Judul | : | Inhibitory Activity of Alpha Glucosidase inhibitors of Bark of Ceiba pentandra Linn | |||
Abstrak | : | Bark of Kapok (Ceiba pentandra (L.)) contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. Flavonoid known to have inhibitory activity of α-glucosidase. The purpose of this study was to observe the n-hexane, ethyl acetate and water extract of bark of Ceiba pentandra (L.) to inhibitory activity of α-glucosidase. The method used for the activity measurement of the fractions was spectrophotometry UV-Vis. Water fraction analyzed by LCMS/MS to identify the chemical compounds. The pure compound suspected analyzed using molecular docking to predict the complementarity related to the model of isomaltase α-glucosidase using Autodock Vina, therefore the bond of the antidiabetic active compounds can be predicted. The results show the α-glucosidase inhibitory activity of the bark in n-hexane fraction is 4.60μg/mL, ethyl acetate fraction is 8.55μg/mL and water fraction is 5.61μg/mL. Water fraction analyzed by LCMS/MS eludated at retention time of 3.61min be fathomed as (+) catechin derivate and at retention time of 8.70min be fathomed as vavain derivate. The vavain derived compound, (+) catechin derived compound, acarbose and quercetin each shows docking score of -8.1; -8.8; -6.2 and -7.6 kkal/mol and have similarity to amino acid Glu276 bond which is a catalytic residue that plays role in hydrolysis process. The similarity of chemical bond of the active site be expected to have the same activity as α-glucosidase inhibitory agent. |
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Tahun | : | 2018 | Media Publikasi | : | Jurnal Nasional Terakreditasi A |
Kategori | : | Jurnal | No/Vol/Tahun | : | 4 / 29 / 2018 |
ISSN/ISBN | : | 2338-9427 | |||
PTN/S | : | universitas pakuan | Program Studi | : | FARMASI |
Bibliography | : | Demir, E., Serdar, G., & Sokmen, M. 2016. Comparison of some extraction methods for isolation of catechins and caffeine from Turkish Green Tea. International Journal Of Secondary Metabolite, 2(2), 16–25. |
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URL | : | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/issue/view/139 |