Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Barahima Abbas1, Muhammad Dailami2, Florentina Heningtyas Listyorini1, Munarti3

Judul : Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers
Abstrak :

Orchidaceae has known as an attractive flower and immense species. We have found a large
species of Orchidaceae grow naturally in Papua’s jungle, Indonesia territorial. This study
aims to reveal genetic variation and genetic relationships among endemic orchids in Papua
based on RAPD markers. The study included 26 accessions of Papua’s endemic orchids used
for genomic DNA extraction. Genomic DNAs were extracted by using DNA extraction kit
from Qiagen and genomic DNA amplification by using 10 decamer RAPD primers. DNA
fragments that were amplified by Polymerase Change Reaction (PCR) were visualized and
documented by using UV illumination apparatus. Genetically, endemic Orchids in Papua
were described high variation. Fragments amplification by using ten RAPD primers and
performed in the PCR tools resulted in 54 numbers of polymorphic fragments and no monomorphic
band. The number of polymorphic bands per primer ranged from 4 to 7 with
averaged 5.4 bands per assay unit. The genetic dissimilarities (GDs) among examined orchids
ranged from 0.10 to 0.94 based on Nei’s unbiased coefficients. Dendrogram construction
showed that Papua’s endemic orchid (PEO) samples different from another and separated
to form group by their own at the 0.40 coefficient value and at the 0.6 coefficient value
indicate that PEO sample is divided into nine groups i.e. samples at the genera level were
separated into their own groups.

Tahun : 2017 Media Publikasi : Jurnal Internasional
Kategori : Jurnal No/Vol/Tahun : 11 / 9 / 2017
ISSN/ISBN : ISSN Online 2150-4105
PTN/S : University of Papua (UNIPA), Manokwari, Indonesia, Pakuan University, Bogor, Indonesia Program Studi : PENDIDIKAN BIOLOGI
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