chapman and Hall Ltd, 1987.
9. Departemen Kesehatan Republik Indonesia, Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan, 2000.
10. A. M. Adeosun, S. O. Oni, O. M. Ighodaro, O. H. Durosinlorun and O. M. Oyedele, “Phytochemical, minerals and free radical scavenging profiles of Phoenix dactilyfera L. seed extract.” Journal of Taibah University Medical Sciences, vol. 11, no. 1, pp. 1–6, 2016.
11. P. Molyneux, “The Use of the stable free radical Diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity.” Songklanakarin Journal of Science and Technology, vol. 26, no. 2, pp. 211–219, 2004.
12. M. R. S. Ardekani, M. Khanavi, M. Hajimahmoodi, M. Jahangiri, A. Hadjiakhoondi, “Comparison of antioxidant activity and total phenol contents of some date seed varieties from Iran.” Iranian Journal of Pharmaceutical Research, vol. 9, no. 2, pp. 141–146, 2010.
13. R. Messaoudi, S. Abbeddou, A. Mansouri, A. C. Calokerinos, P. Kefalas, “Phenolic profile and antioxidant activity of date-pits of seven algerian date palm fruit varieties.” International Journal of Food Properties, vol. 16, no. 5, 1037–1047, 2013.

URL :

 

Document

 
back