Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Ade Heri Mulyati, Diana Widiastuti, Eka Herlina, Siti Warnasih, Yudhie Suchyadi

Judul : Cytotoxic Effects of Cassava (Manihot esculenta Crantz), Adira-2, Karikil and Sao Pedro Petro Varieties against P-388 Murine Leukemia Cells

Abstrak :
This research examines the cytotoxic effect of cassava (Manihot esculenta Crantzs) in inhibiting growth of P- 388 murine leukemia cancer cells. This study uses three cultivar of cassava samples from West Java Indonesia including Adira-2, from Cimahpar Bogor, Karikil from Cililin West Bandung and Sao Pedro Petro from Cisarua, Bogor. Cassava was first extracted with ethanol at room temperature, which was then
partitioned succesively with n-hexane, ethyl acetate and n-butanol. All of the extracts were evaluated against P-388 murine leukemia cells in-vitro using MTT assay. As a result, the n-hexane extract of San Pedro Petro cassava from
Cisarua Bogor has an inhibition concentration (IC50) value of 15.8µg/mL which can prevent the growth of murine leukemia P-388 cancer cells.

Tahun : 2018 Media Publikasi : Jurnal Internasional

Kategori : Jurnal No/Vol/Tahun : II / 22 / 2018

ISSN/ISBN : -

PTN/S : Universitas Pakuan Program Studi : KIMIA

Bibliography :
1. Djazuli M. and Bradbury J.H., Cyanogen content of cassava
roots and flour in Indonesia, Food Chemistry, 65(4), 523-525
(1999)
2. Hidayat A., Zuraida N. and Hanarida I., The cyanogenic
potential of roots and leaves of ninety-nine cassava cultivars,
Indonesian Journal of Agricultural Science, 3(1), 25-32 (2002)
3. Bradbury M.G., Egan S.V. and Bradbury J.H., Picrate paper kits
for determination of total cyanogens in cassava roots and all forms
of cyanogens in cassava products, Journal of the Science of Food
and Agriculture, 79(4), 593-601 (1999)
4. Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski
M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H. and
Boyd M.R., Feasibility of drug screening with panels of human
tumor cell lines using a microculture tetrazolium assay, Cancer
Research, 48(3), 589-601 (1988)
5. Allen L.V. and Ansel H.C., Ansel's pharmaceutical dosage
forms and drug delivery systems, 10th ed., Lippincott Williams &
Wilkins, Philadelphia (2014).

URL : https://worldresearchersassociations.com/SpecialIssueAugust2018.aspx

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Detail Karya Ilmiah Dosen

Ade Heri Mulyati, Diana Widiastuti, Eka Herlina, Siti Warnasih, Yudhie Suchyadi

Judul	:	Cytotoxic Effects of Cassava (Manihot esculenta Crantz), Adira-2, Karikil and Sao Pedro Petro Varieties against P-388 Murine Leukemia Cells
Abstrak	:	This research examines the cytotoxic effect of cassava (Manihot esculenta Crantzs) in inhibiting growth of P- 388 murine leukemia cancer cells. This study uses three cultivar of cassava samples from West Java Indonesia including Adira-2, from Cimahpar Bogor, Karikil from Cililin West Bandung and Sao Pedro Petro from Cisarua, Bogor. Cassava was first extracted with ethanol at room temperature, which was then partitioned succesively with n-hexane, ethyl acetate and n-butanol. All of the extracts were evaluated against P-388 murine leukemia cells in-vitro using MTT assay. As a result, the n-hexane extract of San Pedro Petro cassava from Cisarua Bogor has an inhibition concentration (IC50) value of 15.8µg/mL which can prevent the growth of murine leukemia P-388 cancer cells.
Tahun	:	2018	Media Publikasi	:	Jurnal Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	II / 22 / 2018
ISSN/ISBN	:	-
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	1. Djazuli M. and Bradbury J.H., Cyanogen content of cassava roots and flour in Indonesia, Food Chemistry, 65(4), 523-525 (1999) 2. Hidayat A., Zuraida N. and Hanarida I., The cyanogenic potential of roots and leaves of ninety-nine cassava cultivars, Indonesian Journal of Agricultural Science, 3(1), 25-32 (2002) 3. Bradbury M.G., Egan S.V. and Bradbury J.H., Picrate paper kits for determination of total cyanogens in cassava roots and all forms of cyanogens in cassava products, Journal of the Science of Food and Agriculture, 79(4), 593-601 (1999) 4. Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H. and Boyd M.R., Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay, Cancer Research, 48(3), 589-601 (1988) 5. Allen L.V. and Ansel H.C., Ansel's pharmaceutical dosage forms and drug delivery systems, 10th ed., Lippincott Williams & Wilkins, Philadelphia (2014).
URL	:	https://worldresearchersassociations.com/SpecialIssueAugust2018.aspx