Siti Warnasih, Uswatun Hasanah

Judul	:	PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw)
Abstrak	:	One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 °C, and kluwek extract unstable when contact with 1% H2O2 , UV light, and if kept at cold temperature (10 ËšC).
Tahun	:	2018	Media Publikasi	:	Jurnal Nasional Blm Akreditasi
Kategori	:	Jurnal	No/Vol/Tahun	:	2 / 1 / 2018
ISSN/ISBN	:	2615-3750
PTN/S	:	Universitas Pakuan	Program Studi	:	KIMIA
Bibliography	:	[1] Elbanna, K., Sarhan, O.M., Khider, M., Elmogy, M., Abulreesh, H.H., Shaaban, M.R. 2017. Microbiological, histological, and biochemical evidence for adverse effects of azo dyes on rats. Journal of Food and Drug Analysis 25 (3): 667- 680 [2] Heyne, K. 1987. Useful Plants Indonesia. Volume III. Jakarta: Sarana Wana Jaya Foundation. [3] Hilditch, T.P., William, P.N. 1964. The Chemical Constituent of Natural Fats. Chapman and Hall. London. [4] Mangunwardoyo, W., Ismaini, L., Heruwati, E.S. 2008. Analysis of fresh bioactive compound extracts of Pangung edule. Biological News 9 (3): 259-264. [5] Heruwati, E.S., Widyasari, H.E., Haluan, J. 2007. Preservation of fresh fish using picung seeds (Pangium edule Reinw). Postharvest Journal and Marine and Fisheries Biotechnology. 2 (1): 9-18 [6] Artati, E. K., Fadilah. 2007. Effect of Stirring Rotational Speed and Operating Temperature on Extraction of Tannins from Cashew with Acetone Solvents. Equilibrium 6 (1): 33-38. [7] Warnasih, S., Hasanah, U. 2018. Dyestuff Extraction from Kluwek (Pangium edule Reinw) Using Various Solvents. Ekologia (review). [8] Putra, A.A.B., Bogoriani, N.W., Diantariani, N.P., Sumadewi, N.L.U. 2014. Extraction of Natural Dyes from the Banana Plant (Musa paradiasciaca L.) by Maseration, Reflux and Sokletasi Methods. Journal of Chemistry. 8 (1): 113-119. [9] Neliyanti and Nora, I. 2014. Extraction and Stability Test of Natural Dyes from Lakum (Cayratia trifolia (L.) Domin). Journal of Chemistry FMIPA. University of Tanjungpura. 3 (2): 8693. [10] Ministry of Health of the Republic of Indonesia. 2000. General Standard Parameters of Medicinal Plant Extracts. (Issue I). Jakarta: Directorate General of Drug and Food Control. [11] Lenny, S. 2006. Flavonoida Compounds, Phenyl Propanoide and Alkaloids. MIPA Universitas Sumatera Utara. [12] Robinson, T. 1995. Organic Plant High Content. Second Edition. ITB. Bandung [13] Latifah. 2015. Group Identification of Flavanoid Compounds and Antioxidant Activity Test on Kencur Rhizome Extract (Kaempferia galanga L.) by Method of DPPH. Skripsi. Malang: State Islamic University Maulana Malik Ibrahim. [14] Harbone, J.B. 2000. Method of Phytochemical Guidance for Modern Ways to Analyze Plants. Bandung: ITB [15] Sundari, U. 2008. Comparative Test of Buah Merah Carotenoid and Tocopherol Extraction Methods. Bogor: Bogor Agricultural University. [16] Susanti, H. 2012. Determination of Total Phenolic Content of Methanol Extract Red Rose Rosella Petals (Hibicus sabdariffa Linn) With Spectrophotometric Growth Variation Place. Yogyakarta: Ahmad Dahlan University. [17] Sibuea, F.S.Y. 2015. Tannin extraction from kluwak (Pangium edule R.) using ethanol solvents and distilled water and its application as food coloring. Semarang State University. Semarang. [18] Okuda, T., Ito, H. 2011. Tannins of Constant Structure in Medicinal and Food PlantsHydrolyzable Tannins and Polyphenol Related to Tannins. Journal. ISSN 1420-3029: 2192- 2217 [19] Fajriati, I. 2001. Optimization of the Tanin Determination Method. Malang: Universitas Islam Negeri Malang. [20] Saewan, N., Jimtaison A. 2013. Photoprotection of Natural Flavanoids. Journal of Applied Pharmaceutica Science: 129-36 [21] Risnasari, I. 2002. Tanin. Medan: digital library University of North Sumatra. [22] Miksusanti, Elfita, Hotdelina, S. 2012. Antioxidant Activity and Properties of Color Stability Mixture of Ethyl Acetate Extract of Mangosteen Fruit (Garcinia mangostana L.) and Secang Wood (Caesalpinia sappan L.). Journal of Science Research. 15 (2): 60-69.
URL	:	https://journal.unpak.ac.id/index.php/jsi/article/view/1000/852