Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Siti Warnasih, Supriatno Salam, Uswatun Hasanah, Laksmi Ambarsari, Purwantiningsih Sugita

Judul : Total phenolic, flavonoid content and metabolite profiling of methanol extract of date (Phoenix dactylifera) seeds by LCQTOF- MS

Abstrak :
Phoenix dactylifera L. (date palm) has been used in traditional medicine to treat illnesses. Extracts of P.
dactylifera are reported to possess valuable pharmacological attributes. Date seeds are part of the palm trees that have notbeen widely used, based on previous studies that date seeds contain secondary metabolites such as phenolic acids
compounds, flavonoids, carotenoids, sterols, terpenoids and anthocyanin. The aim of this study was to identify the total
phenolic and flavonoids content from methanol extract of date seeds and characterize their metabolite profiles using the
LC-QTOF-MS instrument. Date seeds were extracted using methanol 96% by maceration method so that thick extracts
were obtained which were then analysed for the total phenolic and flavonoids content using UV-Vis spectrophotometer
instruments, while the profiles of secondary metabolites used LC-QTOF-MS instruments. The results of the analysis of
total phenolic and flavonoid of methanol extract of date seeds were 11.11 ± 0.71 g GAE/100 g and 2.15 ± 0.28 g QE/100
g, LC-QTOF-MS, it was concluded that the majority of date seed methanol extract contained phenolic acids compounds,
flavonoids, carotenoids and sterols.

Tahun : 2020 Media Publikasi : Prosiding

Kategori : Prosiding No/Vol/Tahun : 030029 / 2243 / 2020

ISSN/ISBN : -

PTN/S : Universitas Pakuan Program Studi : KIMIA

Bibliography :
1. I. M. Abu-Reidah, A. Gil-Izquierdo, S. Medina, and F. Ferreres, Food Res. Int. 100, 494-500 (2016).
2. M. Al- Farsi, C. Alasalvar, A. Morris, M. Baron, and F. Shahidi, J. Agric. Food Chem. 53, 7586−7591 (2005).
3. M. Al-Farsi, C. Alasalvar, M. Al-Abid, K. Al-Shoaily, M. Al-Amry, and F. Al-Rawahy, Food Chemistry 104 (3),
943–947 (2007).
4. F. M. Allouche, S. Touati, K. Mnafgui, N. Gharsallah, A. El Feki, and N. Allouche, Journal of Pharmacognosy
and Phytochemistr, 5 (3), 15-22 (2016)
5. M. S. Baliga, B. R. V. Baliga, S. M Kandathil, H. P. Bhat, And P. K. Vayalil, Food Research International 44,
1812–1822 (2011).
6. H. Boudries, P. Kefalas, and D. Hornero-Méndez, Food Chem. 101, 1372−1377 (2007).
7. C. C. Chang, M. H. Yang, H. M. Wen, and J. C. Chem, Journal of Food and Drug Analysis 10 (3), 178-182
(2002).
8. B. J. Harborne and H. Baxter Phytochemical Dictionary: A Handbook of Bioactive Compounds from Plants
(Taylor & Francis, London, 1993), pp. 791.
9. S. Hiroyuki, Y. Honda, S. Nakagawa, H. Ashida, and K. Kanazawa, J. Agric. Food Chem. 51, 571–581 (2003).
10. Y. J. Hong, F. A. Tomas-Barberan, A. A. Kader, and A. E. Mitchell, J. Agric. Food Chem. 54, 2405−2411
(2006).
11. J. Javanmardi, C. Stushnoff, E. Locke, and J. M. Vivanco, Food Chemistry 83, 547-550 (2003).
12. I. Khlif I, K. Jellali, T. Michel, M. Halabalaki, A. L. Skaltsounis, and N. Allouche, J. Chem. 2015, 1-11 (2015).
13. N. Kikuchi and T. Miki, Mikrochim. Acta 69, 89−96 (1978).
14. L. Long-Ze, H. Xian-Guo, M. Lindenmaier, J. Yang, M. Cleary, Q. Sheng-Xiang, and G. A. Cordell, J. Agric.
Food Chem. 48, 354–365 (2000).
15. A. Mansouri, G. Embarek, E. Kokkalouc, and P. Kefalas, Food Chem. 89, 411−420 (2005).
16. S. A. Mohamed, M. A. Awad, and A. D. Al-Qurashi, Scientia Horticulturae 167, 91-99 (2014).
17. M. U. Nasir, S. Hussain, S. Jabbar, F. Rashid, N. Khalid, and A. Mehmood, Science Letters 3, 17-22 (2015).
18. F. Nianbai, S. Yu, and R. L. Prior, J. Agric. Food Chem. 50, 3579–3585 (2002).
19. C. T. Yeh, L. C. Ching, and G. C. Yen, The Journal of Nutritional Biochemistry 20, 163−171 (2008).
20. L. Birosová, M. Mikulásová, and S. Vaverková, Biomedical papers of the Medical Faculty of the University
Palacký, Olomouc, Czechoslovakia 149, 489−491 (2005).
21. A. R. Tapas, A. M. Sakarkar, and R. B. Kakde, Tropical Journal of Pharmaceutical Research 7, 1089−1099
(2008).
22. Orhan, D. D. Tapas, B. Ozçelik, S. Ozgen, and F. Ergun, Microbiological Research. 165, 496-504 (2010)
23. B. Ozçelik, I. Orhan, and G. Toker, Journal of Biosciences 61, 632−638 (2006).
24. A. Gescher, Journal of Chemotherapy 16, 3−6 (2004).
25. D. B. Uteshev, E. B. Kostriukov, A. A. Karabinenko, V. L. Kovaleva, O. V. Makarova, and G. I. Storozhakov,
Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia 2, 19−22 (2000).
26. K. S. Mota, G. E. Dias, M. E. Pinto, A. Luiz-Ferreira, A. R. Souza-Brito, C. A. Hiruma-Lima,J. M. Barbosa-
Filho, and L. M. Batista, Molecules 14, 979−1012 (2009).
27. M. Xiao, Z. Yang, M. Jiu, J. You, and R. Xiao, Hua Xi Yi Ke Da Xue Xue Bao 23, 98−101 (1992).

