Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Bina Lohita Sari, Triastinurmiatiningsih, Tri Saptari Haryani

Judul : Optimasi Metode Microwave-Assisted Extraction (MAE) untuk Menentukan Kadar Flavonoid Total Alga Coklat Padina australis

Abstrak :
Metode Microwave-Assisted Extraction (MAE) digunakan untuk mengekstraksi senyawa flavonoid dari simplisia alga coklat Padina australis. Sampel diperoleh dari Pantai Bayah, Banten Indonesia. Kondisi optimal ekstraksi ditentukan dengan Response Surface Methodology (RSM). Desain Box-Behnken (BBD) digunakan untuk mengevaluasi pengaruh 3 faktor dengan 3 level yaitu daya microwave (300, 450, 600 watt), konsentrasi etanol (30, 50, 70%), dan waktu ekstraksi (7, 8 dan 9 menit) dengan 15 perlakuan yang berbeda. Hasil disain eksperimen dengan BBD menunjukkan kondisi ekstraksi optimum yaitu pada daya microwave 414 watt, konsentrasi etanol 50,33% dan waktu ekstraksi 7,89 menit menghasilkan kadar prediksi flavonoid sebesar nilai 0,2963%. Nilai ini mendekati nilai kadar flavonoid yang diperoleh secara eksperimental sebesar 0,2961%, pada kondisi menggunakan daya microwave sebesar 450 watt, konsentrasi etanol 50% dengan lama ekstraksi 8 menit. Berdasarkan hasil penelitian, kadar flavonoid total dapat meningkat secara signifikan dengan melakukan optimasi proses MAE menggunakan RSM.
Kata kunci: Microwave-Assisted Extraction, kadar flavonoid total, alga coklat, Padina australis

Tahun : 2020 Media Publikasi : Jurnal Nasional Terakreditasi A

Kategori : Jurnal No/Vol/Tahun : 16 / 12 / 2020

ISSN/ISBN : 1412-4092/ e ISSN 2443-4183

PTN/S : universitas pakuan Program Studi : FARMASI

Bibliography :
Alara, O.R., Abdurahman, N.H., and Olalere, O.A., 2018. Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool. Journal of Food Measurement and Characterization 12, 1107-1122. doi: 10.1007/s11694-018-9726-3.
Bas, D., and Boyaci, I.H., 2007. Modeling and optimization I: Usability of response surface methodology. Journal of Food Engineering 78(3), 836-45. doi: 10.1016/j.jfoodeng.2005.11.024.
Belwal, T., Ezzat, S. M., Rastrelli, L., Bhatt, I. D., Daglia, M., Baldi, A., Devkota, H. P., Orhan, I. E., Patra, J. K., Das, G., Anandharamakrishnan, C., Gomez-Gomez, L., Nabavi, S. F., Nabavi, S. M., and Atanasov, A. G. A., 2018. Critical Analysis of Extraction Techniques Used for Botanicals: Trends, Priorities, Industrial Uses and Optimization Strategies. Trac- Trends Analytical Chemistry 100, 82–102. doi: 10.1016/j.trac.2017.12.018.
Benjama, O. and P. Masniyom., 2012. Biochemical composition and physicochemical properties of two red seaweeds (Gracilaria fisheri and G. tenuistipitata) from the Pattani Bay in Southern Thailand. Songklanarin Journal of Science and Technology 34(2), 223-230.
Sari et al., ALCHEMY Jurnal Penelitian Kimia, Vol. 16(1) 2020, 37-48
47
Copyright © 2020, ALCHEMY Jurnal Penelitian Kimia, ISSN 1412-4092, e ISSN 2443-4183
Camel, V., 2000. Microwave-assisted solvent extraction of environmental samples. Trac- Trends Analytical Chemistry 19, 229–248. doi: 10.1016/S0165-9936(99)00185-5.
Chakaborty, K., Joseph, D., and Praveen, N.K., 2015. Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India. Journal of Food Science Technology 54, 1924-1935. doi: 10.1007/s13197-013-1189-2
Coneac, G., GafiÅ£anu, E., HÄƒdÄƒrugÄƒ, D.I., HÄƒdÄƒrugÄƒ, PÄnzaru, I.A., Bandur, G., UrÅŸica, PÄƒunescu, V., and Gruia, A., 2008. Flavonoid contents of Propolis from the West side of Romania and correlation with the antioxidant activity. Chemical Bulletin 53(67), 1-2.
Dang, T.T., Bowyer, M.C., Van Altena, I.A., and Scarlett, C.J., 2017. Optimum conditions of microwave-assisted extraction for phenolic compounds and antioxidant capacity of the brown alga Sargassum vestitum. Separation science and Technology 53, 1711–1723. doi: 10.1080/01496395.2017.1414845.
Departemen Kesehatan R.I., 1992. Cara Pembuatan Obat Tradisional Yang Baik. Departemen Kesehatan Republik Indonesia, Jakarta.
Departemen Kesehatan Republik Indonesia, 1995. Farmakope Indonesia, Edisi IV. Departemen Kesehatan Republik Indonesia, Jakarta.
Farris, S., and Piergiovanni, L., 2009. Optimization of manufacture of almond paste cookies using response surface methodology. Journal of Food Process Engineering 32(1), 64–87. doi: 10.1111/j.1745-4530.2007.00203.x.
Haminiuk, C. W. I., Maciel, G. M., Plata-Oviedo, M. S. V., and Peralta, R. M. 2012., Phenolic compounds in fruits – An overview. International Journal of Food Science & Technology 47(10), 2023–2044. doi:10.1111/j.1365-2621.2012.03067.x.
He, Q., Du, B., and Xu, B., 2018. Extraction optimization of phenolics and antioxidants from Black Goji Berry by Accelerated Solvent Extrator using response surface methodology. Applied Sciences 8, 1905. doi: 10.3390/app8101905.
Horowitz, R.M., 1957. Flavonoids of citrus. II. Isolation of a new flavonol from lemons. Journal of The American Chemical Society 79, 6561.
Kementrian Kesehatan R.I., 2011. Suplemen II Farmakope Herbal Indonesia Edisi I. Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan, Jakarta.
Luthria, D. L., 2008. Influence of experimental conditions on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes using a pressurized liquid extractor. Food Chemistry 107(2), 745–752. doi:10.1016/j.foodchem.2007.08.074.
Maharany, F., Nurjanah., Suwandi, R., Anwar, E., and Hidayat, T., 2017. Kandungan Senyawa Bioaktif Rumput Laut Padina australis dan Eucheuma cottonii Sebagai Bahan Bahku Krim Tabir Surya. Jurnal Pengolahan Hasil Perikanan Indonesia 20(1), 10-17. doi: 10.17844/jphpi.v20i1.16553.
Pereira, L., 2018. Therapeutic and Nutritional Uses of Algae. CRC Press, Portugal.
Pew, J.C., 1948. A flavonone from Douglas-fir heartwood. Journal of The American Chemical Society 70, 3031–3034. doi: 10.1021/ja01189a059.
Putrinesia, I., Tobing, Y.P.L., Asikin, N., and Rahmalia, W., 2018. Formulasi dan uji aktivitas krim pengkelat merkuri berbahan dasar ekstrak etanol alga coklat (Sargassum sp.). Alchemy: Jurnal Penelitian Kimia 14(1), 152-163. doi:

