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DINA DYAH SAPUTRI

Judul : In Vitro Assay to Investigate Ethanol Extract of Ipomoea batatas Leaves as Potential Biofungicide for Controlling Fusarium

Abstrak :
Abstract. Fusarium sp. has been known as a pathogenic agent causing fruit rots in chili. It is expected to significantly decrease annual chili production rates. One plant potentially utilized for controlling Fusarium is purple sweet potato (Ipomoea batatas L.). Purple sweet potato contain active compounds in the form of flavonoids, which has been previously revealed to perform antifungal activity. This study aimed to determine antifungal activity of purple sweet potato (Ipomoea batatas L.) leaves as a bio fungicide for inhibiting the growth of Fusarium sp.-caused chili fruit rots. This work was conducted by testing the ethanol-extracted purple sweet potato leaves extracts against Fusarium sp. with in vitro assays. It was conducted by applying disc-diffusion method to observe the antifungal activity against Fusarium sp. This study showed inhibitory ability of the leaves extract against the growth of Fusarium sp. to range between 46.9% - 89.2%. Besides, in vitro treatment of the leaves extract at 40% concentration was discovered to significantly inhibit the incubation time of Fusarium sp. fruit rot compared to other treatment groups. These findings suggested the leaves extract at 40% concentration to be a potential bio fungicide for controlling Fusarium fruit rots on chili.

Keywords: bio fungicide, chili, Fusarium, Ipomoea batatas

Tahun : 2019 Media Publikasi : Prosiding

Kategori : Prosiding No/Vol/Tahun : 1 / 468 / 2020

ISSN/ISBN : 10.1088/1755-1315/468/1/

PTN/S : UNIVERSITAS PAKUAN Program Studi : PENDIDIKAN BIOLOGI

Bibliography :
[1]     Utami A W A 2018 Isolation and Identification of Fungi Causing Withered Disease in Cayenne Pepper (Capsicum frutescens) Plants in Bogor Journal of Pedagogia. 10 (1): 34-43

[2]     Pandey K K and Gupta R C 2013 Virulence Analysis of Fusarium oxysporum f.sp. lycopersici Causing Tomato Wilt in India J. Mycol. Plant. Pathol. 43: 409-413.

[3]     Tando E 2018 Potential of Secondary Metabolite Compounds in Soursop (Annona murricata) and Srikaya (Annona squamosa) as Vegetable Pesticides for Control of Pests and Disease in Plants Biotropika Journal. 6 (1): 21-27

[4]     Dalimunthe C I and Rachmawan A 2017 Prospects of Utilization of Plant Secondary Metabolites as Vegetable Pesticides for Control of Pathogens in Rubber Plants. Warta Perkaretan. 36 (1): 15-28

[5]     Ginting E, Utomo J S, Yulifianti R and Jusuf M 2011. Potential of Purple Sweet Potato as Functional Food Food Crop Science and Technology. 6(1): 116-138.

[6]     Saputri D D and Kurniasih S 2018 Phytochemical Screening and Analysis of Gass Cromatography – Mass Spectrometer (GC-MS) Ethanol Extract of Purple Sweet Potato (Ipomoea batatas L.) Leaves. Research Report. University of Pakuan, Bogor.

[7]     Saputri D D 2018 Antifungal Activity of Purple Sweet Potato (Ipomoea batatas L.) Leaf Extract Against Fusarium oxysporum Pedagogia Journal. 10 (1): 84-91.

[8]     Azwanida N N 2015 A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation Med. Aromat Plants. 4 (3) : 1-6 ISSN: 2167-0412 doi:10.4172/2167-0412.1000196.

[9]     Hidayati A S and Harjono 2017 Antibacterial Activity Test of Babadotan (Ageratum conyzoides L.) Leaf Extract Cream in Ethanol Solvent. MIPA Journal. 4 (1).

[10]   Harjosuwono B A, Arnata I W and Puspawati G A K D. 2011. Design of Theory Experiment, SPSS and Excel Aplications. Malang: Lintas Kata Publishing.

[11]   Berlian Z, Aimi F and Lestari W 2016 Antifungal Activity Ocimum americanum L. Leaf Extract Against Fusarium oxysporum Schleect Biota Jurnal. 2 (1).

[12] Setyaningsih I, Desniar and Purnamasari E 2012 Antimicrobes of Chaetoceros gracilis which are Cultivated with Different Light Akuatika Journal. 3 (2): 183.

[13]   Alfiah R R, Khotima S and Turnip M 2015 Activities of Methanol Extract of Sambung Rambat (Micrania micrantha Kunth) on Candida albicans Fungus Growth Protobiont Journ. 4 (1): 53.

[14]   Dani J and Rosmaiti 2015 The Effect of Concentration and Duration of Soaking Colchicine on Watermelon (Citrullus lannatus) Seeds on Plants Diversity Agrosamudra Journal. 2 (2 :10-18.

[15]   Riansyah Y, Mulqie L and Choesrina L 2015 Antiinflamation Activity Examination of Sweet Potato Leaf Ethanol Extract on Wistar Rat Male Research Report. University of Islam, Bandung.

[16]   Soekarno, Bonny P W, Surono and Marhaenis E 2012 Potential of Kangkung Extract as Biofungicide to Control Fusarium Fruit Rot Disease in Tomato Fitopatologi Journal. 8 (5): 121-127.

[17]   Muthukumaran P, Padmapriya P, Salomi S, Umamaheswari R, Kalaiarasan P and Malarvizhi C 2011 In Vitro Antimicrobial Activity of Leaf Powder Asian J Pharm Res. 1 (4): 108-110.

