Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Oom Komala, Sri Wardatun, Lia Puspita Sari3

Judul : Effectiveness of Leaves Extract Fractions of Archidendron jiringa (Jack) I.C Nielsen Against Microbes

Abstrak :

Archidendron jiringa (Jack) I.C Nielsen) contains a lot of essential oils, saponins, alkaloids, terpenoids, steroids, tannins, glycosides, and flavonoids. Flavonoid is a secondary metabolite compound in a plant as antimicrobial. This study aims to determine the antimicrobial activity of fractions of ethanol 96% , n-hexan , ethyl acetate , and ethanol-water leaves extract. A. jiringa leaves extract were tested against Streptococus mutans, Pseudomonas aeruginosa, and Candida albicans. The research using agar disc diffusion technique for antimicrobial activity and colorimetric method to know the total of flavonoid. Chloramphenicol and ketoconazole were used as a reference standard. The result of this study shows fractions of ethanol 96% , n-hexane , ethyl acetate , and ethanol-water A. jiringa leaves extract can inhibit microbial growth of S. mutans, P. aeruginosa, and C. albicans. Total of flavonoids on the extract respectively are 1,13%, 0,494%, 2,337%, and 0,549%.   The higher percent of flavonoids would cause greater    inhibitory zone bacteria but only 0.090 for pearson correlation value. Conclusions ethyl acetate leaves extract most effective inhibit microbial growth from the other fractions.

Tahun : 2020 Media Publikasi : Jurnal Internasional

Kategori : Jurnal No/Vol/Tahun : 2 / 14 / 2019

ISSN/ISBN : 1907-9761

PTN/S : Universitas Pakuan Program Studi : BIOLOGI

Bibliography :
Anze, S.P.G., Lamsing, N., Ugwoke, C.E.C., & Ezugwu,C.O. (2017). Antimicrobial Activity of Methanol Extracts and Fractions of the Leaf and Stem Bark of Vitex Doniana Sweet (Lamiaceae). International Journal of Physical and Human Geography, 5(1),14-21.

Anonim. (2013). Farmakope Herbal Indonesia (First ed.) (pp106-107). Dep. Of Health RI, Indonesia: Jakarta.

Balouiri,M., Sadiki,M., & Ibnsouda, S.K. (2016). Methods in vitro evaluating antimicrobial activity : A review. Journal of Pharmaceutical Analysis, 6(2),71-79.

Barceloux, D.G. (2009). Djenkol Bean [Archidendron jiringa (Jack) I.C Nielsen]. Disease-a-Mouth, 55(6), 361-364.

Bakar RA, Ahmad, I., &   Sulaiman, S.F. (2012). Effect of Pithecellobium jiringa as antimicrobial agen. Bangladesh Journal of Pharmacology, 7(2), 131-134.

Bunawan, H., Dusik, L., Bunawan. S.N., & Amin, M.N. (2013). Botany, Traditional Uses, Phytochemistry and Pharmacology of Archidendron jiringa: A Review. Global J. Pharmacol, 7 (4), 474-478.

Charungchitrak, S., Petsom, A., Sangvanich,P., & Karnchanatat, A. (2011). Antifungal and antibacterial activities of lectin from the seeds of Archidendron jiringa Nielsen. Food Chemistry, 126(3),1025-1032.

Farhadi, F., Khamemeh, B., Iranshahi, M., & Milad I. (2019). Antibacterial activity of flavonoids and their structure-activity relationship : An update review. Phytotherapy Research, (33), 13-40. Doi : 10.1002/ptr.6208

Hasan, A.M. (2013). In-Vitro Antibacterial Activity Of Proanthocyanidins Against some of Pathogenic Bacterial Isolates. Al-Mustansiriyah J.Sci., 24(1),53-60

Hussin, Z.M., Osman, N.A., Harun, A., & Daud S. (2018). Phytochemical and Antimicrobial Evaluation of Pithecellobium jiringa Stem Bark Extracts. Malaysian Journal of Analytical Sciences, 22(1), 123-127. DOI: https://doi.org/10.17576/mjas-2018-2201-15

Lubis, M.Y., Marpaung, L., Siburian, R., & Nasution, M.P. (2018). Methyl Gallate from Jiringa (Archidendron jiringa) and Antioxidant Activity. Asian Journal of Pharmaceutical and Clinical Research, 11(1), 346.

