A I Putri, Prastyono, L Haryjanto, T Herawan, A Fauzi, E Izudin, A Setiawan, Suprihati, R Hartono, D Priatna

Judul	:	Effect of methylisothiazolinone biocide in tissue culture sterilization of Casuarina equisetifolia
Abstrak	:	In this study, modification of tissue culture sterilization techniques using methylisothiazolinone bocide (MB) in liquid and solid medium was used to evaluate the acquisition of axenic Casuarina equisetifolia culture in vitro with natureexplant sources. One experimental unit had 12treatment combinations with 20replications of explants in each treatment. Acquisition of the best axenic culture of C. equisetifolia after 30 days of incubation was in a sterilization combination for 3 days shaking out in liquid WPM medium with 8 mg/l MBand in solid WPM medium with 6 mg/l MB.C. equisetifoliashowsa good growth response in WPM medium without the addition of exogenous growth hormone. The first indication of axillary shoot explants were elongation response within 15 days of culture initiation. Shoot tips changed in color from dark green to a more obvious transparent green and the internode sections became swollen and barrel-shaped. Single shoot formation from axillary buds at basal nodes and sprouted lateral buds were visible within 30 days. The 90%of shoot formation in the axenic culture of Casuarina equisetifolia werefrom lateral buds.
Tahun	:	2020	Media Publikasi	:	Prosiding
Kategori	:	Prosiding	No/Vol/Tahun	:	012007 / 522 / 2020
ISSN/ISBN	:	17551315
PTN/S	:	Universitas Pakuan	Program Studi	:	PENDIDIKAN KEPENDUDUKAN DAN LINGKUNGAN HIDUP
Bibliography	:	Mousavi SMM, Hosseini SZ, Resalati H, Mahdavi S and Garmaroody ER 2013 Papermaking Potential of Rapeseed Straw, A New Agricultural-Based Fiber Source Journal of Cleaner Production 52 420-424 CrossrefGoogle Scholar [2]Lal PS, Sharma A and Bist V 2013 Pine Needle - An Evaluation of Pulp and Paper Making Potential Journal of Forest Products & Industries 2 42-47 Google Scholar [3]Samariha A, Kiaei M, Talaeipour M and Nemati M 2011 Anatomical Structural Differences Between Branch and Trunk In Ailanthus Altissima Wood Indian Journal of Science and Technology 4 1676-1678 CrossrefGoogle Scholar [4]Turnbull JW 1990 Taxonomy and genetic variation in casuarinas ed M.H. J. L. El-Lakany, J.W. Turnbull and Brewbaker (Cairo: Desert Development Center, American University in Cairo) Advances In Casuarina Research and Utilization 1-11 ref.35 Google Scholar [5]Geary TF 1984 Tropical Tree Seed Manual. ed JA Vozzo (U.S Department of Agriculture, Forest Service, University of Virginia) Casuarina equisetifolia L, Part II-Species Discription. 378-381 Google Scholar [6]Kannan CS, Warrier EV and Singh BG 2015 Screening of Clones of Casuarina Equisetifolia for Pulping Traits Using Wood Fibre Characteristics International Journal of Current Research and Review (JCRR) 7 64-71 Google Scholar [7]Shukry N, EI-Kalyoubi SF and Hassan ME 1999 Pulping of Casuarina glauca with ASAM - An Environmental Friendly Process Journal of Scientific and Industrial Research 58 799-806 Google Scholar [8]Webster SA, Mitchell SA and Achmad MH 2003 A Novel Surface Sterilization Method for Reducing Fungal and Bacterial Contamination of Field Grown Medicinal Explants Intended For In Vitro Culture (UWI, Mona, Kingston: Biotechnology Centre) Google Scholar [9]Odutayo OI, Annusa NA, Okutade OO and Ogunsanwo YR 2007 Determination of the Sources of Microbial Contaminants of Cultured Plant Tissues Plant Pathology Journal 6 77-81 CrossrefGoogle Scholar [10]Qin F, Shinozaki K and Yamaguchi-Shinozaki K 2011 Achievements and Challenges in Understanding Plant Abiotic Stress Responses and Tolerance Plant & Cell Physiology 9 1569-1582 CrossrefGoogle Scholar [11]Felek W, Mekibib F and Admassu B 2015 Optimization of Explants Surface Sterilization Condition for Field Grown Peach (Prunus persica L. Batsch. Cv. Garnem) Intended for In Vitro Culture Academic Journal. 14 657-660 Google Scholar [12]George MW and Tripepi RR 2001 Plant Preservative Mixture™ Can Affect Shoot Regeneration from Leaf Explants of Chrysanthemum, European Birch, and Rhododendron HortiScience 36 768-769 CrossrefGoogle Scholar [13]Daud NH, Jayaraman S and Mohamed R 2012 An Improved Surface Sterilization Technique for Introducing Leaf, Nodal and Seed Explants of Aquilaria malaccensis from Field Sources Into Tissue Culture Asia-Pacific Journal of Molecular Biology and Biotechnology 20 55-58 Google Scholar [14]Silveiraa SS, Cordeiro-Silvab R, Degenhardt-Goldbachc J and Quoirinb M 2015 Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments Brazilian Journal Biology Google Scholar [15]McCown BH and Lloyd G 1980 Commercially-Feasible Micropropagation of Mountain Laurel, Kalmia latifolia, by use of shoot-tip culture Combined Proceedings, International Plant Propagators' Society 0 421-427 Google Scholar [16]George EF and Debergh PC 2008 Plant Propagation by Tissue Culture Volume 1. The Bacground 3 ed EF George and MA Hall (Netherlands: Springer) Micropropagation: Uses and Methods 2-28 Google Scholar [17]Shen X, Castle WS and Gmitter FG 2010 In Vitro Shoot Proliferation and Root Induction of Shoot Tip Explants from Mature Male Plants of Casuarina cunninghamiana Miq. Horticultura Science: A Publication of the American Society for Horticultural Science 45 797-800 Google Scholar [18]Wegayehu F, Mekbib F and Admassu B 2015 Optimization of Explants Surface Sterilization Condition for Field Grown Peach (Prunus persica L. Batsch. Cv. Garnem) Intended for In Vitro Culture African Journal of Biotechnology 14 657-660 CrossrefGoogle Scholar [19]Ervin GN and Wetzel RG 2002 Effects of Sodium Hypochlorite Sterilization and Dry Cold Storage on Germination of Juncus effusus L. Wetlands 22 191-195 CrossrefGoogle Scholar [20]Pahnwar F. 2005 Acclimatization and Establishment of Micropropagation Plant Google Scholar [21]Seth R, Kendurkar S. and Nadgauda R. 2007 In vitro Clonal Propagation of Casuarina equisetifolia Forst. from Mature Tree-Derived Explants Current Science 92 287-290 Google Scholar [22]Ali A, Ahmad T, Abbasi NA and Hafiz IA 2009 Effect of Different Concentrations of Auxins on In Vitro Rooting of Olive Cultivar Moraiolo Journal Botany 41 1223-1231 Google Scholar
URL	:	https://iopscience.iop.org/article/10.1088/1755-1315/522/1/012008