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Detail Karya Ilmiah Dosen

Tri Saptari Haryani, Bina Lohitasari,Triastinurmiatiningsih

Judul : Toxicity and Compound Identification of PadinaaustralisExtract

Abstrak :
Abstract: People awareness of the importance of health has increased significantly in the last decade, it forces people to find alternative treatments which are cheaper and safer when compared with the use of synthetic drugs. Research on antibacterial activity of Padina australis against Escherichia coli have been conducted, but it does not identified yet which active compounds in the Padina australis extract that has potential as an antibacterial. Identification phytol compounds from the extracts of Padina australis and toxicity tests have been conducted using BSLT and GCMS methods. The results of this research identify that Padina australis extract with ethanol has antibacterial activity value of LC50177.83 ppm. Phytochemical test results show that active ingredient of Padina australis extract is terpenoid compounds, and that triterpenoids has potential as an antibacterial. GC-MS test result shows that active compound of Padina australis extract consists of phytol compound which has 90-99% similarity with steroids, phenols, fatty acids, carboxylic acids, hydrocarbons, and proteins. Compound identification test of active Padina australis extracts through GC-MS methods show that phytol compounds are useful as antibacterial and the toxicity test results show that phytol compounds are not cytotoxic.

Index Terms: Antibacterial, Extract Padina Australis, LC50.

Tahun : 2019 Media Publikasi : Jurnal Internasional

Kategori : Jurnal No/Vol/Tahun : - / Volume-8 Issue-2S7 / 2019

ISSN/ISBN : 2277-3878

PTN/S : Universitas Pakuan Program Studi : BIOLOGI

Bibliography :
L. Goldberg,. Functional Food,Designer Food, Pharma Food, Neutraceuticals. New York: Chapman and Hall, 1984.2.A. Rasyid. "Beberapa catatan tentang alginat."Osena, vol. 30, no. 1, pp. 9-14, 2005. 3.T. S. Haryaniand Triastinurmiatiningsih. Pengembangan Formula Tablet Ekstrak Padina austra-lis Sebagai Antibakteri Escherichia coli Penyebab Penyakit Diare. Report Research, Jakarta: Kemenristek Dikti, 2014.4.A. Susanto, Kandungan Sumber Daya Hayati Alga Coklat. Jakarta: Gramedia, 2008.5.K. N. Kim, S. J., Heo, W. J.Yoon, S. M.Kang, G.Ahn, T. H. Yi, and Y. J. Jeon. “Fucoxanthin in-hibits the inflammatory response by suppressing the activation of NF-κB and MAPKs in lipopolysac-charide-induced RAW 264.7 macrophages.”European Journal Pharmacology,vol. 649, no. 1, pp. 369-375, 2010.6.L. Limantaraand H.Heriyanto, “Studi Komposisi Pigmen dan Kandungan Fukosantin Rumput Laut Cokelat dari Perairan Madura dengan Kromatogra Cair Kinerja Tinggi.”Ilmu Kelautan: Indonesian Journal of Marine Sciences,vol. 15, no. 1, pp. 23-32, 2012.7.A. V. Krishnaraju, T. V. N. Rao, D. Sundararaju, M. Vanisree, H. S. Tsay and G.V. Subbaraju. “Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay.”International Journal of Applied Science and Engineering, vol. 3,no.2, pp. 125-134, 2005.8.S. R. Tamat, T. Wikanta and L. S. Maulina. “Aktivitas Antioksidan dan Toksisitas Senyawa Bioak-tif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal.”Jurnal Ilmu Kefarmasian Indonesia, vol. 5, no. 1, pp. 31-36, 2007.9.D. Juniarti, Osmeli and Yuhernita. “Kandungan Senyawa Kimia, Uji Toksisitas, (Brine Shrimp Lethality Test) dan Antioksidan (1,1-diphenyl-2 pikrilhydrazyl) dari Ekstrak Daun Saga (Abrus preca-torius l.).”Makara Sains,vol. 13 , no. 1, pp. 50-54, 2009.10.J. Santoso, S. Anwariyah, R. O. Rumiantin, A. P. Putri, N. Ukhty andY. Yoshie-Stark. “Phenol Content, Antioxidant Activity and Fibers profile of Four Tropical Seagrasses from Indonesia.”Journal of Coastal Development, vol. 15,no. 2,pp. 189-196, 2012.11.S. V. Bhat, B. A. Nagasampagi and S. Meenakshi. Natural Product Chemistry and Application. New Delhi: NarosaPublishing House, 2009.12.J. B. Harborne. “Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan.”Bandung: Institut TeknologiBandung, 1987.13.B. N. Meyer, N. R. Ferrigni, J. E. Putman, L. B. Jacbsen, D. E. Nicols and J. L.McLaughlin. “Brine Shrimp: A Comvenient general Bioassay for Active Plant Constituents.”Planta Medica, vol. 45, no. 5, pp. 31-34, 1982