28. S. Lafay, E. Gueux, Y. Rayssiguier, A. Mazur, C. Rémésy, and A. Scalbert, International Journal for Vitamin
and Nutrition Research 75, 119−125 (2005).
29. N. C. Wong, The Canadian Journal of Cardiology 17, 715−721 (2001).
30. C. Auger, P. L. Teissedre, P. Gérain, N. Lequeux, A. Bornet, S. Serisier, P. Besançon, B. Caporiccio, J. P.
Cristol, and J. M. Rouanet, Journal of Agricultural and Food Chemistry 53, 2015−2021 (2005).
31. C. Kamada, E. L. Da Silva, M. Ohnishi-Kameyama, J. H. Moon, and J. Terao, Free Radical Research 39,
185−194 (2005).
32. S. Dhingra and M. P. Bansal, Lipids in Health and Disease 5 (4), 28 (2005).
33. S. Dhingra and M. P. Bansal, Chemico-biological Interactions 161, 49−56 (2006).
34. H. D. Kaur and M. P. Bansal, Lipids in Health and Disease 16 (8), 55 (2009).
35. M. Srinivasan, R. Rukkumani, A. R. Sudheer, and V. P. Menon, Fundamental and Clinical Pharmacology 19,
491−496 (2005).
36. K. H. Janbaz, S. A. Saeed, and A. H. Gilani, Phytomedicine 11, 424−430 (2005).
37. W. F. Seifert, A. Bosma, H. F. Hendriks, R. E. van Leeuwen, G. C. and van Thiel-de Ruiter, I. Seifert-Bock, D.
L. Knook, and A. Brouwer, Liver 15, 1−8 (1995).
38. Q. S, Zheng, X. L. Sun, B. Xu, G. Li, and M. Song, Biomedical and Environmental Sciences 18, 65−70 (2005).
39. R. DomitroviÄ‡, H. Jakovac, D. GrebiÄ‡, C. Milin, and B. RadoseviÄ‡-StasiÄ‡, Biological Trace Element Research
126, 176−185 (2008).
40. I. T. Abdel-Raheem, A. A. Abdel-Ghany, and G. A. Mohamed, Biological & Pharmaceutical Bulletin 32, 61−67
(2009)
41. B. P. Chew, Journal of Dairy Science 76, 2804−2811 (1993).
42. P. Akbay, A. A. Basaran, U. Undeger, and N. Basaran, N, Phytotherapy Research, 17, 34−37 (2003).

