Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Nina Herlina, Dwi Indriati

Judul : Study of Hypoglicemic Effect and Acute Toxicity of Ethyl Acetat Fraction Tofu Residue
Abstrak :

The study aimed to investigate oral glucose tolerance test (OGTT) and acute toxicity of Ethyl Acetat Fraction Tofu Residue (EAFTR) on DDY mice. Methods: EAFTR (200 mg/kg b.w) was prepared and administered orally to experimental animals used. The hypoglycemic effects of EAFTR on normal mice and orally glucose (3 g/kg bw) induced hyperglycemic mice were compared with aquadest, glibenclamide and dry tofu residue (200 mg/kg b.w). EAFTR was administered and blood glucose level was obtained by pricking the tail vein using glucometer at time -30, 0, 30, 60, 120  minutes. Hypoglicemic effect evaluated by Area Under Curve (AUC) measurement. The EAFTR was tested for acute toxicity in mice at a dose of 50, 500 and 2,000 mg/kg b.w. p.o once daily for 14 days. Results: The AUC of EAFTR treated groups were not different with control, Metformin treated and dry tofu residue. All doses of the EAFTR did not exert any sign or symptom of toxicity and the dead mice was not found. Conclusion: The findings of the present study can be concluded that EAFTR showed a hypoglicemic effect  and did not show toxic symptom until dose 2000 mg/kg b.w.


Keywords: Blood glucose, Hypoglicemic Effect, acute Toxicity, Tofu Residue, Diabetes

Tahun : 2019 Media Publikasi : Poster
Kategori : Poster No/Vol/Tahun : 0 / 0 / 0
PTN/S : Universitas Pakuan Program Studi : FARMASI
Bibliography :

  1. Messina M. Soy And Health Update: Evaluation Of The Clinical And Epidemiologic Literature. Nutrients. 2016 Nov 24;8(12). Pii: E754.
  2. Wu, Y., Ding, Y., Tanaka, Y., & Zhang, W. Risk Factors Contributing to Type 2 Diabetes and Recent Advances in the Treatment and Prevention. International Journal of Medical Sciences. 2014; 11(11), 1185–1200. doi:10.7150/ijms.10001 
  3. World Health Organization. Global report on diabetes. [document on the Internet]. Geneva; World Health Organization; 2016.  [cited 2019 Des 3]. Available from:
  4. Bo Li, Meiying Qiao & Fei Lu. Composition, Nutrition, and Utilization of Okara (Soybean Residue), Food Reviews International. 2012; 28:3, 231.252
  5. Das, D., Sarkar, S., Bordoloi, J., Wann, S. B., Kalita, J., & Manna, P. Daidzein, its effects on impaired glucose and lipid metabolism and vascular inflammation associated with type 2 diabetes. BioFactors. 2018; Sep;44(5):407-417.
  6. Cheong, S. H., Furuhashi, K., Ito, K., Nagaoka, M., Yonezawa, T., et al. Antihyperglycemic effect of equol, a daidzein derivative, in cultured L6 myocytes and ob/ob mice. Mol. Nutr. Food Res. 2014; 58, 267–277.
  7. Cheong, S. H., Furuhashi, K., Ito, K., Nagaoka, M., Yonezawa, T., et al. Daidzein promotes glucose uptake through glucose transporter 4 translocation to plasma membrane in L6 myocytes and improves glucose homeostasis in Type 2 diabetic model mice. J. Nutr. Biochem.2014; 25, 136–143
  8. Cao, Y. K., Zhang, S. F., Zou, S. E., and Xia, X. Daidzein improves insulin resistance in ovariectomized rats. Climacteric. 2013;16, 111–116.