URL : https://iopscience.iop.org/issue/1755-1315/468/1

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Detail Karya Ilmiah Dosen

DINA DYAH SAPUTRI

Judul	:	In Vitro Assay to Investigate Ethanol Extract of Ipomoea batatas Leaves as Potential Biofungicide for Controlling Fusarium
Abstrak	:	Abstract. Fusarium sp. has been known as a pathogenic agent causing fruit rots in chili. It is expected to significantly decrease annual chili production rates. One plant potentially utilized for controlling Fusarium is purple sweet potato (Ipomoea batatas L.). Purple sweet potato contain active compounds in the form of flavonoids, which has been previously revealed to perform antifungal activity. This study aimed to determine antifungal activity of purple sweet potato (Ipomoea batatas L.) leaves as a bio fungicide for inhibiting the growth of Fusarium sp.-caused chili fruit rots. This work was conducted by testing the ethanol-extracted purple sweet potato leaves extracts against Fusarium sp. with in vitro assays. It was conducted by applying disc-diffusion method to observe the antifungal activity against Fusarium sp. This study showed inhibitory ability of the leaves extract against the growth of Fusarium sp. to range between 46.9% - 89.2%. Besides, in vitro treatment of the leaves extract at 40% concentration was discovered to significantly inhibit the incubation time of Fusarium sp. fruit rot compared to other treatment groups. These findings suggested the leaves extract at 40% concentration to be a potential bio fungicide for controlling Fusarium fruit rots on chili. Keywords: bio fungicide, chili, Fusarium, Ipomoea batatas
Tahun	:	2019	Media Publikasi	:	Prosiding
Kategori	:	Prosiding	No/Vol/Tahun	:	1 / 468 / 2020
ISSN/ISBN	:	10.1088/1755-1315/468/1/
PTN/S	:	UNIVERSITAS PAKUAN	Program Studi	:	PENDIDIKAN BIOLOGI
Bibliography	:	[1] Utami A W A 2018 Isolation and Identification of Fungi Causing Withered Disease in Cayenne Pepper (Capsicum frutescens) Plants in Bogor Journal of Pedagogia. 10 (1): 34-43 [2] Pandey K K and Gupta R C 2013 Virulence Analysis of Fusarium oxysporum f.sp. lycopersici Causing Tomato Wilt in India J. Mycol. Plant. Pathol. 43: 409-413. [3] Tando E 2018 Potential of Secondary Metabolite Compounds in Soursop (Annona murricata) and Srikaya (Annona squamosa) as Vegetable Pesticides for Control of Pests and Disease in Plants Biotropika Journal. 6 (1): 21-27 [4] Dalimunthe C I and Rachmawan A 2017 Prospects of Utilization of Plant Secondary Metabolites as Vegetable Pesticides for Control of Pathogens in Rubber Plants. Warta Perkaretan. 36 (1): 15-28 [5] Ginting E, Utomo J S, Yulifianti R and Jusuf M 2011. Potential of Purple Sweet Potato as Functional Food Food Crop Science and Technology. 6(1): 116-138. [6] Saputri D D and Kurniasih S 2018 Phytochemical Screening and Analysis of Gass Cromatography – Mass Spectrometer (GC-MS) Ethanol Extract of Purple Sweet Potato (Ipomoea batatas L.) Leaves. Research Report. University of Pakuan, Bogor. [7] Saputri D D 2018 Antifungal Activity of Purple Sweet Potato (Ipomoea batatas L.) Leaf Extract Against Fusarium oxysporum Pedagogia Journal. 10 (1): 84-91. [8] Azwanida N N 2015 A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation Med. Aromat Plants. 4 (3) : 1-6 ISSN: 2167-0412 doi:10.4172/2167-0412.1000196. [9] Hidayati A S and Harjono 2017 Antibacterial Activity Test of Babadotan (Ageratum conyzoides L.) Leaf Extract Cream in Ethanol Solvent. MIPA Journal. 4 (1). [10] Harjosuwono B A, Arnata I W and Puspawati G A K D. 2011. Design of Theory Experiment, SPSS and Excel Aplications. Malang: Lintas Kata Publishing. [11] Berlian Z, Aimi F and Lestari W 2016 Antifungal Activity Ocimum americanum L. Leaf Extract Against Fusarium oxysporum Schleect Biota Jurnal. 2 (1). [12] Setyaningsih I, Desniar and Purnamasari E 2012 Antimicrobes of Chaetoceros gracilis which are Cultivated with Different Light Akuatika Journal. 3 (2): 183. [13] Alfiah R R, Khotima S and Turnip M 2015 Activities of Methanol Extract of Sambung Rambat (Micrania micrantha Kunth) on Candida albicans Fungus Growth Protobiont Journ. 4 (1): 53. [14] Dani J and Rosmaiti 2015 The Effect of Concentration and Duration of Soaking Colchicine on Watermelon (Citrullus lannatus) Seeds on Plants Diversity Agrosamudra Journal. 2 (2 :10-18. [15] Riansyah Y, Mulqie L and Choesrina L 2015 Antiinflamation Activity Examination of Sweet Potato Leaf Ethanol Extract on Wistar Rat Male Research Report. University of Islam, Bandung. [16] Soekarno, Bonny P W, Surono and Marhaenis E 2012 Potential of Kangkung Extract as Biofungicide to Control Fusarium Fruit Rot Disease in Tomato Fitopatologi Journal. 8 (5): 121-127. [17] Muthukumaran P, Padmapriya P, Salomi S, Umamaheswari R, Kalaiarasan P and Malarvizhi C 2011 In Vitro Antimicrobial Activity of Leaf Powder Asian J Pharm Res. 1 (4): 108-110.
URL	:	https://iopscience.iop.org/issue/1755-1315/468/1