URL : https://doi.org/10.1063/5.0001436

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Detail Karya Ilmiah Dosen

Siti Warnasih, Supriatno Salam, Uswatun Hasanah, Laksmi Ambarsari, Purwantiningsih Sugita

Judul	:	Total phenolic, flavonoid content and metabolite profiling of methanol extract of date (Phoenix dactylifera) seeds by LCQTOF- MS
Abstrak	:	Phoenix dactylifera L. (date palm) has been used in traditional medicine to treat illnesses. Extracts of P. dactylifera are reported to possess valuable pharmacological attributes. Date seeds are part of the palm trees that have notbeen widely used, based on previous studies that date seeds contain secondary metabolites such as phenolic acids compounds, flavonoids, carotenoids, sterols, terpenoids and anthocyanin. The aim of this study was to identify the total phenolic and flavonoids content from methanol extract of date seeds and characterize their metabolite profiles using the LC-QTOF-MS instrument. Date seeds were extracted using methanol 96% by maceration method so that thick extracts were obtained which were then analysed for the total phenolic and flavonoids content using UV-Vis spectrophotometer instruments, while the profiles of secondary metabolites used LC-QTOF-MS instruments. The results of the analysis of total phenolic and flavonoid of methanol extract of date seeds were 11.11 ± 0.71 g GAE/100 g and 2.15 ± 0.28 g QE/100 g, LC-QTOF-MS, it was concluded that the majority of date seed methanol extract contained phenolic acids compounds, flavonoids, carotenoids and sterols.
Tahun	:	2020	Media Publikasi	:	Prosiding
Kategori	:	Prosiding	No/Vol/Tahun	:	030029 / 2243 / 2020
ISSN/ISBN	:	-
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	1. I. M. Abu-Reidah, A. Gil-Izquierdo, S. Medina, and F. Ferreres, Food Res. Int. 100, 494-500 (2016). 2. M. Al- Farsi, C. Alasalvar, A. Morris, M. Baron, and F. Shahidi, J. Agric. Food Chem. 53, 7586−7591 (2005). 3. M. Al-Farsi, C. Alasalvar, M. Al-Abid, K. Al-Shoaily, M. Al-Amry, and F. Al-Rawahy, Food Chemistry 104 (3), 943–947 (2007). 4. F. M. Allouche, S. Touati, K. Mnafgui, N. Gharsallah, A. El Feki, and N. Allouche, Journal of Pharmacognosy and Phytochemistr, 5 (3), 15-22 (2016) 5. M. S. Baliga, B. R. V. Baliga, S. M Kandathil, H. P. Bhat, And P. K. Vayalil, Food Research International 44, 1812–1822 (2011). 6. H. Boudries, P. Kefalas, and D. Hornero-Méndez, Food Chem. 101, 1372−1377 (2007). 7. C. C. Chang, M. H. Yang, H. M. Wen, and J. C. Chem, Journal of Food and Drug Analysis 10 (3), 178-182 (2002). 8. B. J. Harborne and H. Baxter Phytochemical Dictionary: A Handbook of Bioactive Compounds from Plants (Taylor & Francis, London, 1993), pp. 791. 9. S. Hiroyuki, Y. Honda, S. Nakagawa, H. Ashida, and K. Kanazawa, J. Agric. Food Chem. 51, 571–581 (2003). 10. Y. J. Hong, F. A. Tomas-Barberan, A. A. Kader, and A. E. Mitchell, J. Agric. Food Chem. 54, 2405−2411 (2006). 11. J. Javanmardi, C. Stushnoff, E. Locke, and J. M. Vivanco, Food Chemistry 83, 547-550 (2003). 12. I. Khlif I, K. Jellali, T. Michel, M. Halabalaki, A. L. Skaltsounis, and N. Allouche, J. Chem. 2015, 1-11 (2015). 13. N. Kikuchi and T. Miki, Mikrochim. Acta 69, 89−96 (1978). 