| Abstrak |
: |
Pomelo leaves (Citrus maxima) contain secondary metabolites i.e. flavonoids and
saponins. Based on several studies, flavonoids and saponins can act as antibacterial.
Based on these indications, it is important to do research on the antibacterial potential
of pomelo leaf extract (Citrus maxima) against Shigella dysenteriae bacteria. This
study aims to determine the antibacterial activity of 96% ethanol extract of pomelo
leaves as a result of maceration and reflux methods to Shigella dysenteriae and
determine the total flavonoid content of 96% ethanol extract of pomelo leaves as a
result of maceration and reflux methods. This study was an experimental study by
determining the total flavonoid content of pomelo leaf extract and testing the
antibacterial activity of pomelo leaf extract resulting from maceration and reflux as
measured by the Minimum Inhibitory Concentration (MIC) and Zone of Inhibition (ZOI)
against Shigella dysenteriae bacteria. The results of the study it was obtained 89.9 g of
reflux extract with a yield of 17.98±0.39% and maceration yield of 95.45 g with a yield of
19.09±0.49%. Flavonoid content of pomelo leaf extract from reflux was 1.49±0.01%
while maceration yield was 2.28±0.10%. Pomelo leaf extract from reflux and
maceration did not provide inhibition at concentrations of 5% to 30%, the results
showed that MIC from both extraction methods was at a concentration of 35%. The
highest ZOI was found in the 45% which was 10.92±0.08 mm on the results of the reflux
method and 9.15±0.03 mm on the results of the maceration method. The conclusion of
this study is that the extract from reflux results in greater ZOI and biggest of flavonoids
content.
Keywords: Citrus maxima, flavonoids, maceration, reflux, Shigella dysenteriae
|
| Bibliography |
: |
DAFTAR PUSTAKA
Aiello, Susan E. 2012. The Merck Etinary Manual.
Merck Sharp & Dohme Corp. USA.
Assidqi, Khoirunnisa, Wahyu T., dan Setyawati S.
2012. Potensi Ekstrak Daun Patikan
K e b o ( E u p h o r b i a h i r t a ) S e b a g a i
Antibakteri Terhadap Aeromonas
hydrophila Secara In Vitro. Journal of
Marine and Coastal Science. Vol. 1 No. 2.
Hal. 117.
Azizah, Nur, Afghani J., dan Harlia. 2015.
Aktivitas Anti Rayap Ekstrak Daun Jeruk
Bali (Citrus maxima (Burm.) Merr.)
Terhadap Rayap Tanah Coptotermes sp.
Jurnal Kimia Khatulistiwa. Vol. 4. No. 3.
Hal. 33-39.
Black, Joyce M. dan Jacobs E. M. 1993. Medical
Surgical Nursing (4th Edition). W.B.
Saunders Company. Philadelphia.
Chang, Chia C., Yang Ming H., Wen Hwei M.,
Chern Jiing C. 2002. Estimationof Total
Flavonoid Content in Propolis by Two
Complementary Colorimetric Methods.
Journal of Food and Drug Analysis. Vol.
10. Hal. 178-182.
Departemen Kesehatan Republik Indonesia.
1986. Sediaan Galenik. Jakarta.
Departemen Kesehatan Republik
Indonesia.
Departemen Kesehatan Republik Indonesia.
2000. Parameter Standar Umum Ekstrak
Tumbuhan Obat. Jakarta. Dirjen POM.
Dwidjoseputro. 1988. Pengantar Fisiologi
Tumbuhan. Jakarta. Erlangga.
Hanani, Endang. 2015. AnalisisFitokimia.
Jakarta. Buku Kedokteran EGC.
Haeria, Nurshalati T., dan Munadiah. 2018.
Penentuan Kadar Flavonoid Dan
Kapasitas Antioksidan Ekstrak Etanol
Kulit Batang Kelor (Moringa oleifera L)
dengan Metode DPPH, CUPRAC Dan
F R A P. J u r n a l F a r m a s i F a k u l t a s
Kedokteran dan Ilmu Kesehatan UINAM.
Vol.6. No.2. Hal90-96.
Hadioetomo, Ratna S. 1985. Mikrobiologi Dasardasar
Praktik. Gramedia. Jakarta.
Munaari, Chaleb, Edi S., dan Julius P. 2014.
Aktivitas Penangkal Radikal Hidroksil
Fraksi Flavonoid dari Limbah Tongkol
Jagung Pada Tikus Wistar. Jurnal
MIPAUNSRAT Online. Vol. 3. No. 2. Hal.
136.
Munfaati, Putri N., Ratnasari, E., dan Trimulyono,
G. 2015. Aktivitas Senyawa Antibakteri
Ekstrak Herba Meniran (Phyllanthus
niruri) Terhadap Pertumbuhan Bakteri
Shigella dysenteriae Secara in Vitro.
LenteraBio. Vol. 4. No. 1. Hal. 64-65.
P e r m a d i , A fi f , S u t a n t o , S r i W. 2 0 1 8 .
P e r b a n d i n g a n M e t o d e E k s t r a k s i
Bertingkat Dan Tidak Bertingkat
Terhadap Flavonoid Total Herba Ciplukan
( P h y s a l i s a n g u l a t a L . ) S e c a r a
Kolorimetri. Jurnal Online Mahasiswa
(JOM) Bidang Farmasi. Vol. 1. No. 1 . Hal.
8.
Reynolds, James. E. F. 1996. Martindale, The
th Extra Pharmacopeia 31 Edition. The
Royal Pharmaceutical. Society Press.
London.
Saifudin, Aziz, Viesa R., dan Hilwan Y. T. 2011.
S t a n d a r i s a s i B a h a n O b a t A l a m .
Yogyakarta. Graha Ilmu.
Suhardiman, Ari, Dadang J., Maryzka D. 2018. Uji
A n t i b a k t e r i R i m p a n g G a n d a s u l i
(Hedychium coronarium) Terhadap
Bakteri Staphylococcus Aureus dan
Escherichia coli dengan Perbandingan
M e t o d e E k s t r a k s i . J o u r n a l o f
Pharmacopolium. Vol. 1. No. 2. Hal.62-
|
@official_unpak
@unpak
@unpak
UNPAK TV