Mazzola, P.G., Jozala, A.F., Novaes, L.C.L., Moriel, P. & Penna, T.C.V. (2009). Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents. BJPS, 45(2), 241-248. Retrieved from www.scielo.br/pdf/bjps/v45n2/v45n2a08.pdf

Norulaini, N.A.N., Zaidul, I.S.M., Azizi, C.Y.M., Zhari, I., Noranin, M.N., Sahena, F., & Omar, A.K.M. (2011).   Supercritical Carbon dioxide fractionation of Pithecellobium jiringa Jack seed compositions using fast gas chomatography time of flight mass spectrometry. Journal of food Process Engineering, 34(5), 1746-1758.

Onsare, J.G., & Arora, D.S. (2015). Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. J Appl Microbiol, 118(2), 313-25.

Ramli, S.(2013). Bioactivity evaluations and phytochemical characterizations of ethanolic extracts from selected mimosaceous plants endemic to Thailand. Global Journal of Pharmacology, 7 (4), 474-478.

Ventola, C.L. (2015). The Antibiotic Resistance Crisis. Part 1 : Causes and Threats. PT, 40(4), 277-283.

Xie ,Y, Yang W, Tang, Chen X & Ren I. (2015). Antibacterial activities of flavonoids: structure – activity relationship and mechanism. Current Medicinal Chemistry, 22(1), 132-149.

Yanti, Irnawati, F., Vivian, M., & Wulandari, Y.R.E. (2015). Extraction yield and antioxidant activity of biomolecule and bioactive fractions from seed and peel parts of Pithecellobium jiringa. Sch.Acad J.Biosci., 3(9), 790-795. Retrieved from

https://www.researchgate.net/publication/327136893_Extraction_yield_and_antioxidant_activity_of_biomolecule_and_bioactive_fractions_from_seed_and_peel_partsof_Pithecellobium_jiringa

Zore, G.B., Thakre, A.D., Jadhad, S., & Karuppayil, S.M. (2011).   Terpenoids inhibit Candida albicans growth by affecting membrane integrity and arrest of Cell Cycle. Phytomedicine, 18(13), 1181-90.