14. L. Long-Ze, H. Xian-Guo, M. Lindenmaier, J. Yang, M. Cleary, Q. Sheng-Xiang, and G. A. Cordell, J. Agric. Food Chem. 48, 354–365 (2000). 15. A. Mansouri, G. Embarek, E. Kokkalouc, and P. Kefalas, Food Chem. 89, 411−420 (2005). 16. S. A. Mohamed, M. A. Awad, and A. D. Al-Qurashi, Scientia Horticulturae 167, 91-99 (2014). 17. M. U. Nasir, S. Hussain, S. Jabbar, F. Rashid, N. Khalid, and A. Mehmood, Science Letters 3, 17-22 (2015). 18. F. Nianbai, S. Yu, and R. L. Prior, J. Agric. Food Chem. 50, 3579–3585 (2002). 19. C. T. Yeh, L. C. Ching, and G. C. Yen, The Journal of Nutritional Biochemistry 20, 163−171 (2008). 20. L. Birosová, M. Mikulásová, and S. Vaverková, Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia 149, 489−491 (2005). 21. A. R. Tapas, A. M. Sakarkar, and R. B. Kakde, Tropical Journal of Pharmaceutical Research 7, 1089−1099 (2008). 22. Orhan, D. D. Tapas, B. Ozçelik, S. Ozgen, and F. Ergun, Microbiological Research. 165, 496-504 (2010) 23. B. Ozçelik, I. Orhan, and G. Toker, Journal of Biosciences 61, 632−638 (2006). 24. A. Gescher, Journal of Chemotherapy 16, 3−6 (2004). 25. D. B. Uteshev, E. B. Kostriukov, A. A. Karabinenko, V. L. Kovaleva, O. V. Makarova, and G. I. Storozhakov, Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia 2, 19−22 (2000). 26. K. S. Mota, G. E. Dias, M. E. Pinto, A. Luiz-Ferreira, A. R. Souza-Brito, C. A. Hiruma-Lima,J. M. Barbosa- Filho, and L. M. Batista, Molecules 14, 979−1012 (2009). 27. M. Xiao, Z. Yang, M. Jiu, J. You, and R. Xiao, Hua Xi Yi Ke Da Xue Xue Bao 23, 98−101 (1992). 28. S. Lafay, E. Gueux, Y. Rayssiguier, A. Mazur, C. Rémésy, and A. Scalbert, International Journal for Vitamin and Nutrition Research 75, 119−125 (2005). 29. N. C. Wong, The Canadian Journal of Cardiology 17, 715−721 (2001). 30. C. Auger, P. L. Teissedre, P. Gérain, N. Lequeux, A. Bornet, S. Serisier, P. Besançon, B. Caporiccio, J. P. Cristol, and J. M. Rouanet, Journal of Agricultural and Food Chemistry 53, 2015−2021 (2005). 31. C. Kamada, E. L. Da Silva, M. Ohnishi-Kameyama, J. H. Moon, and J. Terao, Free Radical Research 39, 185−194 (2005). 32. S. Dhingra and M. P. Bansal, Lipids in Health and Disease 5 (4), 28 (2005). 33. S. Dhingra and M. P. Bansal, Chemico-biological Interactions 161, 49−56 (2006). 34. H. D. Kaur and M. P. Bansal, Lipids in Health and Disease 16 (8), 55 (2009). 35. M. Srinivasan, R. Rukkumani, A. R. Sudheer, and V. P. Menon, Fundamental and Clinical Pharmacology 19, 491−496 (2005). 36. K. H. Janbaz, S. A. Saeed, and A. H. Gilani, Phytomedicine 11, 424−430 (2005). 37. W. F. Seifert, A. Bosma, H. F. Hendriks, R. E. van Leeuwen, G. C. and van Thiel-de Ruiter, I. Seifert-Bock, D. L. Knook, and A. Brouwer, Liver 15, 1−8 (1995). 38. Q. S, Zheng, X. L. Sun, B. Xu, G. Li, and M. Song, Biomedical and Environmental Sciences 18, 65−70 (2005). 39. R. DomitroviÄ‡, H. Jakovac, D. GrebiÄ‡, C. Milin, and B. RadoseviÄ‡-StasiÄ‡, Biological Trace Element Research 126, 176−185 (2008). 40. I. T. Abdel-Raheem, A. A. Abdel-Ghany, and G. A. Mohamed, Biological & Pharmaceutical Bulletin 32, 61−67 (2009) 41. B. P. Chew, Journal of Dairy Science 76, 2804−2811 (1993). 42. P. Akbay, A. A. Basaran, U. Undeger, and N. Basaran, N, Phytotherapy Research, 17, 34−37 (2003).
URL	:	https://doi.org/10.1063/5.0001436