Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

Vicri Syihabudin, Bina Lohita Sari, Novi Fajar Utami, Niki Aulia Apriliani

Judul : Inhibitory Activity of Alpha Glucosidase inhibitors of Bark of Ceiba pentandra Linn
Abstrak :

Bark of Kapok (Ceiba pentandra (L.)) contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. Flavonoid known to have inhibitory activity of α-glucosidase. The purpose of this study was to observe the n-hexane, ethyl acetate and water extract of bark of Ceiba pentandra (L.) to inhibitory activity of α-glucosidase. The method used for the activity measurement of the fractions was spectrophotometry UV-Vis. Water fraction analyzed by LCMS/MS to identify the chemical compounds. The pure compound suspected analyzed using molecular docking to predict the complementarity related to the model of isomaltase α-glucosidase using Autodock Vina, therefore the bond of the antidiabetic active compounds can be predicted. The results show the α-glucosidase inhibitory activity of the bark in n-hexane fraction is 4.60μg/mL, ethyl acetate fraction is 8.55μg/mL and water fraction is 5.61μg/mL. Water fraction analyzed by LCMS/MS eludated at retention time of 3.61min be fathomed as (+) catechin derivate and at retention time of 8.70min be fathomed as vavain derivate. The vavain derived compound, (+) catechin derived compound, acarbose and quercetin each shows docking score of -8.1; -8.8; -6.2 and -7.6 kkal/mol and have similarity to amino acid Glu276 bond which is a catalytic residue that plays role in hydrolysis process. The similarity of chemical bond of the active site be expected to have the same activity as α-glucosidase inhibitory agent.
Key words: α-glucosidase; AutoDock Vina; LCMS/MS; vavain derivative

Tahun : 2018 Media Publikasi : Jurnal Nasional Terakreditasi A
Kategori : Jurnal No/Vol/Tahun : 4 / 29 / 2018
ISSN/ISBN : 2338-9427
PTN/S : universitas pakuan Program Studi : FARMASI
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