URL : https://www.ijrte.org/wp-content/uploads/papers/v8i2S7/B10160782S719.pdf

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Detail Karya Ilmiah Dosen

Tri Saptari Haryani, Bina Lohitasari,Triastinurmiatiningsih

Judul	:	Toxicity and Compound Identification of PadinaaustralisExtract
Abstrak	:	Abstract: People awareness of the importance of health has increased significantly in the last decade, it forces people to find alternative treatments which are cheaper and safer when compared with the use of synthetic drugs. Research on antibacterial activity of Padina australis against Escherichia coli have been conducted, but it does not identified yet which active compounds in the Padina australis extract that has potential as an antibacterial. Identification phytol compounds from the extracts of Padina australis and toxicity tests have been conducted using BSLT and GCMS methods. The results of this research identify that Padina australis extract with ethanol has antibacterial activity value of LC50177.83 ppm. Phytochemical test results show that active ingredient of Padina australis extract is terpenoid compounds, and that triterpenoids has potential as an antibacterial. GC-MS test result shows that active compound of Padina australis extract consists of phytol compound which has 90-99% similarity with steroids, phenols, fatty acids, carboxylic acids, hydrocarbons, and proteins. Compound identification test of active Padina australis extracts through GC-MS methods show that phytol compounds are useful as antibacterial and the toxicity test results show that phytol compounds are not cytotoxic. Index Terms: Antibacterial, Extract Padina Australis, LC50.
Tahun	:	2019	Media Publikasi	:	Jurnal Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	- / Volume-8 Issue-2S7 / 2019
ISSN/ISBN	:	2277-3878
PTN/S	:	Universitas Pakuan	Program Studi	:	BIOLOGI
Bibliography	:	L. Goldberg,. Functional Food,Designer Food, Pharma Food, Neutraceuticals. New York: Chapman and Hall, 1984.2.A. Rasyid. "Beberapa catatan tentang alginat."Osena, vol. 30, no. 1, pp. 9-14, 2005. 3.T. S. Haryaniand Triastinurmiatiningsih. Pengembangan Formula Tablet Ekstrak Padina austra-lis Sebagai Antibakteri Escherichia coli Penyebab Penyakit Diare. Report Research, Jakarta: Kemenristek Dikti, 2014.4.A. Susanto, Kandungan Sumber Daya Hayati Alga Coklat. Jakarta: Gramedia, 2008.5.K. N. Kim, S. J., Heo, W. J.Yoon, S. M.Kang, G.Ahn, T. H. Yi, and Y. J. Jeon. “Fucoxanthin in-hibits the inflammatory response by suppressing the activation of NF-κB and MAPKs in lipopolysac-charide-induced RAW 264.7 macrophages.”European Journal Pharmacology,vol. 649, no. 1, pp. 369-375, 2010.6.L. Limantaraand H.Heriyanto, “Studi Komposisi Pigmen dan Kandungan Fukosantin Rumput Laut Cokelat dari Perairan Madura dengan Kromatogra Cair Kinerja Tinggi.”Ilmu Kelautan: Indonesian Journal of Marine Sciences,vol. 15, no. 1, pp. 23-32, 2012.7.A. V. Krishnaraju, T. V. N. Rao, D. Sundararaju, M. Vanisree, H. S. Tsay and G.V. Subbaraju. “Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay.”International Journal of Applied Science and Engineering, vol. 3,no.2, pp. 125-134, 2005.8.S. R. Tamat, T. Wikanta and L. S. Maulina. “Aktivitas Antioksidan dan Toksisitas Senyawa Bioak-tif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal.”Jurnal Ilmu Kefarmasian Indonesia, vol. 5, no. 1, pp. 31-36, 2007.9.D. Juniarti, Osmeli and Yuhernita. “Kandungan Senyawa Kimia, Uji Toksisitas, (Brine Shrimp Lethality Test) dan Antioksidan (1,1-diphenyl-2 pikrilhydrazyl) dari Ekstrak Daun Saga (Abrus preca-torius l.).”Makara Sains,vol. 13 , no. 1, pp. 50-54, 2009.10.J. Santoso, S. Anwariyah, R. O. Rumiantin, A. P. Putri, N. Ukhty andY. Yoshie-Stark. “Phenol Content, Antioxidant Activity and Fibers profile of Four Tropical Seagrasses from Indonesia.”Journal of Coastal Development, vol. 15,no. 2,pp. 189-196, 2012.11.S. V. Bhat, B. A. Nagasampagi and S. Meenakshi. Natural Product Chemistry and Application. New Delhi: NarosaPublishing House, 2009.12.J. B. Harborne. “Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan.”Bandung: Institut TeknologiBandung, 1987.13.B. N. Meyer, N. R. Ferrigni, J. E. Putman, L. B. Jacbsen, D. E. Nicols and J. L.McLaughlin. “Brine Shrimp: A Comvenient general Bioassay for Active Plant Constituents.”Planta Medica, vol. 45, no. 5, pp. 31-34, 1982
URL	:	https://www.ijrte.org/wp-content/uploads/papers/v8i2S7/B10160782S719.pdf