Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Barahima Abbas1, Muhammad Dailami2, Budi Santoso3, Munarti4

Judul : Genetic Variation of Sago Palm (Metroxylonsagu Rottb.) Progenies with Natural Pollination by Using RAPD Markers
Abstrak :

Sago palm is flowering and fruiting just once in their life cycle. Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the starch contained in their trunk. Sago palm pollination may occur as self-pollinated and cross-pollinated. If cross-pollinated was occurred in the pollination process, it will be varied of their progenies. This study aims to reveal the genetic variation of sago palms progenies with naturally pollinated process. The research method is to collect seeds from one parent trees that have produced ripe fruit. Fruit seeds germinated to be made and tested genetic variation using RAPD markers. Iso-lation of DNA is done by using the fresh young leaves. DNA amplification is done by using RAPD primers. The results showed that the progenies derived from naturally pollinated of sago palms were genetically varied based on RAPD markers and also varied based on morphological phenotypic. Variations occurred in the progenies of sago palm indicated that the sago palms were estimated cross-pollinated naturally, as a result fruits and seeds with genetically differences.

Tahun : 2017 Media Publikasi : Jurnal Internasional
Kategori : Jurnal No/Vol/Tahun : 4 / 9 / 2017
ISSN/ISBN : ISSN Online 2150-4105
PTN/S : University of Papua (UNIPA), Manokwari, Indonesia, Pakuan University, Bogor, Indonesia Program Studi : PENDIDIKAN BIOLOGI
Bibliography :

1.Ehara, H. (2012) Potency of Sago Palm as a Carbohydrate Resource for Strengthening the Food Security Pro-gram. Japanese Studies Journal, Special Issue, 11-21.
2. Anugoolprasert, O., Ehara, H. and Nato, H. (2014) Growth Response and Nutrient Concentrations of Sago Palm under Aluminium Stress. Thammasat International Journal of Science and Technology, 19, 37-63.
3. Karim, A.A., Pei-Lang Tie, A., Manan, D.M.A. and Zaidul, I.S.M. (2008) Starch from the Sago (Metroxylonsagu) Palm Tree—Properties, Prospects and Challenges as a Source for Food and Other Uses. Comprehensive Reviews in Food Science and Food Safety, 7, 215-228.
4. Bujang, K.B. (2008) Potential of Bioenergy from the Sago Industries in Malaysia. Biotechnology, 14, 8 p.
5. Kanro, M.Z., Rouw, A., Widjono, A., Syamsuddin, Amisnaipa and Atekan (2003) Utilization of Sago Palm in West Papua Province. Jurnal Litbang Pertanian, 22, 116-124. (In Indonesia language)
6. Mofu, S.S. and Abbas. B. (2015) Development of Sago Palm Research and Agroindustry in University of Papua. The 12th International Sago Symposium, Tokyo, 15-17 September 2015.
7. Sofiari, E. and Kirana, R. (2009) Analysis of Segregation Pattern and Distribution of Several Pepper Characteris-tic Analisis Pola Segregasidan. J. Hort., 19, 255-253. (In Indonesia language)
8. Flach, M. (1997) Sago Palm Metroxylonsagu Rottb. Promoting the Conservation and Used of Unther Utilized and Neglected Crops. 13. Institute of Plant Genetics and Crop Plant Research, Gatersleben/Internasional Plant Genetic Resources Institute (IPGRI), Rome.
9. Powel, W., Castillo, C.O., Chaluers, K.J., Provan, J. and Waugh, R. (1995) Polymerase Chain Reaction Based- Assays for the Characterization of Plant Genetic Resources. Electrophoresis, 16, 1726-1730.

10. Colombo, C., Second, G., Valle, T.L. and Charrier, A. (1998) Genetic Diversity Characterization of Cassava Cul-tivars (Manihotesculenta Cranz.) RAPD Markers. Genetics and Molecular Biology, 21, 69-84.
11. Ferdinandez, Y.S.N., Somers, D.J. and Coulman, B.E. (2001) Estimating the Genetic Relationship of Hybrid Bromegrass to Smooth Bromegrass and Medow Bromegrass Using RAPD Markers. Plant Breeding, 120, 149-153.
12. Sneath, P.H. and Sokal, R.R. (1973) Numerical Taxonomy. Freeman, San Francisco, 573 p.
13. Rohlf, F.J. (1998) NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, Version 2.02. Exeter Software, New York.
14. Abbas, B., Bintoro, M.H., Sudarsono, Surahman, M. and Ehara, H. (2009) Genetic Relationship of Sago Palm (Metroxylon sagu Rottb.) in Indonesia Based on RAPD Markers. Journal of Biological Diversity, 10, 168-174.
15. Ehara, H., Kosaka, S., Shimura, N., Matoyama, D., Morita, O., Naito, H., Mizota, C., Susanto, S., Bintoro, M.H. and Yamamotom, Y. (2003) Relationship between Geographical Distribution and Genetic Distance of Sago Palm in Malay Archipelago. Sago Palm, 11, 8-13.
16. Abbas, B., Bintoro, M.H., Surahman, M. and Ehara, H. (2009) Genetic Relationship of Sago Palm (Metroxylon sagu Rottb.) in Indonesia Based on RAPD Markers. Journal of Biological Diversity, 10, 168-174.
17. Abbas, B., Renwarin, Y., Bintoro, M.H., Surahman, M. and Ehara, H. (2010) Genetic Diversity of Sago Palm in Indonesia Based on Chloroplast DNA (cpDNA) Markers. Journal of Biological Diversity, 11, 112-117.
18. Abbas, B. and Ehara, H. (2012) Assessment Genetic Variation and Relationship of Sago Palm (Metroxylon sagu Rottb.) in Indonesia Based on Specific Expression Gene (Wx Genes) Markers. African Journal of Plant Science, 6, 314-320.
19. Dewi, R.K., Bintoro, M.H. and Sudrajat. (2016) Morphological Characteristic and Yield Potential of Sago Palms (Metroxylon spp.) Accession in South Sorong Regency, West Papua. Jurnal Agronomi Indonesia, 44, 91-97.
20. Jong, F.S. (2011) Growth and Yield Parameters of Natural Sago Forest for Commersial Operations. The 10th In-ternational Sago Symposium, Bogor-Indonesia, 29-31 October 2011.
21. Jong, F.S. (1995) Research for the Development of Sago Palm (Metroxylon saguRottb.) Cultivation in Sarawak, Malaysia. Department of Agriculture, Kuching, Sarawak, Malaysia.