URL : https://ojs.jmolekul.com/ojs/index.php/jm

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Detail Karya Ilmiah Dosen

Oom Komala, Sri Wardatun, Lia Puspita Sari3

Judul	:	Effectiveness of Leaves Extract Fractions of Archidendron jiringa (Jack) I.C Nielsen Against Microbes
Abstrak	:	Archidendron jiringa (Jack) I.C Nielsen) contains a lot of essential oils, saponins, alkaloids, terpenoids, steroids, tannins, glycosides, and flavonoids. Flavonoid is a secondary metabolite compound in a plant as antimicrobial. This study aims to determine the antimicrobial activity of fractions of ethanol 96% , n-hexan , ethyl acetate , and ethanol-water leaves extract. A. jiringa leaves extract were tested against Streptococus mutans, Pseudomonas aeruginosa, and Candida albicans. The research using agar disc diffusion technique for antimicrobial activity and colorimetric method to know the total of flavonoid. Chloramphenicol and ketoconazole were used as a reference standard. The result of this study shows fractions of ethanol 96% , n-hexane , ethyl acetate , and ethanol-water A. jiringa leaves extract can inhibit microbial growth of S. mutans, P. aeruginosa, and C. albicans. Total of flavonoids on the extract respectively are 1,13%, 0,494%, 2,337%, and 0,549%. The higher percent of flavonoids would cause greater inhibitory zone bacteria but only 0.090 for pearson correlation value. Conclusions ethyl acetate leaves extract most effective inhibit microbial growth from the other fractions.
Tahun	:	2020	Media Publikasi	:	Jurnal Internasional
Kategori	:	Jurnal	No/Vol/Tahun	:	2 / 14 / 2019
ISSN/ISBN	:	1907-9761
PTN/S	:	Universitas Pakuan	Program Studi	:	BIOLOGI
Bibliography	:	Anze, S.P.G., Lamsing, N., Ugwoke, C.E.C., & Ezugwu,C.O. (2017). Antimicrobial Activity of Methanol Extracts and Fractions of the Leaf and Stem Bark of Vitex Doniana Sweet (Lamiaceae). International Journal of Physical and Human Geography, 5(1),14-21. Anonim. (2013). Farmakope Herbal Indonesia (First ed.) (pp106-107). Dep. Of Health RI, Indonesia: Jakarta. Balouiri,M., Sadiki,M., & Ibnsouda, S.K. (2016). Methods in vitro evaluating antimicrobial activity : A review. Journal of Pharmaceutical Analysis, 6(2),71-79. Barceloux, D.G. (2009). Djenkol Bean [Archidendron jiringa (Jack) I.C Nielsen]. Disease-a-Mouth, 55(6), 361-364. Bakar RA, Ahmad, I., & Sulaiman, S.F. (2012). Effect of Pithecellobium jiringa as antimicrobial agen. Bangladesh Journal of Pharmacology, 7(2), 131-134. Bunawan, H., Dusik, L., Bunawan. S.N., & Amin, M.N. (2013). Botany, Traditional Uses, Phytochemistry and Pharmacology of Archidendron jiringa: A Review. Global J. Pharmacol, 7 (4), 474-478. Charungchitrak, S., Petsom, A., Sangvanich,P., & Karnchanatat, A. (2011). Antifungal and antibacterial activities of lectin from the seeds of Archidendron jiringa Nielsen. Food Chemistry, 126(3),1025-1032. Farhadi, F., Khamemeh, B., Iranshahi, M., & Milad I. (2019). Antibacterial activity of flavonoids and their structure-activity relationship : An update review. Phytotherapy Research, (33), 13-40. Doi : 10.1002/ptr.6208 Hasan, A.M. (2013). In-Vitro Antibacterial Activity Of Proanthocyanidins Against some of Pathogenic Bacterial Isolates. Al-Mustansiriyah J.Sci., 24(1),53-60 Hussin, Z.M., Osman, N.A., Harun, A., & Daud S. (2018). Phytochemical and Antimicrobial Evaluation of Pithecellobium jiringa Stem Bark Extracts. Malaysian Journal of Analytical Sciences, 22(1), 123-127. DOI: https://doi.org/10.17576/mjas-2018-2201-15 Lubis, M.Y., Marpaung, L., Siburian, R., & Nasution, M.P. (2018). Methyl Gallate from Jiringa (Archidendron jiringa) and Antioxidant Activity. Asian Journal of Pharmaceutical and Clinical Research, 11(1), 346. Mazzola, P.G., Jozala, A.F., Novaes, L.C.L., Moriel, P. & Penna, T.C.V. (2009). Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents. BJPS, 45(2), 241-248. Retrieved from www.scielo.br/pdf/bjps/v45n2/v45n2a08.pdf Norulaini, N.A.N., Zaidul, I.S.M., Azizi, C.Y.M., Zhari, I., Noranin, M.N., Sahena, F., & Omar, A.K.M. (2011). Supercritical Carbon dioxide fractionation of Pithecellobium jiringa Jack seed compositions using fast gas chomatography time of flight mass spectrometry. Journal of food Process Engineering, 34(5), 1746-1758. Onsare, J.G., & Arora, D.S. (2015). Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. J Appl Microbiol, 118(2), 313-25. Ramli, S.(2013). Bioactivity evaluations and phytochemical characterizations of ethanolic extracts from selected mimosaceous plants endemic to Thailand. Global Journal of Pharmacology, 7 (4), 474-478. Ventola, C.L. (2015). The Antibiotic Resistance Crisis. Part 1 : Causes and Threats. PT, 40(4), 277-283. Xie ,Y, Yang W, Tang, Chen X & Ren I. (2015). Antibacterial activities of flavonoids: structure – activity relationship and mechanism. Current Medicinal Chemistry, 22(1), 132-149. Yanti, Irnawati, F., Vivian, M., & Wulandari, Y.R.E. (2015). Extraction yield and antioxidant activity of biomolecule and bioactive fractions from seed and peel parts of Pithecellobium jiringa. Sch.Acad J.Biosci., 3(9), 790-795. Retrieved from https://www.researchgate.net/publication/327136893_Extraction_yield_and_antioxidant_activity_of_biomolecule_and_bioactive_fractions_from_seed_and_peel_partsof_Pithecellobium_jiringa Zore, G.B., Thakre, A.D., Jadhad, S., & Karuppayil, S.M. (2011). Terpenoids inhibit Candida albicans growth by affecting membrane integrity and arrest of Cell Cycle. Phytomedicine, 18(13), 1181-90.
URL	:	https://ojs.jmolekul.com/ojs/index.php/jm