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Detail Karya Ilmiah Dosen

Bina Lohita Sari, Triastinurmiatiningsih, Tri Saptari Haryani

Judul	:	Optimasi Metode Microwave-Assisted Extraction (MAE) untuk Menentukan Kadar Flavonoid Total Alga Coklat Padina australis
Abstrak	:	Metode Microwave-Assisted Extraction (MAE) digunakan untuk mengekstraksi senyawa flavonoid dari simplisia alga coklat Padina australis. Sampel diperoleh dari Pantai Bayah, Banten Indonesia. Kondisi optimal ekstraksi ditentukan dengan Response Surface Methodology (RSM). Desain Box-Behnken (BBD) digunakan untuk mengevaluasi pengaruh 3 faktor dengan 3 level yaitu daya microwave (300, 450, 600 watt), konsentrasi etanol (30, 50, 70%), dan waktu ekstraksi (7, 8 dan 9 menit) dengan 15 perlakuan yang berbeda. Hasil disain eksperimen dengan BBD menunjukkan kondisi ekstraksi optimum yaitu pada daya microwave 414 watt, konsentrasi etanol 50,33% dan waktu ekstraksi 7,89 menit menghasilkan kadar prediksi flavonoid sebesar nilai 0,2963%. Nilai ini mendekati nilai kadar flavonoid yang diperoleh secara eksperimental sebesar 0,2961%, pada kondisi menggunakan daya microwave sebesar 450 watt, konsentrasi etanol 50% dengan lama ekstraksi 8 menit. Berdasarkan hasil penelitian, kadar flavonoid total dapat meningkat secara signifikan dengan melakukan optimasi proses MAE menggunakan RSM. Kata kunci: Microwave-Assisted Extraction, kadar flavonoid total, alga coklat, Padina australis
Tahun	:	2020	Media Publikasi	:	Jurnal Nasional Terakreditasi A
Kategori	:	Jurnal	No/Vol/Tahun	:	16 / 12 / 2020
ISSN/ISBN	:	1412-4092/ e ISSN 2443-4183
PTN/S	:	universitas pakuan	Program Studi	:	FARMASI
Bibliography	:	Alara, O.R., Abdurahman, N.H., and Olalere, O.A., 2018. Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool. Journal of Food Measurement and Characterization 12, 1107-1122. doi: 10.1007/s11694-018-9726-3. Bas, D., and Boyaci, I.H., 2007. Modeling and optimization I: Usability of response surface methodology. Journal of Food Engineering 78(3), 836-45. doi: 10.1016/j.jfoodeng.2005.11.024. Belwal, T., Ezzat, S. M., Rastrelli, L., Bhatt, I. D., Daglia, M., Baldi, A., Devkota, H. P., Orhan, I. E., Patra, J. K., Das, G., Anandharamakrishnan, C., Gomez-Gomez, L., Nabavi, S. F., Nabavi, S. M., and Atanasov, A. G. A., 2018. Critical Analysis of Extraction Techniques Used for Botanicals: Trends, Priorities, Industrial Uses and Optimization Strategies. Trac- Trends Analytical Chemistry 100, 82–102. doi: 10.1016/j.trac.2017.12.018. Benjama, O. and P. Masniyom., 2012. Biochemical composition and physicochemical properties of two red seaweeds (Gracilaria fisheri and G. tenuistipitata) from the Pattani Bay in Southern Thailand. Songklanarin Journal of Science and Technology 34(2), 223-230. Sari et al., ALCHEMY Jurnal Penelitian Kimia, Vol. 16(1) 2020, 37-48 47 Copyright © 2020, ALCHEMY Jurnal Penelitian Kimia, ISSN 1412-4092, e ISSN 2443-4183 Camel, V., 2000. Microwave-assisted solvent extraction of environmental samples. Trac- Trends Analytical Chemistry 19, 229–248. doi: 10.1016/S0165-9936(99)00185-5. Chakaborty, K., Joseph, D., and Praveen, N.K., 2015. Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India. Journal of Food Science Technology 54, 1924-1935. doi: 10.1007/s13197-013-1189-2 Coneac, G., GafiÅ£anu, E., HÄƒdÄƒrugÄƒ, D.I., HÄƒdÄƒrugÄƒ, PÄnzaru, I.A., Bandur, G., UrÅŸica, PÄƒunescu, V., and Gruia, A., 2008. Flavonoid contents of Propolis from the West side of Romania and correlation with the antioxidant activity. Chemical Bulletin 53(67), 1-2. Dang, T.T., Bowyer, M.C., Van Altena, I.A., and Scarlett, C.J., 2017. Optimum conditions of microwave-assisted extraction for phenolic compounds and antioxidant capacity of the brown alga Sargassum vestitum. Separation science and Technology 53, 1711–1723. doi: 10.1080/01496395.2017.1414845. Departemen Kesehatan R.I., 1992. Cara Pembuatan Obat Tradisional Yang Baik. Departemen Kesehatan Republik Indonesia, Jakarta. Departemen Kesehatan Republik Indonesia, 1995. Farmakope Indonesia, Edisi IV. Departemen Kesehatan Republik Indonesia, Jakarta. Farris, S., and Piergiovanni, L., 2009. Optimization of manufacture of almond paste cookies using response surface methodology. Journal of Food Process Engineering 32(1), 64–87. doi: 10.1111/j.1745-4530.2007.00203.x. Haminiuk, C. W. I., Maciel, G. M., Plata-Oviedo, M. S. V., and Peralta, R. M. 2012., Phenolic compounds in fruits – An overview. International Journal of Food Science & Technology 47(10), 2023–2044. doi:10.1111/j.1365-2621.2012.03067.x. He, Q., Du, B., and Xu, B., 2018. Extraction optimization of phenolics and antioxidants from Black Goji Berry by Accelerated Solvent Extrator using response surface methodology. Applied Sciences 8, 1905. doi: 10.3390/app8101905. Horowitz, R.M., 1957. Flavonoids of citrus. II. Isolation of a new flavonol from lemons. Journal of The American Chemical Society 79, 6561. Kementrian Kesehatan R.I., 2011. Suplemen II Farmakope Herbal Indonesia Edisi I. Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan, Jakarta. Luthria, D. L., 2008. Influence of experimental conditions on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes using a pressurized liquid extractor. Food Chemistry 107(2), 745–752. doi:10.1016/j.foodchem.2007.08.074. Maharany, F., Nurjanah., Suwandi, R., Anwar, E., and Hidayat, T., 2017. Kandungan Senyawa Bioaktif Rumput Laut Padina australis dan Eucheuma cottonii Sebagai Bahan Bahku Krim Tabir Surya. Jurnal Pengolahan Hasil Perikanan Indonesia 20(1), 10-17. doi: 10.17844/jphpi.v20i1.16553. Pereira, L., 2018. Therapeutic and Nutritional Uses of Algae. CRC Press, Portugal. Pew, J.C., 1948. A flavonone from Douglas-fir heartwood. Journal of The American Chemical Society 70, 3031–3034. doi: 10.1021/ja01189a059. Putrinesia, I., Tobing, Y.P.L., Asikin, N., and Rahmalia, W., 2018. Formulasi dan uji aktivitas krim pengkelat merkuri berbahan dasar ekstrak etanol alga coklat (Sargassum sp.). Alchemy: Jurnal Penelitian Kimia 14(1), 152-163. doi:
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