Repository Universitas Pakuan

Detail Karya Ilmiah Dosen

**Anna Muthia Solihaa), Leny Heliawatia*), Siti Warnasih A)**

Judul : IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS FROM ENDOPHYTIC BACTERIAL EXTRACT OF GREEN GRASS CINCAU PLANT (PREMNA OBLONGIFOLIA MERR)

Abstrak :
Green grass jelly (Premna oblongifolia Merr) is a plant that belongs to the Verbenaceae family with the Premna genus. The genus Premna
has about 200 species in the Verbenaceae family which are spread in tropical to subtropical areas such as Asia, Africa, and Australia. This
plant has potential as antibacterial, antipyretic, anti-inflammatory and antioxidant. This research was conducted to make an extract of
endophytic bacteria obtained from green grass jelly plants by maceration method. The extract obtained was tested for its antibacterial activity
by disc diffusion method. Extracts with the highest antibacterial activity were identified by FTIR and GCMS. The results showed that the
methanol extract of endophytic bacteria of green grass jelly plants had broad spectrum antibacterial activity because it was able to inhibit the
growth of Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Pseudomonas aeruginosa). The results of FTIR and GCMS
showed that the active compounds contained in the endophytic bacteria of green grass jelly plants were thought to be Nerolidol compounds
or 3,7,11-trimethyl-1,6,10-dokatrien-3-ol which are secondary metabolites of the sesquiterpene alcohol group and play a role in as an
antibacterial.

Tahun : 2021 Media Publikasi : Jurnal Nasional Blm Akreditasi

Kategori : Jurnal No/Vol/Tahun : 2 / 1 / 2021

ISSN/ISBN : p-ISSN: 2776-1711

PTN/S : universitas pakuan Program Studi : PENDIDIKAN ILMU PENGETAHUAN ALAM

Bibliography :
[1] K. Rekha, Pandey Richa, K. Suresh Babu and J.
Madhusudana Rao. 2015. A Phytochemistry of the
Genus Premna: A Review. Division of Natural
Product Chemistry. India: Indian Institute of
Chemical Technology.
[2] Hutapea JR, and Djumidi. 2001. Inventory of
Indonesian Medicinal Plants. Center for Health
Research and Development, Ministry of Health,
Republic of Indonesia, Jakarta.
[3] Brader G, Compant S, Mitter B, Trognitz F,
Sessitsch A. 2014. Metabolic Potential of
Endophytic Bacteria. International Journal of
Current Opinion in Biotechnology. 27:30-37
[4] P. Citroreksoko, A. Taufik, A. Murharini, S.
Purawisastra, and Y. Suchyadi, Kimia Terapan, 1st
ed. Jakarta: Universitas Terbuka, 2012.
[5] Stone JK, Bacon CW, White JF Jr., 2000, An
overview of endophtic microbes: endophytism.
Microbial endophytes. New York: Marcel Dekker,
Inc.3- 29

URL : https://drive.google.com/file/d/1dAWN2HakFDArqFjYqi1J4ot6XobqrnwZ/view

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Detail Karya Ilmiah Dosen

**Anna Muthia Solihaa), Leny Heliawatia*), Siti Warnasih A)**

Judul	:	IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS FROM ENDOPHYTIC BACTERIAL EXTRACT OF GREEN GRASS CINCAU PLANT (PREMNA OBLONGIFOLIA MERR)
Abstrak	:	Green grass jelly (Premna oblongifolia Merr) is a plant that belongs to the Verbenaceae family with the Premna genus. The genus Premna has about 200 species in the Verbenaceae family which are spread in tropical to subtropical areas such as Asia, Africa, and Australia. This plant has potential as antibacterial, antipyretic, anti-inflammatory and antioxidant. This research was conducted to make an extract of endophytic bacteria obtained from green grass jelly plants by maceration method. The extract obtained was tested for its antibacterial activity by disc diffusion method. Extracts with the highest antibacterial activity were identified by FTIR and GCMS. The results showed that the methanol extract of endophytic bacteria of green grass jelly plants had broad spectrum antibacterial activity because it was able to inhibit the growth of Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Pseudomonas aeruginosa). The results of FTIR and GCMS showed that the active compounds contained in the endophytic bacteria of green grass jelly plants were thought to be Nerolidol compounds or 3,7,11-trimethyl-1,6,10-dokatrien-3-ol which are secondary metabolites of the sesquiterpene alcohol group and play a role in as an antibacterial.
Tahun	:	2021	Media Publikasi	:	Jurnal Nasional Blm Akreditasi
Kategori	:	Jurnal	No/Vol/Tahun	:	2 / 1 / 2021
ISSN/ISBN	:	p-ISSN: 2776-1711
PTN/S	:	universitas pakuan	Program Studi	:	PENDIDIKAN ILMU PENGETAHUAN ALAM
Bibliography	:	[1] K. Rekha, Pandey Richa, K. Suresh Babu and J. Madhusudana Rao. 2015. A Phytochemistry of the Genus Premna: A Review. Division of Natural Product Chemistry. India: Indian Institute of Chemical Technology. [2] Hutapea JR, and Djumidi. 2001. Inventory of Indonesian Medicinal Plants. Center for Health Research and Development, Ministry of Health, Republic of Indonesia, Jakarta. [3] Brader G, Compant S, Mitter B, Trognitz F, Sessitsch A. 2014. Metabolic Potential of Endophytic Bacteria. International Journal of Current Opinion in Biotechnology. 27:30-37 [4] P. Citroreksoko, A. Taufik, A. Murharini, S. Purawisastra, and Y. Suchyadi, Kimia Terapan, 1st ed. Jakarta: Universitas Terbuka, 2012. [5] Stone JK, Bacon CW, White JF Jr., 2000, An overview of endophtic microbes: endophytism. Microbial endophytes. New York: Marcel Dekker, Inc.3- 29
URL	:	https://drive.google.com/file/d/1dAWN2HakFDArqFjYqi1J4ot6XobqrnwZ/view